The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Blanquer, Andreu; Musı́lková, Jana; Filová, Elena; Táborská, Johanka; Brynda, Eduard; Riedel, Tomáš; Klápšťová, Andrea; Jenčová, Věra; Müllerová, Jana; Košťáková, Eva Kuželová; Procházková, R; Bačáková, Lucie

doi:10.3390/nano11020457

Cited by 9 publications

(26 citation statements)

References 53 publications

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“… 14 , 15 The fibrin coating was also shown to serve as an excellent substrate for cell growth on PLA nanofibers 16 and on PLCL/PCL nanofibrous membranes. 17 We previously reported that NF with fibrin containing 20% of hPL enhanced proliferation and differentiation of human keratinocytes. 17 However, the rather low activity of incorporated growth factors from the hPL reduced the overall effect.…”

Section: Introductionmentioning

confidence: 93%

PLCL/PCL Dressings with Platelet Lysate and Growth Factors Embedded in Fibrin for Chronic Wound Regeneration

Táborská¹,

Blanquer²,

Brynda³

et al. 2023

IJN

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Introduction The formation of diabetic ulcers (DU) is a common complication for diabetic patients resulting in serious chronic wounds. There is therefore, an urgent need for complex treatment of this problem. This study examines a bioactive wound dressing of a biodegradable electrospun nanofibrous blend of poly(L-lactide-co-ε-caprolactone) and poly(ε-caprolactone) (PLCL/PCL) covered by a thin fibrin layer for sustained delivery of bioactive molecules. Methods Electrospun PLCL/PCL nanofibers were coated with fibrin-based coating prepared by a controlled technique and enriched with human platelet lysate (hPL), fibroblast growth factor 2 (FGF), and vascular endothelial growth factor (VEGF). The coating was characterized by scanning electron microscopy and fluorescent microscopy. Protein content and its release rate and the effect on human saphenous vein endothelial cells (HSVEC) were evaluated. Results The highest protein amount is achieved by the coating of PLCL/PCL with a fibrin mesh containing 20% v/v hPL (NF20). The fibrin coating serves as an excellent scaffold to accumulate bioactive molecules from hPL such as PDGF-BB, fibronectin (Fn), and α-2 antiplasmin. The NF20 coating shows both fast and a sustained release of the attached bioactive molecules (Fn, VEGF, FGF). The dressing significantly increases the viability of human saphenous vein endothelial cells (HSVECs) cultivated on a collagen-based wound model. The exogenous addition of FGF and VEGF during the coating procedure further increases the HSVECs viability. In addition, the presence of α-2 antiplasmin significantly stabilizes the fibrin mesh and prevents its cleavage by plasmin. Discussion The NF20 coating supplemented with FGF and VEGF provides a promising wound dressing for the complex treatment of DU. The incorporation of various bioactive molecules from hPL and growth factors has great potential to support the healing processes by providing appropriate stimuli in the chronic wound.

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Section: Introductionmentioning

confidence: 93%

PLCL/PCL Dressings with Platelet Lysate and Growth Factors Embedded in Fibrin for Chronic Wound Regeneration

Táborská¹,

Blanquer²,

Brynda³

et al. 2023

IJN

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“…Nanofibers based on PBSu [ 15 ], PLCL/PCL [ 163 ], PLA/Hypericum perforatum oil (HPO) [ 21 ], PLA/GO/Q [ 162 ], PLA/silver (I)-diclofenac complex with (2-methylimidazole) [ 168 ], and PBAT/PCL [ 22 ] are few examples of polyester-based materials successfully obtained via the electrospinning method.…”

Section: Wound Dressingsmentioning

confidence: 99%

“…The most common solvent mixtures used are chloroform:methanol, chloroform:ethanol, and dichloromethane (DCM):methanol at 90:10, 80:20, and 70:30 solvent ratios, respectively, for electrospinning of PBSu/food-grade agents [ 15 ]; DCM/N, N-dimethylformamide (DMF) at a ratio of 1:9 to obtain PLA/graphene oxide (GO)/quercetin (Q) electrospun scaffolds [ 162 ]; and DCM/DMF (7:3, v / v ) and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP)/ethanol (7:3, v / v ) for preparing encapsulated antimicrobial agents in PLA/PEO/PVP matrices [ 16 ]. The addition of acetic acid to the mixture of chloroform:ethanol to dissolve the PLCL/PCL copolymer prevented the formation of beads, and led to fibers with diameters in the range of 200 nm to 2.8 µm [ 163 ]. There were also reported nanofibers with diameters of 1.68 ± 0.58 µm and 1.51 ± 0.64 µm in the case of electrospun PLA/HPO [ 21 ], 1.107–1.243 µm in the case of PLA/GO/Q [ 162 ], and 1.9 ± 0.5 μm in the case of PHB/PCL modified with hybrid melanin–TiO 2 nanostructures [ 18 ], which are considered as adequate for cell adhesion and attachment.…”

