The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

Nazari, Hassan; Shirazi, Abolfazl; Shams‐Esfandabadi, Naser; Afzali, Azita; Ahmadi, Ebrahim

doi:10.1387/ijdb.160010hn

Cited by 16 publications

(10 citation statements)

References 59 publications

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“…To the best of our knowledge, this study is the first report on the use of MSCs in equine NT, but their potential as nuclear donors has been demonstrated before in other mammalian species. As previously reported, higher in vitro preimplantation development was observed in bovine, 12 porcine 38 and goat 14 with MSCs as nuclear donors compared to fibroblasts. In the porcine, embryos reconstructed with adipose tissue MSCs (aMSCs) resulted in higher blastocyst rates compared to peripheral blood MSCs or fibroblast-reconstructed embryos, 11 which reflects the variability among different MSC sources.…”

Section: Discussionsupporting

confidence: 77%

“…Skin fibroblasts are the most common cells used, but cells derived from liver, 2 kidney, 3 granulosa, 4 , 5 and mesenchymal stem cells (MSCs) have also been tested. In the last years, cloning with MSCs has been evaluated in different farm animal species, such as porcine, 6 – 11 bovine, 12 , 13 caprine, 14 ovine, 15 and equine. 16 Although higher blastocyst rates were observed by using MSCs instead of fibroblasts in most reports, only porcine and bovine offspring has been obtained from MSC cloning procedures.…”

Section: Introductionmentioning

confidence: 99%

“… 6 , 7 , 11 , 13 The developmental ability of embryos reconstructed using MSCs has been associated with their epigenetic, imprinting and pluripotency gene expression profile, which were similar to in vitro fertilized embryos. 7 , 12 In the horse, MSC-derived embryos also showed higher blastocyst rates than fibroblast-derived embryos, but no offspring was obtained. 16 However, this was the only report that evaluated equine MSCs’ potential as nuclear donors, and only a single MSC line derived from the umbilical cord was used in this case.…”

Section: Introductionmentioning

confidence: 99%

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Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses

Olivera¹,

Moro²,

Jordan³

et al. 2018

SCCAA

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IntroductionCell plasticity is crucial in cloning to allow an efficient nuclear reprogramming and healthy offspring. Hence, cells with high plasticity, such as multipotent mesenchymal stem cells (MSCs), may be a promising alternative for horse cloning. In this study, we evaluated the use of bone marrow-MSCs (BM-MSCs) as nuclear donors in horse cloning, and we compared the in vitro and in vivo embryo development with respect to fibroblasts.Materials and methodsZona-free nuclear transfer was performed using BM-MSCs (MSC group, n=3432) or adult fibroblasts (AF group, n=4527). Embryos produced by artificial insemination (AI) recovered by uterine flushing and transferred to recipient mares were used as controls (AI group).ResultsBlastocyst development was higher in the MSC group than in the AF group (18.1% vs 10.9%, respectively; p<0.05). However, pregnancy rates and delivery rates were similar in both cloning groups, although they were lower than in the AI group (pregnancy rates: 17.7% [41/232] for MSC, 12.5% [37/297] for AF and 80.7% [71/88] for AI; delivery rates: 56.8% [21/37], 41.5% [17/41] and 90.1% [64/71], respectively). Remarkably, the gestation length of the AF group was significantly longer than the control (361.7±10.9 vs 333.9±8.7 days), in contrast to the MSC group (340.6±8.89 days). Of the total deliveries, 95.2% (20/21) of the MSC-foals were viable, compared to 52.9% (9/17) of the AF-foals (p<0.05). In addition, the AF-foals had more physiological abnormalities at birth than the MSC-foals; 90.5% (19/21) of the MSC-delivered foals were completely normal and healthy, compared to 35.3% (6/17) in the AF group. The abnormalities included flexural or angular limb deformities, umbilical cord enlargement, placental alterations and signs of syndrome of neonatal maladjustment, which were treated in most cases.ConclusionIn summary, we obtained 29 viable cloned foals and found that MSCs are suitable donor cells in horse cloning. Even more, these cells could be more efficiently reprogrammed compared to fibroblasts.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses

