The effect of aminoacetonitrile on calcium metabolism and bone in the rat.

Kemm, John

doi:10.1113/jphysiol.1976.sp011545

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…No significant rise in serum calcium level in chronically parathyroidectomized rat has been shown if they were fed the low calcium diet as likely as0.2% calcium. In this situation, possible role of1,25-dihydroxycholecalciferol has not been proved by Kemm (1976).…”

Section: Discussionmentioning

confidence: 95%

“…Prostaglandin E2which produces an elevation of the serum calcium in intact rats when infused continuously (Franklin and Tashjian, 1975), the osteoclast-activating factor of lymphoid cells in elevating serum calcium level via bone resorption (Horton et al, 1972) or any unknown factors can be involved to control the level of serum calcium under the conditions where parathyroid hormone is deprived, and/or where thyroid hormone and calcitonin are deprived. The rise in serum calcium has been noted in chronically parathyroidectomized rats adapted to the higher calcium diet (Kemm, 1976). No significant rise in serum calcium level in chronically parathyroidectomized rat has been shown if they were fed the low calcium diet as likely as0.2% calcium.…”

Section: Discussionmentioning

confidence: 98%

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Sex difference in adaptation of serum calcium level to the gradual deprivation of parathyroid hormone and to the following thyroidectomy.

Fujii

Morita

1980

Endocrinol Japon

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The time course of adaptation of the serum calcium level to the gradual deprivation of parathyroid hormone was studied in adult male and female rats. In addition, the role of thyrocalcitonin and thyroid hormones in adaptive changes in serum calcium was assessed in the surgically parathyroidectomized animals. Simultaneous bilateral parathyroidectomy, thyroparathyroidectomy or the second unilateral parathyroidectomy performed72hr after the first unilateral parathyroidectomy produced a prompt fall in serum calcium level leading to the lowest value at10hr and the significantly lower calcium level retained for one week-observation. Unilateral parathyroidectomy resulted in a transient fall in the serum calcium level and to a rapid return to the subnormal range in24hr in both sexes. When the second unilateral parathyroidectomy was carried out1or6weeks after the first unilateral parathyroidectomy, the serum calcium in female rats showed a slight decrease until10hr and returned to the normal level in one week, but in male rats no further decline or a gradual decrease was seen. Bilateral thyroidectomy performed10weeks after the second unilateral parathyroidectomy resulted in a gradual increase in the serum calcium above the normal range in female rats. In male rats, the serum calcium level tended to drop until10hr and returned to the initial level or to the normal range. Bilateral thyroidectomy performed one week after simultaneous bilateral parathyroidectomy led to a further decline in serum calcium in both male and female rats. The results indicate that adaptation of the serum calcium level toward the normal range occurs in the absence of parathyroid hormone in adult male and female rats if the parathyroid hormone is gradually deprived. A greater ability of female rats to restore the calcium level, especially seen when thyroidectomized after adaptation to the lack of parathyroid hormone, suggests that estrogens play a significant role in the homeostatic control of serum calcium in chronic disorders in calcium regulation.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 98%

Sex difference in adaptation of serum calcium level to the gradual deprivation of parathyroid hormone and to the following thyroidectomy.

Fujii

Morita

1980

Endocrinol Japon

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“…9,26). This effect may be caused by the non-specific toxic action of the drugs and/or by the reduction of food and water intakes (27,28). After cessation of the daily administration of AAN, the body weight of the animals began to be restored progressive!y (Tab!e 4).…”

Section: Discussionmentioning

confidence: 99%

Effects of lathyrogens on the mechanical strength of the periodontal ligament in the rat mandibular first molar

Ohshima

Nakamura

Chiba

1989

J of Periodontal Research

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Effects of lathyrogens such as aminoacetonitrile (AAN), beta-aminopropionitrile (BAPN) and cysteamine--known inhibitors of cross-linking of collagen--on the mechanical strength of the periodontal ligament of the rat mandibular first molar were examined by measuring the ultimate load required to extract the tooth from its socket in the dissected jaw. Single injections of AAN (40 approximately 100 mg/100 g body weight) or of BAPN (100 mg/100 g body weight) caused significant decreases of the mechanical strength 24 h after administration of the drugs but that of cysteamine (30 mg/100 g body weight) did not. Significant correlations between the dose of AAN or of BAPN and the mechanical strength were found following daily administrations of the drugs for 5 days. The relative potency of AAN to BAPN was estimated to be 4.5 by a slope ratio assay. The rapid appearance and disappearance of the effect of lathyrogens on the mechanical strength of the periodontal ligament of the rat mandibular first molar provide further evidence that the turnover of the collagen in the tissue is fast. The half-time of collagen synthesis was estimated to be approximately 3 d.

