The Effect of Age on Proliferating Cell Nuclear Antigen Expression in Oral Gingival Epithelium of Healthy and Inflamed Human Gingiva

Çelenligil-Nazliel, Haviye; Ayhan, Ayşe; Uzun, Hakan; Ruacan, Şevket

doi:10.1902/jop.2000.71.10.1567

Cited by 30 publications

(36 citation statements)

References 45 publications

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“…In this study, we did not find any significantly changed level of PCNA expression in the gingival epithelia of the PED group. In contrast with our findings, some studies suggest the presence of increased PCNA activity in oral gingival epithelium during inflammation (38,41) and Tsuji et al suggested a higher level of PCNA protein in tumors (42). Thus, it becomes apparent that ALA can affect PCNA activity and/ or apoptosis related pathologic conditions in the gingival tissue of periodontitis induced rats.…”

Section: Discussioncontrasting

confidence: 99%

“…In a normal cell cycle, PCNA is a nuclear protein that has an important function in DNA synthesis. PCNA protein level is directly related to cell proliferation and PCNA functions are controlled by p21 CIF1 ⁄ WAF1 , which is also controlled by the p53 gene (33,38,39). The P53 gene contributes to the inhibition of cell growth and can actively arrest the cell cycle and trigger apoptosis (40).…”

Section: Discussionmentioning

confidence: 99%

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Influence of alpha lipoic acid on epithelial apoptosis in experimental periodontitis

Kara¹,

Akman²,

DEMİRCİ³

et al. 2013

Turk J Med Sci

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In this work, direct and indirect methods for determination of vitamin K 3 were developed using differential pulse polarography. The reaction between sulfite and the quinone (Q) form of vitamin K 3 was used for indirect determination, since the sulfite peak at -0.7 V is sharp and very reproducible in 0.1 M HAc NaAc (pH = 5.5). It was found that at pH 4.5-5.5, this reaction was quantitative and very fast when the temperature was 45 • C. For its direct determination, on the other hand, vitamin K 3 was standardized by the indirect method using standard sulfite as the reducing agent. The calibration graph for vitamin K 3 (in Q form), using the peak at -1.0 V in a HAc-NaAc medium with a pH of 5.5, was linear for concentrations ranging from 5 × 10 −7 to 3 × 10 −5 M, and the limit of detection (LOD) was 1.5 × 10 −7 M. The proposed methods were successfully applied to the determination of vitamin K 3 in a clinical injection solution and in blood serum.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of alpha lipoic acid on epithelial apoptosis in experimental periodontitis

Kara¹,

Akman²,

DEMİRCİ³

et al. 2013

Turk J Med Sci

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“…3 H]-thymidine (15,16) and by immunohistochemical detection of markers such as proliferating cell nuclear antigen (PCNA) (17). A few junctional epithelial cells attached to tooth were labeled by incubation for three hours in medium containing [ In contrast, the sulcular and oral gingival epithelia exhibited certain level of proliferative activity as shown in Table 1.…”

Section: Discussionmentioning

confidence: 99%

Localization of bromodeoxyuridine-incorporating, p63- and p75NGFR- expressing cells in the human gingival epithelium

et al. 2007

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“…Nevertheless, there is no consensus in the literature on how epithelial maturation and proliferation are affected by age 6,13,15,19,24) . Çelenligil-Nazliel et al 4) did not find differences in epithelial renewal. However, aging is related to a gradual reduction in connective tissue turnover.…”

Section: Introductionmentioning

confidence: 87%

Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

Carrard

Pires

Badauy

et al. 2008

Bull. Tokyo Dent. Coll.

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The aim of this study was to investigate cell proliferation rate and certain morphological features of mouse epithelium as aging progresses. Tongue biopsies were performed on female mice (Mus domesticus domesticus) at 2, 8, 14 and 20 months of age as indicative of adolescence, adulthood, early senescence and senescence, respectively. Histological sections of tongue were stained with hematoxylin-eosin and subjected to silver staining for active nucleolar organizer region counting. Cell proliferation rate and epithelial thickness analysis were carried out. Analysis of variance detected no differences between the groups in terms of numbers of silver-stained dots associated with nucleolar proteins. There was an increase in mean epithelial thickness in adult animals, followed by a gradual reduction until senescence. Mean keratin thickness presented an increase at 8 and 20 months of age. This difference is probably related to puberty, growth or dietary habits. Aging has no influence on oral epithelial proliferation rate in mice. A gradual reduction in epithelial thickness is a feature of aging in mammals. A conspicuous increase in the keratin layer was observed in senescence as an adaptative response to the reduction in epithelial thickness. These results suggest that aging affects the oral epithelium maturation process through a mechanism that is not related to cell proliferation.

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The Effect of Age on Proliferating Cell Nuclear Antigen Expression in Oral Gingival Epithelium of Healthy and Inflamed Human Gingiva

Cited by 30 publications

References 45 publications

Influence of alpha lipoic acid on epithelial apoptosis in experimental periodontitis

Influence of alpha lipoic acid on epithelial apoptosis in experimental periodontitis

Localization of bromodeoxyuridine-incorporating, p63- and p75NGFR- expressing cells in the human gingival epithelium

Effects of Aging on Mouse Tongue Epithelium Focusing on Cell Proliferation Rate and Morphological Aspects

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