The Effect of Alkaline Earth Cations on the Release of Histamine From Rat Peritoneal Mast Cells Treated With Compound 48/80 and Peptide 401

Abstract

1 Extracellular calcium ions have a dual effect on the release of histamine from rat peritoneal mast cells treated with compound 48/80 and peptide 401. The release is either potentiated or inhibited according to the relative concentrations of ion and inducer. 2 Strontium similarly potentiates the release produced by optimal concentrations of inducer but higher concentrations are required than in the case of calcium. Strontium is markedly less inhibitory than calcium. 3 Mast cells may be depleted of intracellular calcium by incubation for short periods with the chelating agent, ethylenediamine tetraacetic acid (EDTA). They thereby become unresponsive to compound 48/80 and peptide 401 unless calcium is reintroduced into the incubation medium. Strontium and barium, but not magnesium, will substitute for calcium in this system. Barium additionally produces a marked release of histamine even in the absence of inducer. Pretreatment with the ionophore A23187 similarly inhibits the subsequent response to peptide 401 in divalent cation-free medium. This inhibition is reversed on the reintroduction of calcium. 4 Compound 48/80 and peptide 401 release histamine from mast cells incubated in isotonic sucrose in the complete absence of added metal ions. However, the corrected release under these conditions is potentiated by both mono and divalent cations. 5 On the basis of these results, the possible mechanism of action of the basic releasing agents and their usefulness as models for studying histamine secretion is discussed.

Inhibition of Na+ ‐K+ pump activity by divalent cations in intact peritoneal mast cells of the rat

“…Replacement of sodium with an equimolar concentration of lithium almost abolished the uptake and there was a tendency to an increased release of histamine as previously reported (Cochrane & Douglas, 1976;Atkinson et al, 1979). In presence of sodium the non-stimulated secretion was 5.1% + 1.9%, and in presence of lithium it was 8.2% + 3.8% (mean values and s.e.mean from five experiments, P < 0.1).…”

A comparison of histamine secretion from peritoneal mast cells of the rat and hamster

“…Mixed peritoneal cells were recovered by direct lavage (Atkinson et al, 1979) with modified Tyrode solution of the following composition (mM): NaCl 137, glucose 5.6, KCl 2.7, NaH2PO4 0.4, CaCl2 1 and N-2-hydroxyethyl piperazine-N'-2 ethane sulphonic acid (HEPES) 10. The pH of the solution was adjusted to 7.2 before use.…”

“…Individual supernatants were treated similarly. Histamine was then determined spectrofluorimetrically (Atkinson et al, 1979) using a commercial automated apparatus (Technicon Autoanalyzer II). Histamine release was expressed as a percentage of the total cellular content of the amine and was calculated as the ratio (histamine in supernatant)/(histamine in supernatant+ residual histamine in cells) (x 100).…”

Type 1 hypersensitivity reactions in reconstructed tissues using syngeneic cell types

“…Peritoneal cells were prepared by a method adapted from Atkinson et al (1979). Animals were killed by exposure to CO2, (100%) and Krebs Henseleit solution (KHS), 10 ml, was injected into the peritoneal cavity.…”