The research objective was to determine how the feeding diet made of microparticle protein combined with prebiotic sources affected broilers’ intestinal bacteria and growth performance. Micronized fish meals and soybean meals were produced. Inulin, glucomannan, and soybean oligosaccharide were obtained from extracts of the dahlia tuber (DTE), porang tuber (PTE), and soybean meal (SME), respectively. A completely randomized design with four treatments (D1: 21% intact protein (control), D2: 18% microparticle protein + 1.2% DTE, D3: 18% microparticle protein + 0.3% PTE, and D4: 18% microparticle protein + 0.2% SME) and six replication (8 birds each) was assigned. Protein digestibility, H/L ratio, growth performance, lactic acid bacteria (LAB), E. coli, and other variables were exposed to ANOVA and the Duncan test with a 5% probability. Except for FCR, all parameters were substantially (p0.05) higher when prebiotic sources were included in the diet compared to the control group (D1). However, D3‘s LAB population and H/L ratio were the same compared to other treatments, and feed consumption was the same across all treatments. Supplementing a diet with microparticle protein with inulin from dahlia tubers and soy-oligosaccharide of soybean meal extracts improves growth performance, which is supported by an increase in LAB population and more easily digestible protein with a lower H/L ratio