“…The effect of cycloheximide (actidione) on cell wall synthesis in protoplasts of S. cerevisiae is interesting. The formation of a fibrillar network was not affected by cycloheximide in liquid media (82), whereas growth of protoplasts and protein synthesis were inhibited immediately (96). Fibrils formed even when the yeast cells were treated with cycloheximide 2 to 4 hr before their conversion to protoplasts.…”