The effect of alpha lipoic acid on hydrogen peroxide-induced lipid peroxidation in rat liver homogenates

Abstract

Alfa lipoik asidin rat karaciğer homojenatlarında hidrojen peroksit ile indüklenmiş lipid peroksidasyonuna etkisi [The effect of alpha lipoic acid on hydrogen peroxide-induced lipid peroxidation in rat liver homogenates] ABSTRACT Objective: It is known that oxidant species play a role in the pathogenesis of certain diseases such as diabetes mellitus, atherosclerosis, cataract and hepatic cirrhosis. Therefore, the use of antioxidant species for therapeutic purposes has risen up in the recent years. The aim of the study was to investigate the different concentrations of alpha lipoic acid on the induced lipid peroxidation and tissue glutathione level in rat liver homogenates. Methods: Hydrogen peroxide (15 mM) has been used for induction of lipid peroxidation at liver homogenates. The experimental setups induced by lipid peroxidation have been divided into four sub-groups. Alpha lipoic acid was added in 0, 2, 4 and 8 mM concentrations into those groups, respectively. The malondialdehyde levels which is the end product of lipid peroxidation and the levels of tissue glutathione have been determined. Results: The level of malondialdehyde in the activation groups has been found to be significantly higher than to the control group. The levels of malondialdehyde in the all alpha lipoic acid groups have been found to be significantly lower than the activation group. The level of glutathione in the activation group has been detected significantly lower when it was compared to the control group. The levels of glutathione in the all alpha lipoic acid groups have been found to be significantly higher from the activation group. When the time-dependent change in the level of glutathione was investigated it was observed that these initially decrease and then started to increase. In the groups of 4 and 8 mM, this level was even over from the starting point. Conclusion: In conclusion, our findings suggest that lipid peroxidation is induced in the experimental setups where hydrogen peroxide are applied. The reason of significantly lower malondialdehyde and higher glutathione levels in alpha lipoic acid group than activation groups may be a result of the antioxidant property of alpha lipoic acid.

Hepatoprotective effect of allicin against acetaminophen‐induced liver injury: Role of inflammasome pathway, apoptosis, and liver regeneration

“…[36] It has been demonstrated that excess lipid peroxidation is related to the tissue damage in APAP-stimulated hepatotoxicity. [37] Therefore, we measured the level of MDA in the liver tissues of the APAPtreated group. Our results showed that APAP significantly increased the MDA level, and this elevation in the MDA level was significantly inhibited by allicin treatment.…”

Effects of alpha lipoic acid on metabolic syndrome: A comprehensive review

“…In several literature data, pancreatic β‐cells exposed to some particular compounds such as oleic acid and hydrogen peroxide led to an increase in the intracellular oxidative markers such as MDA and ROS formation. Also, GSH content and ATP generation were decreased (B. W. Lee et al, 2009; W. Shen et al, 2008; Yapar & Eskiocak, 2014). Hence, mitochondrial impairment and ROS formation might result in pancreatic toxicity and inhibition of insulin secretion (T. Huang & Huang, 2006; Targonsky et al, 2006).…”

“…Also, GSH content and ATP generation were decreased (B. W. Lee et al, 2009;W. Shen et al, 2008;Yapar & Eskiocak, 2014).…”

The effect of sulforaphane on oxidative stress and inflammation in rats with toxic hepatitis induced by acetaminophene

“…As a result of such deterioration in the membrane structure, some changes occur in membrane functions such as ion transport and enzyme activity. Free radicals and lipid peroxides are admitted to play a role in the development of various diseases such as diabetes, atherosclerosis, cancer and liver cirrhosis, while lipid peroxidation is suggested to be closely related to the tissue damage from acetaminophen-induced hepatotoxicity (36,37). In addition, the superoxide radical and singlet oxygen are reported to induce liver damage through lipid peroxidation (38).…”