The  Effect of Aerobic and Anaerobic Interval Exercise on the Proliferation Phase of Wound Healing in Tooth Extraction of Rattus novergicus

Background: Physical exercise has been proven to accelerate wound healing. Physical training itself consists of aerobic (continuous training) and anaerobic (interval training) exercise. The effectiveness of continuous physical exercise on post-tooth extraction wound healing is the focus of this study. Purpose: This study aims to investigate the differences in post-tooth extraction wound healing in Wistar rats (Rattus norvegicus) after aerobic and anaerobic exercise based on the number of fibroblasts and neovascularisation. Methods: Wistar rats were divided into three groups: the control group (K1); K2 undertook continuous aerobic exercise, swimming at 50% maximum swimming capacity (MSC) with an additional 3% bodyweight load; K3 undertook anaerobic continuous exercise, swimming at 65% MSC with a 6% load. The rats swam three times per week for six weeks. The number of fibroblasts and neovascularisation were examined three days after tooth extraction. Data was analysed using the one-way analysis of variance (ANOVA) and Least Significant Difference (LSD) tests (p<0.05). Results: There was a significant difference in the number of fibroblasts between the K2 and K3 groups. There was no significant difference between K2 and K3 in the amount of neovascularisation. Conclusion: There were differences in the number of fibroblasts but not neovascularisation after tooth extraction in Wistar rats given aerobic and anaerobic continuous training.

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Proliferation of Mesenchymal Stem Cells in Carbonate Apatite-Chitosan Scaffolds in Bone Tissue Engineering Techniques

Oki¹,

Ariani²

2019

Cumhuriyet Dental Journal

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Objectives: Chitosan is a popular bone graft material. However, chitosan also has a weakness for cell adhesion and the lack of sufficient bone formation capabilities. To improve it, we tried to develop a chitosan scaffolds that combine with carbonate apatite (CA), which has excellent biocompatibility properties of the tissues of the human body. We tried to find the most appropriate amount of Carbon Apatite (CA) to be combined with Chitosan scaffold (ChSs) to produce a good scaffolds structure, as well as to evaluate CA-ChSs from the standpoint of cell proliferation using mesenchymal stem cells (MSCs). Materials and Methods: Porous chitosan matrix was made by using the lyophilization technique. ChSs containing chitosan powder was made by the following procedure. 100, 150, 200, and 400 mg of chitosan powder was dissolved in 5 ml of 2% acetic acid at room temperature. ChSs containing 200 mg of chitosan powder was chosen to make CA-ChSs. Ultraviolet radiation was then performed for 2 hours. CA-ChSs structure was observed by a scanning electron microscope (SEM). Proliferation of MSCs in CA-ChSs evaluated at days 1, 3, 5 and 7.Results: This study demonstrated that CA-ChSs containing 200 mg of chitosan powder and 50 mg CA has a three-dimensional structure that is porous and attachment powder CA in the pores and absorbance values were increased from the examination day 1 until day 7. Cell proliferation using MSCs in CA-ChSs are better, as the absorbance value of CA-Chss with 50 mg CA content was significantly higher than ChSs. These findings also confirm that MSCs has good viability and biocompatibility in bone tissue engineering techniques. Conclusions:Based on these results, it is expected CA-ChSs may be candidates for bone graft material in tissue engineering techniques.

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The Effect of Aerobic and Anaerobic Interval Exercise on the Proliferation Phase of Wound Healing in Tooth Extraction of Rattus novergicus

Cited by 3 publications

References 16 publications

Synthesis of Silver Nanoparticles from Lemongrass Leaves Induced Wound Healing by Reduction ROS Fibroblasts

Synthesis of Silver Nanoparticles from Lemongrass Leaves Induced Wound Healing by Reduction ROS Fibroblasts

Acceleration of post-tooth extraction socket healing after continuous aerobic and anaerobic physical exercise in Wistar rats (Rattus norvegicus)

Proliferation of Mesenchymal Stem Cells in Carbonate Apatite-Chitosan Scaffolds in Bone Tissue Engineering Techniques

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