The effect of age and length of gonadotropin stimulation on the in vitro embryo development of Holstein calf oocytes

Currin, Luke; Michalovic, Laura; Bellefleur, Anne‐Marie; Gutierrez, Karina; Glanzner, Werner Giehl; Schuermann, Yasmin; Bohrer, Rodrigo Camponogara; Dicks, Naomi; Rosa, Paulo Ricardo da Silva; Cesaro, Matheus Pedrotti de; Lopez, Rosalba; Grand, François‐Xavier; Vigneault, Christian; Blondin, Patrick; Gourdon, Jim C.; Baldassarre, Hernán; Bordignon, Vilceu

doi:10.1016/j.theriogenology.2017.08.011

Cited by 40 publications

(46 citation statements)

References 26 publications

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“…Hence, it is extremely important to provide a proper exogenous gonadotropin regime that will support follicular development and oocyte acquisition of developmental competence. In agreement with this concept, our work with Holstein calves of 2-6 months of age, showed higher rates of development to the blastocyst stage when oocytes were sourced from calves subjected to longer gonadotropin stimulation (3 days) compared with short (2 days) or non-stimulated, which was associated with higher proportion of larger follicles (Table 1; Currin et al, 2017). Moreover, embryo development was significantly higher with oocytes recovered from larger (>5 mm) follicles (21%) compared with smaller follicles (11%, P < 0.05).…”

Section: Introductionsupporting

confidence: 78%

“…In that sense, LOPU rapidly became, and remains as, the method of choice for the collection of immature oocytes for IVEP in sheep and goats (Baldassarre et al, 1994;Stangl et al, 1999;Baldassarre et al, 2002;Cognie et al, 2004). Moreover, LOPU-IVEP has also been applied for oocyte collection in wild life species including deer and big felines (Miller et al, 1990;Locatelli et al, 2006;Baldassarre et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…In general, this seems to be the common procedure from what is found in the literature. Indeed, most articles show littleto-no deviations in the procedures respect of those in use for oocytes from adult cows and heifers (Revel et al, 1995;Armstrong et al, 1997;Khatir et al, 1998;Majerus et al, 2000;Taneja et al, 2000;Kauffold et al, 2005b;Currin et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Baldassarre

Bordignon

2018

Anim Reprod

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Laparoscopic ovum pick-up (LOPU) conducted on bovine and buffalo calves of 2-6-month of age, followed by in vitro embryo production and transfer into synchronous adult recipients, is a powerful tool for accelerated genetic gain and early dissemination of top genetics. In its current state, the technology is characterized by higher oocyte recovery rates, lower oocyte-to-embryo yields, and similar pregnancy and term development rates compared with adult counterparts. Improvements in oocyte competence have been made in recent years mainly through gonadotropin stimulation protocols tailored for prepubertal donors. These advances have brought the technology to the point of been apt for commercial application. However, future research must focus on increasing the proportion of fully competent oocytes recovered from calves thereby further empowering the role this technology platform can play in programs for accelerated dissemination of superior genetics.

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Section: Introductionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Baldassarre

Bordignon

2018

Anim Reprod

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“…They found that young (5-10 months) Holstein females had a more pronounced response to FSH stimulation, as evidenced by a larger number of small (5-6 mm) and medium (7-10 mm) follicles, compared with postpubertal females aged 16-18 months. However, oocytes obtained from younger donors lacked full meiotic and/or developmental competence, demonstrated by a lower blastocyst rate (Landry et al 2016), a phenomenon also observed by (Currin et al 2017). Studies in ruminants are conflicting as to whether DNA methylation is different between pre-and postpubertal animals.…”

Section: The Oocytementioning

confidence: 99%

Consequences of assisted reproductive techniques on the embryonic epigenome in cattle

Rivera¹

2020

Reprod. Fertil. Dev.

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Procedures used in assisted reproduction have been under constant scrutiny since their inception with the goal of improving the number and quality of embryos produced. However, in vitro production of embryos is not without complications because many fertilised oocytes fail to become blastocysts, and even those that do often differ in the genetic output compared with their in vivo counterparts. Thus only a portion of those transferred complete normal fetal development. An unwanted consequence of bovine assisted reproductive technology (ART) is the induction of a syndrome characterised by fetal overgrowth and placental abnormalities, namely large offspring syndrome; a condition associated with inappropriate control of the epigenome. Epigenetics is the study of chromatin and its effects on genetic output. Establishment and maintenance of epigenetic marks during gametogenesis and embryogenesis is imperative for the maintenance of cell identity and function. ARTs are implemented during times of vast epigenetic reprogramming; as a result, many studies have identified ART-induced deviations in epigenetic regulation in mammalian gametes and embryos. This review describes the various layers of epigenetic regulation and discusses findings pertaining to the effects of ART on the epigenome of bovine gametes and the preimplantation embryo.

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“…taurus and B. indicus aging from 2 to 4 months did not exhibit an improvement in IVF results when stimulated with 140 mg of FSH (Batista et al, 2016). However, recently, more prolonged FSH stimulation (three days) was shown to increase the development competence of Holstein calf oocytes, which was associated with a higher proportion of follicles larger than 5 mm (Currin et al, 2017).…”

Section: Collection and Quality Of Oocytes From Prepubertal Heifers Amentioning

confidence: 99%

Intensive use of IVF by large-scale dairy programs

Sanches¹,

Zangirólamo

Seneda

2019

Anim Reprod

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The number of embryos produced by in vitro fertilization (IVF) has grown exponentially in recent years. Recently, for the first time, the number of embryos produced and transferred in vitro was significantly higher than the number developed in vivo worldwide. In this context, a particular boost occurred with ovum pick-up (OPU) and in vitro embryos produced in North America, and this technology is becoming more prominent for commercial dairy farms. However, despite many advances in recent decades, laboratories and companies are looking for methods and alternatives that can be used in collaboration with the existing process to improve it. Among the strategies used to improve the dairy industry are the use of genomic analysis for the selection of animals with desired traits or as an evaluation tool of oocyte and embryo quality, the optimization of the collection and use of gametes from prepubertal females and males, the effective use of sexed semen, and improvements in culture media and methods of embryo cryopreservation. Thus, this review aims to discuss the highlights of the commercial use of IVF and some strategies to increase the application of this technique in large-scale dairy programs.

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The effect of age and length of gonadotropin stimulation on the in vitro embryo development of Holstein calf oocytes

Cited by 40 publications

References 26 publications

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Consequences of assisted reproductive techniques on the embryonic epigenome in cattle

Intensive use of IVF by large-scale dairy programs

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