The effect of a porcine reproductive and respiratory syndrome outbreak on genetic parameters and reaction norms for reproductive performance in pigs1

Schmitz‐Esser

Gray³

et al. 2020

Sci Rep

previous studies have demonstrated evidence for a relationship between the vaginal microbiome and reproductive performance, suggesting the vaginal microbiota may serve as a tool to predict farrowing outcomes in commercial pigs. in this study, we compared the vaginal microbiome in sows with low and high farrowing performance and used it to classify animals with contrasting reproductive outcomes in commercial sows following immune challenge with porcine respiratory and reproductive syndrome (PRRS) vaccination. Eighteen microbes were differentially abundant (q-value < 0.05) between the Low and High farrowing performance groups. Among them, Campylobacter, Bacteroides, Porphyromonas, Lachnospiraceae unclassified, Prevotella, and Phascolarctobacterium were also selected in the discriminant and linear regression analyses, and could be used as potential biomarkers for reproductive outcomes. The correct classification rate in the two groups was 100%. In conclusion, this study demonstrates that vaginal microbiota collected after pRRS vaccination could be potentially used to classify sows into having low or high farrowing performance in commercial herds.

Section: Discussionmentioning

confidence: 99%

Vaginal microbiota diverges in sows with low and high reproductive performance after porcine reproductive and respiratory syndrome vaccination

Schmitz‐Esser

Gray³

et al. 2020

Sci Rep

“…These authors measured S/P ratio at about 46 days after the PRRS outbreak using the same ELISA test utilized in our study. Also using purebred pregnant sows under a PRRS outbreak, Putz et al (2019) reported a much lower h 2 estimate, of 0.17 ± 0.05 during the PRRS outbreak. In their study, antibody response to PRRSV was measured at about 60 days after the PRRS outbreak.…”

Section: Heritabilitiesmentioning

confidence: 91%

“…In their study, antibody response to PRRSV was measured at about 60 days after the PRRS outbreak. There were three main differences between Serão et al (2014) and Putz et al (2019) that could be associated with different estimates of h 2 observed. First, the time of sample collection in Putz et al (2019) was of about 2 weeks after the PRRS outbreak than in Serão et al (2014), which could indicate a different immunological response of the animals (further discussion below).…”

Section: Heritabilitiesmentioning

confidence: 97%

“…In addition, these authors showed that S/P ratio was highly genetically favorably correlated with litter size traits during PRRSV infection, such as number born alive (NBA), with an estimate of 0.73 and number of stillborn (NSB) of -0.72. Putz et al (2019) also reported a high and negative r g estimate between S/P ratio and NSB (-0.73). Nonetheless, these authors obtained a weak r g estimate between S/P and NBA (0.05) and a low h 2 estimate for S/P ratio (0.17).…”

Section: Introductionmentioning

confidence: 94%

“…However, Hickmann et al (2019) reported moderate h 2 estimates of S/P in Duroc (0.33 ± 0.06) and Landrace (0.28 ± 0.07) sow populations during a PRRS outbreak, measured about 54 days after the outbreak using the same ELISA test used in our study. As in Putz et al (2019), these animals were re-exposed to the PRRSV prior to collection of samples to measure antibody response. Therefore, low h 2 estimate found in Putz et al (2019) for S/P ratio is likely associated with the differences in diagnostic assays.…”

Section: Heritabilitiesmentioning

confidence: 99%

See 2 more Smart Citations

Genetic Analysis of Antibody Response to Porcine Reproductive and Respiratory Syndrome Vaccination as an Indicator Trait for Reproductive Performance in Commercial Sows

Fernando

Gray³

et al. 2020

Front. Genet.

