The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes

Fenech, Michael; Bonassi, Stefano

doi:10.1093/mutage/geq050

Cited by 318 publications

(203 citation statements)

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“…Several studies found that increase of MN frequencies with an increasing age was followed by a decrease in proliferating efficiencies of cells [20][21][22]. The increase of MN frequencies with age can be due to several factors likes the cumulative effect of acquired mutations in genes involved in DNA repair, the numerical and structural aberrations in chromosomes caused by exposure to endogenous genotoxins, inadequate nutrition, exposure to environmental or occupational genotoxins, as well as a wide range of unhealthy lifestyle factors [23]. In this study we also found a higher of BI in female compared to male in all samples (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Peripheral Blood Lymphocytes Proliferation in Botteng Village, Mamuju Inhabitants Using Binucleate Index

Ramadhani

Sardini

Masnelli

et al. 2017

J. Ilmiah Aplikasi Isotop dan Radiasi

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Botteng Village in Mamuju, West Sulawesi was known for the high natural background radiation exposure. Botteng Village inhabitants exposed to high natural radiation in their daily life. Radiation exposure can inhibit the mitosis mechanism at various phases. Our previous study revealed that mitotic and nuclear division indexes in Botteng Village inhabitants were lower compared to control samples. To validate our previous study results here we evaluate the binucleate index in peripheral blood lymphocytes of Botteng Village inhabitants. Blood samples were collected from thirteen healthy adult subjects in Botteng Village and thirteen healthy adult subjects in normal background radiation area. Binucleate index was calculated as the proportion of binucleated cell (BNC) in 500 cells for each sample. The study result showed that the BI in Botteng Village was similar compared to control group (23.58 ± 9.60 vs 23.47 ± 6.24). It is possible that the small sample numbers used in this study were not adequate to represent the BI value in Botteng Village inhabitants. This study also showed that there was insignificant difference of BI in respect to gender and age for all samples. Further study using larger sample number should be conducted to verify whether the BI can be used as a marker of lymphocytes proliferation.Keywords : Binucleated cells, Binucleate index, Botteng, Cell proliferation, Natural radiation ABSTRAK Desa Botteng di Mamuju, Sulawesi Barat diketahui memiliki paparan radiasi latar dari alam yang cukup tinggi. Penduduk desa Botteng menerima paparan radiasi alam tinggi dalam kehidupan sehariharinya. Paparan radiasi dapat menghambat proses mitosis dalam berbagai tahapan. Studi yang telah dilakukan menunjukkan bahwa indeks mitosis dan indeks pembelahan inti pada penduduk desa Botteng lebih rendah dibandingkan dengan sampel kontrol. Untuk memvalidasi hasil studi tersebut dilakukan evaluasi terhadap indeks binukleus di limfosit darah tepi penduduk desa Botteng. Sampel darah diperoleh dari tiga belas penduduk dewasa yang sehat di desa Botteng Desa dan daerah dengan radiasi latar normal. Indeks binukleus dihitung sebagai proporsi sel binukleus dalam 500 total sel dari setiap sampel. Hasil studi memperlihatkan bahwa nilai indeks binukleus pada penduduk desa Botteng hampir sama bila dibandingkan dengan kelompok kontrol (23,58 ± 9,60 dan 23,47 ± 6,24). Terdapat kemungkinan bahwa jumlah sampel yang sedikit dalam penelitian yang dilakukan tidak dapat merepresentasikan nilai indeks binukleus di penduduk desa Botteng. Hasil studi memperlihatkan bahwa tidak terdapat perbedaan yang signifikan pada nilai indeks binukleus terkait dengan jenis kelamin dan umur pada semua sampel. Studi lebih lanjut dengan menggunakan jumlah sampel yang lebih besar harus dilakukan untuk memastikan apakah nilai indeks binukleus dapat digunakan sebagai penanda proses pembelahan sel limfosit.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Peripheral Blood Lymphocytes Proliferation in Botteng Village, Mamuju Inhabitants Using Binucleate Index

Ramadhani

Sardini

Masnelli

et al. 2017

J. Ilmiah Aplikasi Isotop dan Radiasi

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“…MN is expressed in cells when either acentric chromosome fragments or whole chromosomes fail to be segregated to the daughter nuclei during mitosis 9) . It also seems that the CBMN assay can be used to measure not only whole chromosome loss or chromosome breaks but also excision-repair events 8,22) .…”