Section: Wound Dressingsmentioning

confidence: 99%

“…For example, low- and high-molecular-weight PLCL masses were investigated as blend adhesives in healing, and were comparable with a polyurethane bandage [ 171 ]. Nanofibrous membranes based on PLCL/PCL and platelet lysate (PL) containing a mixture of various growth factors are a new therapeutic approach for wound healing to stimulate skin regeneration and the proliferation and differentiation of keratinocytes [ 163 ]. It was found that the composition with a 50:50 mass ratio of components showed a high adhesion strength, and was proposed for replacement of conventional bandage adhesives.…”

Section: Wound Dressingsmentioning

confidence: 99%

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Special Features of Polyester-Based Materials for Medical Applications

Darie-Niță¹,

Râpă

Frąckowiak

2022

Polymers

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This article presents current possibilities of using polyester-based materials in hard and soft tissue engineering, wound dressings, surgical implants, vascular reconstructive surgery, ophthalmology, and other medical applications. The review summarizes the recent literature on the key features of processing methods and potential suitable combinations of polyester-based materials with improved physicochemical and biological properties that meet the specific requirements for selected medical fields. The polyester materials used in multiresistant infection prevention, including during the COVID-19 pandemic, as well as aspects covering environmental concerns, current risks and limitations, and potential future directions are also addressed. Depending on the different features of polyester types, as well as their specific medical applications, it can be generally estimated that 25–50% polyesters are used in the medical field, while an increase of at least 20% has been achieved since the COVID-19 pandemic started. The remaining percentage is provided by other types of natural or synthetic polymers; i.e., 25% polyolefins in personal protection equipment (PPE).

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“…A platelet concentrate was prepared via the centrifuge separation of platelets from human buffy coats obtained from 4 patients. The experimental procedure was previously described by Blanquer et al [7]. Briefly, the buffy coats from the 4 patients were mixed and centrifuged to separate the platelet concentrate from the red blood cells and leukocytes.…”

Section: Platelet Lysate Preparationmentioning

confidence: 99%

The co-culture of keratinocytes and fibroblasts on a MultI-layered polyester nanofibrous membrane enriched with platelet lysate

Blanquer

Filová

JENČOVÁ

et al. 2021

NANOCON 2021 Conference Proeedings

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The prevalence of chronic wounds is increasing due to the population ageing and specific illnesses like diabetes mellitus and vascular diseases. Nanofibrous membranes fabricated using synthetic polymers are promising materials to enhance skin wound healing. PCL and PVA membranes are being studied to be used as scaffolds for skin tissue engineering and hydrogels for controlled drug delivery, respectively. The present study considers the development of a multi-layered membrane made of PCL and PVA loaded with platelet lysate (PL). PCL nanofibers allowed cell adhesion and growth, whereas PVA acted as a hydrogel that releases the bioactive compounds of platelet lysate. The cytocompatibility of the membranes containing PL and without it was demonstrated on two cell types involved in wound healing, i.e. keratinocytes and fibroblasts. Both cell types were able to adhere and proliferate on the membranes. In addition, the membrane containing PL enhanced the proliferation of fibroblasts. A co-culture study was also performed by seeding each cell type on one side of the membrane. The cells were co-cultured for 7 days and the results showed that PL increased the proliferation of cells achieving a monolayer of keratinocytes or fibroblasts on each side of the membrane. Thus, the beneficial effect of PCL-PVA+PL membranes on monocultures and co-cultures of skin cells was demonstrated, and these membranes can be considered potential scaffolds for treatment of chronic wounds.

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The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Cited by 9 publications

References 53 publications

PLCL/PCL Dressings with Platelet Lysate and Growth Factors Embedded in Fibrin for Chronic Wound Regeneration

PLCL/PCL Dressings with Platelet Lysate and Growth Factors Embedded in Fibrin for Chronic Wound Regeneration

Special Features of Polyester-Based Materials for Medical Applications

The co-culture of keratinocytes and fibroblasts on a MultI-layered polyester nanofibrous membrane enriched with platelet lysate

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