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“…1 Lathyrogens act on the enzyme lysyl oxidase and specifically inhibit the formation of cross-links between collagen molecules. 3 A lathyritic agent, b-aminoproprionitrile (b-APN), was isolated from the sweet pea, L. odouratus, by Selye in 1957 and is considered an appropriate agent for studying connective tissue metabolism. 3 A lathyritic agent, b-aminoproprionitrile (b-APN), was isolated from the sweet pea, L. odouratus, by Selye in 1957 and is considered an appropriate agent for studying connective tissue metabolism.…”

Section: Introductionmentioning

confidence: 99%

“…2 Lathyritic agents cause weakness and deformity in connective tissues, preventing normal maturation of collagen by inhibiting the formation of cross-links. 3 A lathyritic agent, b-aminoproprionitrile (b-APN), was isolated from the sweet pea, L. odouratus, by Selye in 1957 and is considered an appropriate agent for studying connective tissue metabolism. 4,5 Experimental lathyrism shows affinity for tissues with high collagen content such as cartilage, bone, and fibrous and elastic connective tissue.…”

Section: Introductionmentioning

confidence: 99%

The role of ascorbic acid on collagen structure and levels of serum interleukin-6 and tumour necrosis factor-alpha in experimental lathyrism

et al. 2004

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Lathyrism is characterized by defective collagen synthesis due to inhibition of lysyl oxidase, an enzyme essential for interfibrillar cross-linking. The lathyritic agent beta-aminoproprionitrile (beta-APN) is considered an appropriate agent for studying connective tissue metabolism. We investigated the effects of ascorbic acid on collagen structure and serum cytokine levels in experimentally induced lathyrism. Forty Wistar rats weighing 200-300 g were used in the study: three test groups of 10 rats each (groups 2, 3 and 4) and 10 rats used as a control group (group 1). Experimental lathyrism was induced with daily subcutaneous injections of beta-APN in the test groups for 40 days. On the 40th day, skin biopsies were taken from the control group (group 1) and group 2, to evaluate the effect of beta-APN on dermal collagen. After the 40th day, 10 rats received ascorbic acid 100 mg/kg intraperitoneally daily for 15 days (group 3) and 10 rats (group 4) received no medication and served as a control for group 3. On the 55th day, skin biopsies were taken from groups 3 and 4. Serum concentrations of interleukin-6 and tumour necrosis factor-alpha were assessed in each group by enzyme-linked immunosorbent assay. Ultrastructural examination of the skin biopsies in group 1 revealed normal-appearing epidermal and dermal structures. Group 2 showed disorganization of the epidermis and collagen structure, and vacuolization of the endoplasmic reticulum in fibroblasts. In group 3, ultrastructural examination revealed significant improvement in the structure of dermal collagen after administration of ascorbic acid, whereas the changes in group 4 were unremarkable. Ascorbic acid administration significantly decreased the concentrations of serum cytokines in group 3 compared with group 2 (P < 0.001). Ascorbic acid administration significantly improved dermal collagen structure and serum cytokine levels in experimental lathyrism.

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The effect of aminoacetonitrile on calcium metabolism and bone in the rat.

Abstract: 8. In some experiments parathyroidectomy or thyroparathyroidectomy protected the bones from the effect of AAN.

Cited by 7 publications

References 10 publications

Sex difference in adaptation of serum calcium level to the gradual deprivation of parathyroid hormone and to the following thyroidectomy.

Sex difference in adaptation of serum calcium level to the gradual deprivation of parathyroid hormone and to the following thyroidectomy.

Effects of lathyrogens on the mechanical strength of the periodontal ligament in the rat mandibular first molar

The role of ascorbic acid on collagen structure and levels of serum interleukin-6 and tumour necrosis factor-alpha in experimental lathyrism

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