Section: Discussionmentioning

confidence: 99%

“…These all contributed to the overarching uncertainty surrounding the dose-response model and the final estimates. Third, unlike hepatic angiosarcoma, MN remains a nonspecific disease marker, and other confounding factors may influence it, such as age, gender, diet, lifestyle factors, disease and genetic factors 9) . For example, our study did not have quantitative data about smoking habits, and in the absence of such data, the simple comparison of smokers versus nonsmokers might be misleading.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to mutations, other genotoxic effects were reported such as gene conversion, sister chromatid exchanges (SCE), micronuclei (MN), mitotic recombination, chromosomal aberrations (CA) and cell transformation 3,6,7) . The MN assay has become a well-known and promising indicator to investigate the genotoxicity of a variety of chemicals especially using the cytokinesis-block micronucleus (CBMN) assay 8,9) . The CBMN method The aim of this study was to estimate a benchmark dose (BMD) for chromosome damage induced by vinyl chloride monomer (VCM) in VCMexposed workers in central China and validate the published results in Shanghai.…”

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confidence: 99%

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Estimation of a Safe Level for Occupational Exposure to Vinyl Chloride Using a Benchmark Dose Method in Central China

Jiao

Feng

et al. 2012

Journal of Occupational Health

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Estimation of a Safe Level for Occupational Exposure to Vinyl Chloride Using a Benchmark Dose Method in Central China: Jie JIAO, et al. Department of Occupational Health and Toxicology, School of Public Health, Fudan University, China— Objectives The aim of this study was to estimate a benchmark dose (BMD) for chromosome damage induced by vinyl chloride monomer (VCM) in VCMexposed workers in central China and validate the published results in Shanghai. Methods VCMexposed workers who had been exposed to VCM for at least one year (n=463) and matched subjects not exposed to VCM or other toxins (n=273) were asked to participate in this study. Micronucleus (MN) frequency based on the cytokinesis‐block micronucleus assay (CBMN) was used as a biomarker for chromosome damage induced by VCM exposure. Results The MN frequency in the VCM‐exposed workers was significantly higher than that in the control group, and multivariate Poisson regression suggested that gender, smoking status and VCM exposure were the significant factors influencing the risk of increased MN frequency. When subjects were further stratified according to gender and smoking status, the results showed that female VCMexposed workers were more susceptible than the males to the risk of increased MN frequency. The MN frequency of smokers was significantly higher than that of nonsmokers in the control group. Our study also suggested that there was a strong dose‐response relationship between VCM CED and the increased risk of MN frequency in the total group, males and females. The BMDL10 was found to be 630.6, 670.2 and 273.7 mgyear for all VCM‐exposed workers, males and females, respectively. Conclusions These results invite further scrutiny of the current VCM occupational exposure limits and warrant further study of the risk of VCM genotoxicity and carcinogenicity.

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“…MN can be formed from acentric chromosomal fragments or whole chromosomes fail to be segregated to the daughter nuclei during mitotic cellular division (Fenech and Bonassi 2011). Thus, these genotoxicity tests are well-established markers for the determination of the genotoxic effects of compounds.…”

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confidence: 99%

The effects of 4-thujanol on chromosome aberrations, sister chromatid exchanges and micronucleus in human peripheral blood lymphocytes

Kocaman¹,

Rencüzoğulları²,

Topaktaş³

et al. 2011

Cytotechnology

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4-Thujanol, a bicyclic monoterpene alcohol, is present in the essential oils of many medicinal and aromatic plants. It is commonly used as a fragrance and flavouring ingredient in a lot of different products. The potential genotoxic effects of 4-thujanol on human peripheral blood lymphocytes (PBLs) were investigated in vitro by the chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and micronucleus (MN) tests. The cells were treated with 13, 26 and 52 lg/mL 4-thujanol in the presence and absence of a metabolic activator (S9 mix). 4-Thujanol induced CA (P \ 0.001) and MN formation (P \ 0.05) at all concentrations (13, 26 and 52 lg/mL) in the presence and absence of the S9 mix without a concentration-dependent manner. However, the treatment of peripheral lymphocytes with 4-thujanol did not produce a statistical difference in the frequency of SCEs when compared with control group. Furthermore, this monoterpene did not significantly decrease the mitotic index (MI), proliferation index (PI), and nuclear division index (NDI). In conclusion, 4-thujanol had a significant clastogenic effect at the tested concentrations (13, 26 and 52 lg/mL) for human PBLs. In addition, no cytotoxic and/or cytostatic effects were observed regardless of the concentrations used. This work presents the first report on genotoxic properties of 4-thujanol.

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The effect of age, gender, diet and lifestyle on DNA damage measured using micronucleus frequency in human peripheral blood lymphocytes

Cited by 318 publications

References 82 publications

Evaluation of Peripheral Blood Lymphocytes Proliferation in Botteng Village, Mamuju Inhabitants Using Binucleate Index

Evaluation of Peripheral Blood Lymphocytes Proliferation in Botteng Village, Mamuju Inhabitants Using Binucleate Index

Estimation of a Safe Level for Occupational Exposure to Vinyl Chloride Using a Benchmark Dose Method in Central China

The effects of 4-thujanol on chromosome aberrations, sister chromatid exchanges and micronucleus in human peripheral blood lymphocytes

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