The effect of Acacia nilotica bark extract on growth performance, carcass characteristics, immune response, and intestinal morphology in broilers as an alternative to antibiotic growth promoter

Zahid, Muhammad Umer; Khalique, Anjum; Qaisrani, Shafqat Nawaz; Ashraf, Muhammad; Sheikh, Ali Ahmad; Yaqoob, Muhammad Umar

doi:10.5713/ab.22.0284

Cited by 2 publications

(2 citation statements)

References 31 publications

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“…The growth performance of animals is closely related to their intestinal morphology (Yousefi et al, 2023;Zahid et al, 2023). The VH, CD and VH/CD are important indicators for measuring intestinal digestion and absorption function (Gu et al, 2022;Martel et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Bile acids supplementation modulates lipid metabolism, intestinal function, and cecal microbiota in geese

Wang

Liu

et al. 2023

Front. Microbiol.

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Bile acids(BAs) are important components of bile and play a significant role in fat metabolism. However, there is currently no systematic evaluation of the use of BAs as feed additives for geese.This study aimed to investigate the effects of adding BAs to goose feed on growth performance, lipid metabolism, intestinal morphology, mucosal barrier function, and cecal microbiota. A total of 168 28-day-old geese were randomly assigned to four treatment groups and fed diets supplemented with 0, 75, 150, or 300 mg/kg of BAs for 28 days. The addition of 75 and 150 mg/kg of BAs significantly improved the feed/gain (F/G) (p < 0.05).The addition of BAs decreased abdominal fat percentage and serum total cholesterol (TC) levels, with 150 mg/kg of BAs significantly reducing serum triglyceride levels and increased expression of Farnesoid X Receptor (FXR) mRNA in the liver(p < 0.05), 300 mg/kg of BAs significantly increasing the expression level of liver peroxisome proliferator-activated receptor α (PPARα) (p < 0.05). In terms of intestinal morphology and mucosal barrier function, 150 mg/kg of BAs significantly increased villus height (VH) and VH/crypt depth (CD) in the jejunum (p < 0.05). The addition of 150 and 300 mg/kg of BAs significantly reduced the CD in the ileum, while increasing VH and VH/CD (p<0.05). Additionally, the addition of 150 and 300 mg/kg of BAs significantly increased the expression levels of zonula occludens-1 (ZO-1) and occludin in the jejunum. Simultaneously 150mg/kg and 300mg/kg BAs increased the total short-chain fatty acids (SCFA) concentrations in the jejunum and cecum(p < 0.05).Supplementation with BAs resulted in a significant increase in the ɑ-diversity of cecal microbiota and a decrease in the abundance of Proteobacteria in the cecum. The addition of 150 mg/kg of BAs significantly reduced the abundance of Bacteroidetes and increased the abundance of Firmicutes. Moreover,Linear discriminant analysis Effect Size analysis (LEfSe) showed that the abundances of bacteria producing SCFA and bile salt hydrolases (BSH) were increased in the BAs-treated group. Furthermore, Spearman’s analysis showed that the genus Balutia, which is negatively correlated with visceral fat area, was positively correlated with serum high-density lipoprotein cholesterol (HDL-C), while Clostridium was positively correlated with intestinal VH and VH/CD. In conclusion, BAs can be considered an effective feed additive for geese, as they increased SCFA concentration, improve lipid metabolism and intestinal health by enhancing the intestinal mucosal barrier, improving intestinal morphology, and altering the cecal microbiota structure.

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Section: Discussionmentioning

confidence: 99%

Bile acids supplementation modulates lipid metabolism, intestinal function, and cecal microbiota in geese

Wang

Liu

et al. 2023

Front. Microbiol.

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“…A. nilotica is a tree with diverse potential anti-cancer and anti-toxic abilities [1] and has been reported to have different medicinal uses and various pharmacological properties. [2][3][4][5] Ranging from anti-bacterial, [6,7] anti-viral, anti-oxidant, [8] anti-diabetic, anti-mutagenic, and cytotoxic activities. [9] Different chemical components were found in A. nilotica from different parts with potential for different anti-cancer and anti-toxic actions.…”

Section: Introductionmentioning

confidence: 99%

Acacia nilotica Pod Extract has an Anti-cancer Effect on the U937 Cell Line

Bakheit,

Taurin,

Mohamed Elamin

et al. 2023

Pharmacogn. Res.

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Background: Acacia nilotica (A. nilotica), a tree that thrives in tropical regions, has been reported to have various medicinal uses and various pharmacological properties. Potential anti-cancer effects are one of the medical properties of parts of this tree. Objectives: To explore and evaluate the cytotoxic effect of A. nilotica, we used Acacia nilotica's pod extract on U937 cell lines as an acute myeloid leukemia model. Materials and Methods: Microscopic analysis, flow cytometry, and WST-1 tests were performed on the U937 cell line after 72 hr of treatment with different concentrations of ethanol pod extract from Acacia nilotica (A. nilotica) to test for DNA cell cycle, cell viability and apoptosis, and to determine the IC 50 of the drug. Results: Treatment with A. nilotica pod extract significantly induced cell death in U937-treated cells compared with untreated control cells. Microscopic analysis revealed morphological changes in dead and fragmented cells associated with a reduction in cell number. A reduction in cell viability with an increase of cells in the late apoptosis stage by flow cytometry indicated the cytotoxic effect of A. nilotica and accumulation of cells in the sub-G1 phase of the DNA cell cycle analysis. The IC 50 was identified by the WST-1 assay. Conclusion: The findings of the present study suggest that A. nilotica may be employed as a natural anti-cancer agent in research on acute myeloid leukemia in humans.

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The effect of Acacia nilotica bark extract on growth performance, carcass characteristics, immune response, and intestinal morphology in broilers as an alternative to antibiotic growth promoter

Cited by 2 publications

References 31 publications

Bile acids supplementation modulates lipid metabolism, intestinal function, and cecal microbiota in geese

Bile acids supplementation modulates lipid metabolism, intestinal function, and cecal microbiota in geese

Acacia nilotica Pod Extract has an Anti-cancer Effect on the U937 Cell Line

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