The effect of amphotericin B on the permeability of lipid bilayers to divalent trace metals

Aggett, Peter; Fenwick, Paul K.; Kirk, Hannah

doi:10.1016/0005-2736(82)90020-7

Cited by 19 publications

(7 citation statements)

References 11 publications

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“…For patch-clamp recording of whole-cell Ca 2+ currents (I Ca ) the perforated-patch mode of the patch-clamp technique was used [26–27], using amphotericin B (1mg/ml) as the permeating agent [28–29]. Tight seals (>1 GΩ) were achieved with the intracellular solution that had the following composition (in mM): 145 Glutamic Acid, 9 NaCl, 1 MgCl 2 and 10 HEPES, pH 7.2~7.3, adjust with CsOH.…”

Section: Methodsmentioning

confidence: 99%

Regulation of Ca2+ signaling by acute hypoxia and acidosis in rat neonatal cardiomyocytes

Fernández‐Morales¹,

Morad

2018

Journal of Molecular and Cellular Cardiology

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Our studies suggest that acute hypoxia suppresses I in rapidly inactivating cell population by a mechanism involving Ca-dependent inactivation, while compromised mitochondrial Ca uptake seems also to contribute to I suppression in slowly inactivating cell population. Proximity of cellular Ca pools to sarcolemmal Ca channels may contribute to the variability of inactivation kinetics of I in the two cell populations, while acidosis suppression of I appears mediated by proton-induced block of the calcium channel.

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Section: Methodsmentioning

confidence: 99%

Regulation of Ca2+ signaling by acute hypoxia and acidosis in rat neonatal cardiomyocytes

Fernández‐Morales¹,

Morad

2018

Journal of Molecular and Cellular Cardiology

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“…To avoid washout of cytoplasm constituents, the perforated-patch mode (36,44) of the patch-clamp technique was used. Amphotericin B was the permeating agent (1,56). Seals were achieved in a standard Tyrode solution composed of (in mM) the following: 137 NaCl, 1 MgCl 2, 2 CaCl2, 5.3 KCl, 10 glucose, and 10 HEPES.…”

Section: Methodsmentioning

confidence: 99%

Electrophysiological and morphological features underlying neurotransmission efficacy at the splanchnic nerve-chromaffin cell synapse of bovine adrenal medulla

Diego

2010

American Journal of Physiology-Cell Physiology

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“…Whole-cell Ca 2+ currents (I Ca ) was recorded using the perforated-patch mode of the patchclamp technique [22,23], induced by amphotericin B (1mg/ml) [24,25]. After waiting approximately 5-10 minutes, series resistance fell below 20 MΩ and tight seals (>1 GΩ) were achieved using an intracellular solution composed (in mM): 145 Glutamic Acid, 9 NaCl, 1 MgCl 2 and 10 HEPES, pH 7.2~7.3, adjust with CsOH.…”

Section: Electrophysiological Recordingsmentioning

confidence: 99%

Regulation of Ca2+ signaling by acute hypoxia and acidosis in cardiomyocytes derived from human induced pluripotent stem cells

Fernández‐Morales¹,

Hua²,

Yao³

et al. 2019

Cell Calcium

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Aims:The effects of acute (100s) hypoxia and/or acidosis on Ca 2+ signaling parameters of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are explored here for the first time.Methods and Results: 1) hiPSC-CMs express two cell populations: rapidly-inactivating I Ca myocytes (τ i <40ms, in 4-5 day cultures) and slowly-inactivating I Ca (τ i ≥ 40ms, in 6-8 day cultures). 2) Hypoxia suppressed I Ca by 10-20% in rapidly-and 40-55% in slowly-inactivating I Ca cells. 3) Isoproterenol enhanced I Ca in hiPSC-CMs, but either enhanced or did not alter the hypoxic suppression. 4) Hypoxia had no differential suppressive effects in the two cell-types when Ba 2+ was the charge carrier through the calcium channels, implicating Ca 2+ -dependent inactivation in O 2 sensing. 5) Acidosis suppressed I Ca by ~35% and ~25% in rapidly and slowly inactivating I Ca cells, respectively. 6) Hypoxia and acidosis suppressive effects on Ca-transients depended on whether global or RyR2-microdomain were measured: with acidosis suppression was ~25% in global and ~37% in RyR2 Ca 2+ -microdomains in either cell type, whereas with hypoxia suppression was ~20% and ~25% respectively in global and RyR2-microdomaine in rapidly and ~35% and ~45% respectively in global and RyR2-microdomaine in slowly-inactivating cells. Conclusions:Variability in I Ca inactivation kinetics rather than cellular ancestry seems to underlie the action potential morphology differences generally attributed to mixed atrial and ventricular cell populations in hiPSC-CMs cultures. The differential hypoxic regulation of Ca 2+signaling in the two-cell types arises from differential Ca 2+ -dependent inactivation of the Ca 2+channel caused by proximity of Ca 2+ -release stores to the Ca 2+ channels.

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The effect of amphotericin B on the permeability of lipid bilayers to divalent trace metals

Cited by 19 publications

References 11 publications

Regulation of Ca2+ signaling by acute hypoxia and acidosis in rat neonatal cardiomyocytes

Regulation of Ca2+ signaling by acute hypoxia and acidosis in rat neonatal cardiomyocytes

Electrophysiological and morphological features underlying neurotransmission efficacy at the splanchnic nerve-chromaffin cell synapse of bovine adrenal medulla

Regulation of Ca2+ signaling by acute hypoxia and acidosis in cardiomyocytes derived from human induced pluripotent stem cells

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