Olivera¹,

Moro²,

Jordan³

et al. 2018

SCCAA

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“…Total RNA was reverse transcribed into cDNA in immediately after extraction (less than 2 hr) using M-MLV reverse transcriptase (Sinaclon Bioscience) as described by Nazari, Shirazi, Shams-Esfandabadi, Afzali & Ahmadi, (2016). The reverse transcription was done in a 20 µl volume containing 10 µl (14 µg) of extracted RNA and 1 µl random hexamer.…”

Section: Rna Extraction and Cdna Synthesismentioning

confidence: 99%

Cryopreservation and its effects on motility and gene expression patterns and fertilizing potential of bovine epididymal sperm

Nazari

Ahmadi

Hamid

et al. 2020

Veterinary Medicine & Sci

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Despite encountering new challenges in using epididymal sperm recovered from cauda epididymides, this accessible and, in some species, worthwhile sample makes inevitable the further development of a suitable cryopreservation protocol. In this study, sperm was recovered from the epididymis of 4°C overnight stored slaughtered bulls' testes and the effects of cryopreservation on the bovine epididymal sperm motility (with CASA) and gene expression patterns (with quantitative Real time‐PCR) were evaluated. Moreover the fertilizing potential of cryopreserved epididymal sperm was used in in vitro fertilization (IVF). After freezing and thawing of epididymal sperm, total and slow progressive sperm motility, VCL, VAP, MAD, ALH and BCF were significantly decreased (p < .05), while in the parameters of fast progressive motility, VSL, LIN, WOB and STR there were not any significant variations in the frozen sperm compared to fresh (non‐frozen) counterpart. The assessment of abundance of transcripts encoding motility (TSSK6) and fertility (PRM1 and PRM2)‐related genes in epididymal sperm, showed that these transcripts were affected by freezing especially in slow progressive motility status (p < .01). Furthermore, cleavage and blastocyst rate did not present any significant differences between bovine embryos produced in vitro by fresh or frozen‐thawed epididymal sperm. It can be concluded that epididymal sperm has enough freezability after overnight testes storage, and cryopreservation could not affect the percentage of in vitro produced embryos in spite of the changes of relative abundance of some transcripts and direction progressive motility pattern of sperm.

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“…Although many studies have been published, most have been based on retrospective analyses 78798081. Currently several guidelines recommend administering full-dose anticoagulants if the platelet count exceeds 50 × 10 9 /L 8283.…”

Section: Special Considerations In Patients With Catmentioning

confidence: 99%

Current Management of Cancer-associated Venous Thromboembolism: Focus on Direct Oral Anticoagulants

2019

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Cancer-associated venous thromboembolism (CAT) is a common complication associated with high morbidity and mortality. In accordance with major clinical trials comparing low-molecular-weight heparin (LMWH) with a vitamin K antagonist (VKA), LMWH is currently the standard treatment for CAT, owing to its efficacy for thrombosis recurrence and improved safety profile compared to VKA. Over the past few years, direct oral anticoagulants (DOACs) have emerged as potential alternative therapies to LMWH due to their convenient route of administration and predictable pharmacokinetics, but evidence for their use in CAT is inconclusive, as only a small fraction of the study populations in these trials had CAT. Recently, two large head-to-head trials comparing DOACs to LMWH in CAT patients reported comparable efficacies of DOACs with increased bleeding risk. Occasionally, CAT treatment can be challenging due to the heterogeneity of underlying malignancies and comorbidities. Renal insufficiency and gastrointestinal defects are the main obstacles in anticoagulant selection. Careful choice of treatment candidates and proper anticoagulant strategies are critical for the treatment of CAT; hence, more studies are required to address these challenges.

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The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

Cited by 16 publications

References 59 publications

Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses

Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses

Cryopreservation and its effects on motility and gene expression patterns and fertilizing potential of bovine epididymal sperm

Current Management of Cancer-associated Venous Thromboembolism: Focus on Direct Oral Anticoagulants

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