The effect of a lipopolysaccharide extracted from Escherichia coli on some innate immunity indices of albino rats

Abstract

Escherichia coli is wide world distribution enteric bacteria that cause different diseases. Many pathogenic and toxic effects may relate to it is lipopolysaccharide (LPS) which is part of bacterial outer membrane bearing endotoxic activity released after cell lysis. The LPS have bi-action either as potent activators of the immune system or injured tissue producing inflammation, dissemination intravascular coagulation, hypotension which finally end by endotoxic shock followed by death, the LPS also can induce the stimulation of phagocytic cells and increase its ability to kill any invader through its direct effect on activation of the respiratory burst. This manuscript aimed to study the modulatory effect of extracted E. coli LPS on some innate criteria of albino rats for that, either extracted E. coli LPS (ELPS) or standard E.coli O55:B5 LPS (SLPS) was injected intraperitoneally or intravenously by two different doses. Rats show transient leukopenia, lymphocytopenia, granulocytopenia, and midocytopenia (monocytes, eosinophils, and basophils) followed by leukocytosis, lymphocytosis, granulocytosis, and midocytosis at 24 hours, while different responses noted in WBCs according to different routes, doses, or types of LPS used. The existing study concluded that extracted E. coli LPS from (local strain) was able to elevation the phagocytic and respiratory burst of blood leukocytes with different effects of low and high dose, route of injection on total and differential white blood cells. The extracted E. coli LPS (local strain) can be used to produce immunomodulation in rats.