We proposed to investigate the genomic basis of antibody response to porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) vaccination and its relationship to reproductive performance in non-PRRSV-infected commercial sows. Nine hundred and six F1 replacement gilts (139 ± 17 days old) from two commercial farms were vaccinated with a commercial modified live PRRSV vaccine. Blood samples were collected about 52 days after vaccination to measure antibody response to PRRSV as sample-to-positive (S/P) ratio and for single-nucleotide polymorphism (SNP) genotyping. Reproductive performance was recorded for up to 807 sows for number born alive (NBA), number of piglets weaned, number born mummified (MUM), number of stillborn (NSB), and number of pre-weaning mortality (PWM) at parities (P) 1-3 and per sow per year (PSY). Fertility traits such as farrowing rate and age at first service were also analyzed. BayesC0 was used to estimate heritability and genetic correlations of S/P ratio with reproductive performance. Genome-wide association study (GWAS) and genomic prediction were performed using BayesB. The heritability estimate of S/P ratio was 0.34 ± 0.05. High genetic correlations (r g) of S/P ratio with farrowing performance were identified for NBA P1 (0.61), PWM P2 (-0.70), NSB P3 (-0.83), MUM P3 (-0.84), and NSB PSY (-0.90), indicating that genetic selection for increased S/P ratio would result in improved performance of these traits. A quantitative trait locus was identified on chromosome 7 (∼25 Mb), at the major histocompatibility complex (MHC) region, explaining ∼30% of the genetic variance for S/P ratio, mainly by SNPs ASGA0032113, H3GA0020505, and M1GA0009777. This same region was identified in the bivariate GWAS of S/P ratio and reproductive traits, with SNP H3GA0020505 explaining up to 10% (for NBA P1) of the genetic variance of reproductive performance. The heterozygote genotype at H3GA0020505 was associated with greater S/P ratio and NBA P1 (P = 0.06), and lower MUM P3 and NSB P3 (P = 0.07). Genomic prediction

Genomics of response to porcine reproductive and respiratory syndrome virus in purebred and crossbred sows: antibody response and performance following natural infection vs. vaccination

Journal of Animal Science

Hickmann

Huang³

et al. 2021

Self Cite

Antibody response, measured as sample-to-positive (S/P) ratio, to Porcine Reproductive and Respiratory Syndrome virus (PRRSV) following a PRRSV-outbreak (S/POutbreak) in a purebred nucleus and following a PRRSV-vaccination (S/PVx) in commercial crossbred herds have been proposed as genetic indicator traits for improved reproductive performance in PRRSV-infected purebred and PRRSV-vaccinated crossbred sows, respectively. In this study, we investigated the genetic relationships of S/POutbreak and S/PVx with performance at the commercial (vaccinated crossbred sows) and nucleus level (non-infected and PRRSV-infected purebred sows), respectively, and tested the effect of previously identified SNP for these indicator traits. Antibody response was measured on 541 Landrace sows approximately 54 days after the start of a PRRSV outbreak, and on 906 F1 (Landrace x Large White) gilts approximately 50 days after vaccination with a commercial PRRSV vaccine. Reproductive performance was recorded for 711 and 428 Landrace sows before and during the PRRSV outbreak, respectively, and for 811 vaccinated F1 animals. The estimate of the genetic correlation (rg) of S/POutbreak with S/PVx was 0.72±0.18. The estimates of rg of S/POutbreak with reproductive performance in vaccinated crossbred sows were low to moderate, ranging from 0.05±0.23 to 0.30±0.20. The estimate of rg of S/PVx with reproductive performance in non-infected purebred sows was moderate and favorable with number born alive (0.50±0.23) but low (0±0.23 to -0.11±0.23) with piglet mortality traits. The estimates of rg of S/PVx were moderate and negative (-0.38±0.21) with number of mummies in PRRSV-infected purebred sows and low with other traits (-0.30±0.18 to 0.05±0.18). Several significant associations (P0 > 0.90) of previously reported SNP for S/P ratio (ASGA0032063 and H3GA0020505) were identified for S/P ratio and performance in non-infected purebred and PRRSV-exposed purebred and crossbred sows. Genomic regions harboring the major histocompatibility complex class II region significantly contributed to the genetic correlation of antibody response to PRRSV with most of the traits analyzed. These results indicate that selection for antibody response in purebred sows following a PRRSV outbreak in the nucleus and for antibody response to PRRSV vaccination measured in commercial crossbred sows are expected to increase litter size in purebred and commercial sows.