The Effect of Allopurinol Pretreatment Before Detorting Testicular Torsion

Akgür, Feza M.; Kılınç, Kamer; Aktuğ, Tanju; Olguner, Mustafa

doi:10.1016/s0022-5347(17)35351-x

Cited by 80 publications

(56 citation statements)

References 6 publications

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“…Akgür, Lysiak, Bozlu, Abasıyanık and Ozkan et al showed that allopurinol, catalase and catalase plus superoxide dismutase and poly (ADP-ribose) polymerase inhibitors, melatonin and zinc aspartate treatments decreased the lipid peroxidation product (MDA). 9,15,18,19,37 We found that MDA level was significantly decreased by trapidil in testicular torsion and detorsion in our study also.…”

Section: Discussionsupporting

confidence: 71%

Protective effects of trapidil in ischemia–reperfusion injury due to testicular torsion and detorsion: An experimental study

Somuncu¹,

Çakmak²,

Erdoğan³

et al. 2006

Int J of Urology

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Objective: We aimed to detect the preventive effects of trapidil in ischemia-reperfusion (IR) injury due to testicular torsion and detorsion. Methods: Forty prepubertal albino rats were used. In the IR group, torsion was created by rotating the left testis over 2 h, and detorsion was done by untwisting the testis. Bilateral orchiectomies were performed after 4 h. In study group, 2-h torsion was performed and trapidil was administered as a single dose 1 h before detorsion. Bilateral orchiectomies were performed after 4 h. In the sham group, a sham operation was done. In the sham plus trapidil group, a sham operation was done and trapidil was administered as a single dose. Testicular tissue malondialdehyde (MDA), nitric oxide (NO) and total sulfhydryl (T-SH) levels were determined for each group. The grades of interstitial injury were determined in histopathologic examination. Results: The NO and MDA levels in the IR group were significantly higher than the study, sham and sham plus trapidil groups in the left testis (P < 0.05, P < 0.001 and P < 0.001, respectively). A statistical difference was not found among study, sham and sham plus trapidil groups in the left testis in NO and MDA levels (P > 0.05). The T-SH level in the study group was significantly higher than in the IR, sham and sham plus trapidil groups in left testis P < 0.05). In the IR group (left testis), grade 1 interstitial injury was 30% (3/10), grade 2 injury was 60% (6/10) and grade 3 injury was 10% (1/10). In the study group (left testis), grade 1 interstitial injury was 30% (3/10) and there was no injury in 70% (7/10). Conclusion: Trapidil decreased free oxygen radical formation in testicular torsion and detorsion, and attenuated histopathological damage in the ipsilateral twisted testis.

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Section: Discussionsupporting

confidence: 71%

Protective effects of trapidil in ischemia–reperfusion injury due to testicular torsion and detorsion: An experimental study

Somuncu¹,

Çakmak²,

Erdoğan³

et al. 2006

Int J of Urology

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“…9 The significant increase of MDA levels at the end of the ischemia and during the reperfusion in rats submitted to the torsion of the spermatic cord show that 3-h ischemia promotes severe cell membrane peroxidation (Figure 1). On the other hand, the additional increase of MDA levels 1h and 3 h after detorsion of the spermatic cord reassures that additional injuries occur during reperfusion as has been reported previously by Akgur et al 10 Glutathione (GSH) is a tripeptide, L-g-glutamyl-L-cysteinyl-glycine, mol. wt.…”

Section: Discussionsupporting

confidence: 68%

Oxidative stress induced by torsion of the spermatic cord in young rats

Guimarães¹,

Aragão²,

Santos³

et al. 2007

Acta Cir. Bras.

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Purpose:To evaluate the effects of the oxidative stress in an experimental model of torsion/detorsion of the spermatic cord and the legitimacy of this model for oxidative stress studies. Methods: Forty-eight male Wistar rats were randomized in two groups (n=24): G-1 (Sham) and G-2 (Ischemia/Reperfusion). All rats received intraperitoneal saline injections (2.0 ml), at 21, 9, and 1 h before right spermatic cord torsion or first sham operation. Detorsion or second sham operation was carried out 3 h later followed by testis and blood samples collection (T-0). Additional samples were collected at 1-3-6 h time-points for assessment of testis malonaldehyde, glutathione, and plasma total antioxidant power (TAP). Results: Spermatic cord torsion/detorsion induced a significant increase in testicular malonaldehyde contents and a significant decrease in glutathione concentrations in ischemic rats compared with sham animals. Additional increase in malonaldehyde levels occurred during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study. Conclusion: Torsion/detorsion of the spermatic cord for 3 h induces significant lipid peroxidation and reduction in glutathione content of the testis and is, therefore, a valid model for studying the oxidative stress effects of the ischemia/ reperfusion injury in young rat testis. Key words: Antioxidants. Testis. Oxidative stress. Malonaldehyde. Glutathione. Rats. RESUMOObjetivo: Investigar os efeitos do estresse oxidativo utilizando um modelo experimental de torção/destorção do cordão espermático e a aplicabilidade do modelo para estudo do estresse oxidativo. Métodos: Foram utilizados 48 ratos distribuídos aleatoriamente em dois grupos (n=24): G-1 (Simulado) e G-2 (Isquemia/Reperfusão). Todos os animais receberam injeções intraperitoniais de solução salina (2,0 ml) 21-9-1 horas antes da torção ou primeira operação simulada. A destorção/ segunda operação simulada (T-0) foi realizada após 3 horas, com coleta das amostras (testículo e sangue arterial) nesta ocasião e após 1-3-6 horas para determinação das concentrações de malonaldeído, glutationa e capacidade antioxidante total do plasma (CAT). Resultados: a torção do cordão espermático induziu aumento significante das concentrações de malonaldeído e redução simultânea das concentrações de glutationa nos ratos do grupo G-2, comparados ao grupo simulado. Ocorreu aumento adicional das concentrações de malonaldeído durante a reperfusão (G-2). A capacidade antioxidante total do plasma foi semelhante nos dois grupos estudados, denotando ausência de efeitos sistêmicos. Conclusão: A torção durante 3 horas seguida de destorção do cordão espermático induz um nível significante de peroxidação lipídica e redução da concentração de glutationa constituindo-se um modelo válido para o estudo dos efeitos do estresse oxidativo decorrente da lesão de isquemia/reperfusão no testículo de ratos jovens.

