The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats

Abstract

The aim was to study the influence of albumin supplementation on the changes of the kidney function and structure in cirrhotic rats induced by common bile duct ligation (BDL). Twenty-four male albino rats weighing 200-250 g were divided into Group I: 6 rats underwent laparotomy alone, and the bile duct was only dissected from the surrounding tissue; Group II: 6 rats underwent a sham operation and received 2% albumin in their drinking water; Group III: 6 rats were subjected to bile duct ligation only; and Group IV: 6 rats were subjected to bile duct ligation and received a daily albumin 2% in drinking water. All rats were sacrificed after 4 weeks. We measured the liver and kidney functions and oxidative stress markers in the renal tissue and conducted a histological evaluation of the liver and kidney. The liver enzymes were decreased, but there was no significant difference in the bilirubin levels in group IV compared to group III. There was a significant elevation of serum creatinine in group III compared to group II, and serum creatinine was attenuated in group IV. The renal tissue catalase activity and reduced glutathione, as well as the nitric oxide levels, were significantly increased in group IV and were elevated in group III. Histologically, the livers of group IV showed degeneration and inflammatory cell infiltration with regeneration areas in which normal hepatocytes appeared. The kidneys of group IV showed recovery as well as areas of inflammatory cell infiltration. Some tubules appeared with normal epithelial lining. In conclusion, the results suggest that albumin partially improves the renal functions and structures after their disturbances as a result of BDL.

Serum Albumin Levels of Oral Candidiasis Immunosuppressed Rats Treated With Hyperbaric Oxygen

“…16 Hepatocytes are also being the major part in albumin synthesis, the integrity of these cells will effect on albumin production. 17 This was similar to a previous study that showed a decreased in the amount of albumin caused by a reduced in production by hepatocytes. 19 The impact of hyperbaric oxygen therapy on serum albumin has never been explained in prior studies.…”

“…The decrease in serum albumin levels was likely because of enhanced leakage in the outside vascular system. 17 It also indicates a disruption in liver hepatocytes function, as hepatocytes are an important part of the liver that is responsible for metabolizing drugs. 4 Continuous use of immunosuppressant drugs could resulting in hepatocytes to work harder, which then caused injury and reduced its function.…”

“…15,19 Albumin also known can decrease malondialdehyde levels, increased catalase activity, and glutathione levels significantly, therefore diminished reactive free radicals lead to oxidative damage, protected the tissues from highly reactive hydroxyl radicals, and impaired lipid peroxidation. 17,19 Increased oxygen level derived from oxygen-based therapy will be accompanied by increased production of its product, reactive oxygen and nitrogen species, which has a role in the pathogenesis of the disease. 11,19 However, oxygen therapy given at pressure 2,4 ATA will produce reactive oxygen species as a signaling molecule, in relevant levels for inducing antioxidants activity which can then accelerate the healing process of injured tissues, also will give benefits to regeneration of hepatocyte cells.…”