The effect of alginate oligosaccharides on the mechanical properties of Gram-negative biofilms

Powell, Lydia C.; Sowedan, Ahmed; Khan, Saira; Wright, Chris J.; Hawkins, Karl; Onsøyen, E.; Myrvold, Rolf; Hill, Katja E.; Thomas, David W.

doi:10.1080/08927014.2013.777954

Cited by 79 publications

(64 citation statements)

References 41 publications

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“…The viscosity of biofilm samples was measured using a Physica MCR 301 rheometer (Anton Paar). Biofilms of WT and CP2-1-S1 were prepared by dropping 100 l of overnight cultures (OD 600 , ϳ1.5) onto 0.45-m filter membranes (diameter, 87 mm; Schleicher & Schuell, Dassel, Germany), placing them on MM1 agar, and incubating them at 30°C for 24 h. The membranes with biofilms were then transferred to the rheometer, and the viscosity of each biofilm was determined as previously described (30,31).…”

Section: Methodsmentioning

confidence: 99%

Disruption of Putrescine Biosynthesis in Shewanella oneidensis Enhances Biofilm Cohesiveness and Performance in Cr(VI) Immobilization

Ding

Peng

et al. 2014

Appl Environ Microbiol

122

159

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Although biofilm-based bioprocesses have been increasingly used in various applications, the long-term robust and efficient biofilm performance remains one of the main bottlenecks. In this study, we demonstrated that biofilm cohesiveness and performance of Shewanella oneidensis can be enhanced through disrupting putrescine biosynthesis. Through random transposon mutagenesis library screening, one hyperadherent mutant strain, CP2-1-S1, exhibiting an enhanced capability in biofilm formation, was obtained. Comparative analysis of the performance of biofilms formed by S. oneidensis MR-1 wild type (WT) and CP2-1-S1 in removing dichromate (Cr 2 O 7 2؊ ), i.e., Cr(VI), from the aqueous phase showed that, compared with the WT biofilms, CP2-1-S1 biofilms displayed a substantially lower rate of cell detachment upon exposure to Cr(VI), suggesting a higher cohesiveness of the mutant biofilms. In addition, the amount of Cr(III) immobilized by CP2-1-S1 biofilms was much larger, indicating an enhanced performance in Cr(VI) bioremediation. We further showed that speF, a putrescine biosynthesis gene, was disrupted in CP2-1-S1 and that the biofilm phenotypes could be restored by both genetic and chemical complementations. Our results also demonstrated an important role of putrescine in mediating matrix disassembly in S. oneidensis biofilms.

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Section: Methodsmentioning

confidence: 99%

Disruption of Putrescine Biosynthesis in Shewanella oneidensis Enhances Biofilm Cohesiveness and Performance in Cr(VI) Immobilization

Ding

Peng

et al. 2014

Appl Environ Microbiol

122

159

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“…OligoG CF-5/20 is currently in phase 2b clinical trials in CF patients. Previous in vitro studies have shown the ability of OligoG CF-5/20 to perturb biofilm formation, thereby reducing tolerance to antibiotic treatment (9)(10)(11)(12).…”

mentioning

confidence: 99%

OligoG CF-5/20 Disruption of Mucoid Pseudomonas aeruginosa Biofilm in a Murine Lung Infection Model