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“…10 experiment elicited a significant increase in TBARS in torted testis (p<0.01) and further increase was caused by detorsion (reperfusion). Pretreatment with allopurinol before detorsion prevented reperfusion injury.…”

Section: Discussionmentioning

confidence: 95%

“…Different therapeutic strategies have been investigated with the aim of reducing testis ischemia/reperfusion damage [10][11][12][13] . The aim of the present study was to evaluate, in an experimental model of testis I/R the effects of DMSO on tissue and plasma oxidative stress in twisted testis after unilateral torsion and detorsion.…”

Section: Introductionmentioning

confidence: 99%

Dimethylsulfoxide attenuates ischemia-reperfusion injury in rat testis

Guimarães¹,

Kimura²,

Vasconcelos³

2010

Acta Cir. Bras.

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Purpose:To evaluate the protective role of dimethylsulfoxide (DMSO) in a rat model of testis ischemia/reperfusion (I/R). Methods: Twenty-four male Wistar rats were randomized in two equal groups. Control rats (G-1) received saline 2.0 ml intraperitoneally (ip) 21, 9 and 1 h before torsion. Experimental rats (G-2) received ip injections of 3% aqueous solution of DMSO, 0.1ml/10g body weight. Saline was added to complete 2.0ml when necessary. I/R injury was induced in anesthetized rats by torsion of the right testis lasting 3 hours. Testis and blood samples were collected at the end of ischemia (T-0) and 3 hours later (T-3) for assessment of testis malonaldehyde (MDA), reduced glutathione (GSH), and plasma total antioxidant power (TAP). Results: MDA levels decreased significantly in G-2 rats compared with G-1 animals in all time-points. GSH levels increased significantly in T-0 and T-3 time-points in DMSO pretreated rats compared with G-1 rats. GSH levels increased significantly during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study. Conclusion: Pretreatment with DMSO reduces testis lipid peroxidation and oxidative stress caused by torsion/detorsion of the testis. Key words: Antioxidants. Testis. Oxidative Stress. Malondialdehyde. Glutathione. Rats. RESUMO Objetivo:Avaliar o papel protetor do dimetilsulfóxido (DMSO) utilizando um modelo de isquemia/reperfusão (I/R) do testículo do rato. Métodos: Vinte e quatro ratos machos Wistar, randomizados em dois grupos iguais e tratados com solução salina 2,0 ml (grupo Controle-G-1) ou solução de DMSO (3%, 0,1ml/10g peso corporal), acrescida de solução salina até completar 2,0 ml (grupo Experimento-G-2) por via intraperitoneal, as 21, 9 e 1 h antes da torção. .A lesão de I/R foi induzida por torção do testículo direito e mantida por 3 horas, em ratos previamente anestesiados. Amostras (sangue e testículo direito) foram coletadas ao término da isquemia (T-0) e após 3 horas de reperfusão (T-3) para determinação das concentrações de malonaldeído (MDA), glutationa reduzida (GSH) e capacidade antioxidante total do plasma (CAT). Resultados: As concentrações de MDA diminuíram significativamente nos ratos do G-2. comparados ao G-1, nos tempos estudados. As concentrações de GSH aumentaram significativamente nos testículos dos ratos do G-2 comparado aos controles no T-0 e no T-3 e durante a reperfusão no G-2. Os valores obtidos na análise do CAT foram semelhantes. Conclusão: O pré-tratamento com DMSO reduz a peroxidação lipídica e o estresse oxidativo decorrentes da torção/destorção do testículo, no rato.

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The Effect of Allopurinol Pretreatment Before Detorting Testicular Torsion

Cited by 80 publications

References 6 publications

Protective effects of trapidil in ischemia–reperfusion injury due to testicular torsion and detorsion: An experimental study

Protective effects of trapidil in ischemia–reperfusion injury due to testicular torsion and detorsion: An experimental study

Oxidative stress induced by torsion of the spermatic cord in young rats

Dimethylsulfoxide attenuates ischemia-reperfusion injury in rat testis

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