The effect of allogenic human Wharton's jelly stem cells seeded onto acellular dermal matrix in healing of rat burn wounds

Abstract

Background Various methods were introduced to overcome the autograft shortage in burn wound care, including cell transplantation and tissue engineering. Aims To evaluate the healing effect of allogenic human Wharton's jelly stem cells (hWJSCs) seeded onto acellular dermal matrix (ADM) in rat burn injuries. Patients and Methods Human Wharton's jelly stem cells provided from umbilical cord tissue were characterized before transplantation, and the growth kinetic was determined. Skin samples from cosmetic surgeries were used for preparation of ADM. Forty male Sprague Dawley rats were randomly divided into 4 equal groups. Third‐degree burn was induced for all animals by exposing to hot water using a 2 cm ring for 10 seconds. Group 1 was burned rats that did not receive any treatment. After burn injury, the second group received silver sulfadiazine (SSD), the third group was treated just by using ADM, and the fourth group received 2 × 106 hWJSCs seeded onto ADM. The animals were euthanized for histologic evaluation after 7, 14, and 21 days. Results Human Wharton's jelly stem cells were characterized to be spindle shape and positive for osteogenic and adipogenic induction and for mesenchymal markers but lacked hematopoietic markers. Population doubling time (PDT) was 40.1 hours with an increasing growth trend until day 6th. Macro‐ and microscopically, the healing was mild in ADM group and moderate in ADM + hWJSCs group after 21 days. Conclusion Allogenic hWJSCs seeded onto ADM improved the healing process in burn wounds denoting to their therapeutic and anti‐inflammatory effects in burn wounds that can be added to the literature.

The Effect of Age and Type of Media on Growth Kinetics of Human Amniotic Fluid Stem Cells

“…23 hAFSCs isolated in our study from different age groups of pregnant mothers were positive for CD73, CD90, and CD105 as mesenchymal markers and negative for CD34 and CD45 as hematopoietic markers, confirming the abovementioned studies. 14,15,[20][21][22][23][24] hAFSCs have been shown to have self-renewal ability and a high growth rate, making them a good candidate for cell transplantation. 25 Varghese et al, in a systematic review on 41 articles regarding factors affecting adipose tissue stem cell viability and function, demonstrated a decreased proliferation and differentiation potential of adipose tissuederived stem cells (AdSCs) with an increase in age.…”

Current Advanced Therapies Based on Human Mesenchymal Stem Cells for Skin Diseases

“…At preclinical level, five studies [one CT (Pourfath et al, 2018) and four TE strategies (Steffens et al, 2017;Kaita et al, 2019;Mahmood et al, 2019;Nazempour et al, 2020)] have evaluated the use of hMSCs for burn injuries in the last 5 years ( Table 3).…”

“…In this case, each of the four studies reported, used a different hMSCs population (Table 3): deciduous teeth (hDT-MSCs) (Steffens et al, 2017), hUCB-MSCs (Mahmood et al, 2019), hAT-MSCs (Kaita et al, 2019) and hWJ-MSCs (Nazempour et al, 2020). The average follow-up of these studies was 15.5 ± 5.9 days and third-degree burns in mice and rats was the preferred model, although the type of TESS evaluated varied.…”

“…Finally, Nazempour et al (2020) evaluated the use of 2 × 10 6 hWJ-MSCs seeded onto acellular dermal matrix scaffold and compared the results with a conventional treatment (silver sulfadiazine ointment). Wound size decreased in a better way in group where hWJ-MSCs were used (87.6%).…”

Therapeutic Effect of Mesenchymal Stem Cells in Ulcerative Colitis: A Review on Achievements and Challenges

“…They express cell surface markers such as CD44, CD73, CD90, and CD105, and lack the expression of hematopoietic markers such as CD34 and CD45 [21]. They have been isolated from various sources such as bone marrow (BM) [22], adipose tissue [23], and umbilical cord Wharton's jelly [24]. MSCs have therapeutic effects in various inflammatory diseases due to its hypoimmunogenic and immunoregulatory properties and can be the home to the site of injury, limit inflammation through cytokine release, stimulate healing through growth factor expression, alter host immune responses through secretion of immune-modulatory proteins, and secrete anti-apoptotic factors [25].…”