The effect of age, sex, and lifestyle factors on micronucleus frequency in peripheral blood lymphocytes of the Bosnian population

Nefic, Hilada; Handzic, Inda

doi:10.1016/j.mrgentox.2013.03.001

Cited by 68 publications

(34 citation statements)

References 59 publications

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“…Our results indicate that higher frequency of chromatid and total structural aberrations are most likely related to cigarette smoking and are in agreement with previously reported results for micronuclei frequencies (22). Other analyses of micronuclei frequencies in Bosnian population also revealed the negative impact of cigarette smoking on genetic material (29,30).…”

Section: Discussionsupporting

confidence: 93%

“…The study was conducted with 30 All participants completed informed consent form and questionnaires. The participants were considered eligible for the study under the following criteria: complete information on gender, age, and smoking status was provided, no exposure to ionizing radiation was reported except for natural background levels, and the participants smoked at least two cigarettes per day for ≥1 year before the sampling.…”

Section: Study Groupmentioning

confidence: 99%

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Chromosome aberrations frequency in peripheral blood lymphocytes in young tobacco smoking and non-smoking people

Haverić

Ibrulj

2016

JHSCI

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Introduction: Cigarette smoking is associated with severe health problems, especially cancers. In addition, cigarette smoking causes different genotoxic effects. Chromosome aberrations are one of well-known intermediate end points in carcinogenesis. The aim of this study was to compare frequencies of chromosome aberrations in peripheral blood lymphocytes between young smokers and non-smokes groups.

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Section: Discussionsupporting

confidence: 93%

Section: Study Groupmentioning

confidence: 99%

Chromosome aberrations frequency in peripheral blood lymphocytes in young tobacco smoking and non-smoking people

Haverić

Ibrulj

2016

JHSCI

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“…The possible explanation for this phenomenon was the increase of MN frequency in female that also already found in our previous study [14]. Some studies suspect that the higher of MN frequency in female correlated with greater tendency of the inactive X-chromosome to be lost as an MN relative to other chromosomes [23,24]. …”

Section: Resultssupporting

confidence: 74%

Evaluation of Peripheral Blood Lymphocytes Proliferation in Botteng Village, Mamuju Inhabitants Using Binucleate Index

Ramadhani

Sardini

Masnelli

et al. 2017

J. Ilmiah Aplikasi Isotop dan Radiasi

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Botteng Village in Mamuju, West Sulawesi was known for the high natural background radiation exposure. Botteng Village inhabitants exposed to high natural radiation in their daily life. Radiation exposure can inhibit the mitosis mechanism at various phases. Our previous study revealed that mitotic and nuclear division indexes in Botteng Village inhabitants were lower compared to control samples. To validate our previous study results here we evaluate the binucleate index in peripheral blood lymphocytes of Botteng Village inhabitants. Blood samples were collected from thirteen healthy adult subjects in Botteng Village and thirteen healthy adult subjects in normal background radiation area. Binucleate index was calculated as the proportion of binucleated cell (BNC) in 500 cells for each sample. The study result showed that the BI in Botteng Village was similar compared to control group (23.58 ± 9.60 vs 23.47 ± 6.24). It is possible that the small sample numbers used in this study were not adequate to represent the BI value in Botteng Village inhabitants. This study also showed that there was insignificant difference of BI in respect to gender and age for all samples. Further study using larger sample number should be conducted to verify whether the BI can be used as a marker of lymphocytes proliferation.Keywords : Binucleated cells, Binucleate index, Botteng, Cell proliferation, Natural radiation ABSTRAK Desa Botteng di Mamuju, Sulawesi Barat diketahui memiliki paparan radiasi latar dari alam yang cukup tinggi. Penduduk desa Botteng menerima paparan radiasi alam tinggi dalam kehidupan sehariharinya. Paparan radiasi dapat menghambat proses mitosis dalam berbagai tahapan. Studi yang telah dilakukan menunjukkan bahwa indeks mitosis dan indeks pembelahan inti pada penduduk desa Botteng lebih rendah dibandingkan dengan sampel kontrol. Untuk memvalidasi hasil studi tersebut dilakukan evaluasi terhadap indeks binukleus di limfosit darah tepi penduduk desa Botteng. Sampel darah diperoleh dari tiga belas penduduk dewasa yang sehat di desa Botteng Desa dan daerah dengan radiasi latar normal. Indeks binukleus dihitung sebagai proporsi sel binukleus dalam 500 total sel dari setiap sampel. Hasil studi memperlihatkan bahwa nilai indeks binukleus pada penduduk desa Botteng hampir sama bila dibandingkan dengan kelompok kontrol (23,58 ± 9,60 dan 23,47 ± 6,24). Terdapat kemungkinan bahwa jumlah sampel yang sedikit dalam penelitian yang dilakukan tidak dapat merepresentasikan nilai indeks binukleus di penduduk desa Botteng. Hasil studi memperlihatkan bahwa tidak terdapat perbedaan yang signifikan pada nilai indeks binukleus terkait dengan jenis kelamin dan umur pada semua sampel. Studi lebih lanjut dengan menggunakan jumlah sampel yang lebih besar harus dilakukan untuk memastikan apakah nilai indeks binukleus dapat digunakan sebagai penanda proses pembelahan sel limfosit.

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“…This may have affected the results of studies related to other variables. 28,29 According to Bonassi et al, 27 the effects of gender on the frequency of MN in exfoliated cells are not significant, unlike those observed with lymphocytes (where women tend to exhibit higher frequencies). In the present study, gender did not affect the frequency of MN, although karyolysis and karyorrhexis were more common among men aged ≥60 years, which may be associated with the lower HLI scores exhibited by this group.…”

Section: Discussionmentioning

confidence: 97%

Effects of age on the frequency of micronuclei and degenerative nuclear abnormalities

Ferraz

Neto

Cerqueira

et al. 2016

Rev. bras. geriatr. gerontol.

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The effects of aging, gender and lifestyle factors on inducing chromosomal damage (micronuclei) and nuclear degenerative changes were assessed using the micronucleus test on exfoliated cells of the oral mucosa. The sample included 80 healthy subjects divided into four groups according to age and gender: men and women aged 19-29 years (M19, W19) and men and women aged over sixty years (M60, W60). An interview questionnaire was used to characterize the sample and to determine an index reflecting lifestyle (HLI). The frequency of micronuclei and nuclear degenerative changes was significantly higher among the elderly ( p<0.001) and did not differ by gender among young people ( p>0.05). The occurrence of micronuclei was similar among elderly men and women ( p>0.10), but karyorrhexis and karyolysis were more frequent among men ( p<0.005 and p<0.025, respectively), who also had a lower HLI than the other groups ( p<0.0004). The results of the study indicate that age is the main factor associated with the induction of genetic material damage.

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The effect of age, sex, and lifestyle factors on micronucleus frequency in peripheral blood lymphocytes of the Bosnian population

Cited by 68 publications

References 59 publications

Chromosome aberrations frequency in peripheral blood lymphocytes in young tobacco smoking and non-smoking people

Chromosome aberrations frequency in peripheral blood lymphocytes in young tobacco smoking and non-smoking people

Evaluation of Peripheral Blood Lymphocytes Proliferation in Botteng Village, Mamuju Inhabitants Using Binucleate Index

Effects of age on the frequency of micronuclei and degenerative nuclear abnormalities

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