Wang

Song

Ciofu

et al. 2016

Antimicrob Agents Chemother

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Biofilm growth is a universal survival strategy for bacteria, providing an effective and resilient approach for survival in an otherwise hostile environment. In the context of an infection, a biofilm provides resistance and tolerance to host immune defenses and antibiotics, allowing the biofilm population to survive and thrive under conditions that would destroy their planktonic counterparts. Therefore, the disruption of the biofilm is a key step in eradicating persistent bacterial infections, as seen in many types of chronic disease. In these studies, we used both in vitro minimum biofilm eradication concentration (MBEC) assays and an in vivo model of chronic biofilm infection to demonstrate the biofilm-disrupting effects of an alginate oligomer, OligoG CF-5/20. Biofilm infections were established in mice by tracheal instillation of a mucoid clinical isolate of Pseudomonas aeruginosa embedded in alginate polymer beads. The disruption of the biofilm by OligoG CF-5/20 was observed in a dose-dependent manner over 24 h, with up to a 2.5-log reduction in CFU in the infected mouse lungs. Furthermore, in vitro assays showed that 5% OligoG CF-5/20 significantly reduced the MBEC for colistin from 512 g/ml to 4 g/ml after 8 h. These findings support the potential for OligoG CF-5/20 as a biofilm disruption agent which may have clinical value in reducing the microbial burden in chronic biofilm infections. It has been suggested that biofilms are present in more than 65% of all bacterial infections (1). Biofilm-associated bacteria show an innate resistance to antibiotics, disinfectants, and host defense mechanisms (2, 3) and are thought to contribute to survival in chronic lung infections. Several in vitro studies have demonstrated that bacteria growing in a biofilm can become 10 to 1,000 times more resistant to the effects of antimicrobial agents than planktonic-growing bacteria of the same strain (4, 5). Patients with cystic fibrosis (CF) are highly susceptible to lung infections. As the disease progresses their lungs become chronically infected by P. aeruginosa as a biofilm infection (6), contributing to the destructive cycle of inflammation and fibrosis (7). Clinically, the presence of mucoid variants is associated with poor prognosis, deterioration of lung function, and increased tissue damage (2, 8). Therefore, the disruption of the biofilm is a key step in eradicating persistent bacterial infections, as is seen in many types of chronic disease.The novel alginate oligomer OligoG CF-5/20 is a low-molecular-weight oligosaccharide enriched from sodium alginate polysaccharides. It is an oligomer composed of ␣-L-guluronic acid (Ͼ85%) and ␤-D-mannuronic acid (Ͻ15%) (Fig. 1). OligoG CF-5/20 is currently in phase 2b clinical trials in CF patients. Previous in vitro studies have shown the ability of OligoG CF-5/20 to perturb biofilm formation, thereby reducing tolerance to antibiotic treatment (9-12).High-molecular-weight polymeric alginate is one of the components produced by P. aeruginosa in the formation of biofilms in the a...

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“…Flos lonicerae, Wuwei xiaodu pulvis, Shuanghuanglian, Chinese gallnut extract and ginseng monomer [40][41][42] have the effect of inhibiting or destroying the biofilm. The bacteriophage AB7-IBB1, AB7-IBB2 and OligoG [27,[43][44][45] can promote dissociation and release of biofilm. Nanoparticles break the existing mechanism of resistance, including the reduction of drug absorption and the inhibition of the formation of Acinetobacter baumannii biofilm.…”

Section: Destruction Of the Formed Biofilm Of Acinetobacter Baumanniimentioning

confidence: 99%

Acinetobacter baumannii biofilm resistance mechanisms and prevention and control of progress

Wang¹,

Wang²

2016

DCC

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At present, the multiple drug resistance of Acinetobacter baumannii outbreaks worldwide and has intensified the trend, especially in the intensive care unit and burn ward. Generic drug resistant Acinetobacter baumannii is known as the 21 st century gram-negative bacterium "MRSA", "Superbugs". In recent years, researches have shown that this is associated with pathogenic bacteria to form biofilms. In this paper, the status of Acinetobacter baumannii infection, biofilm formation, resistance mechanism and prevention in recent years were summarized.

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The effect of alginate oligosaccharides on the mechanical properties of Gram-negative biofilms

Cited by 79 publications

References 41 publications

Disruption of Putrescine Biosynthesis in Shewanella oneidensis Enhances Biofilm Cohesiveness and Performance in Cr(VI) Immobilization

Disruption of Putrescine Biosynthesis in Shewanella oneidensis Enhances Biofilm Cohesiveness and Performance in Cr(VI) Immobilization

OligoG CF-5/20 Disruption of Mucoid Pseudomonas aeruginosa Biofilm in a Murine Lung Infection Model

Acinetobacter baumannii biofilm resistance mechanisms and prevention and control of progress

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