The effect of an enzymatic bone processing method on short tandem repeat profiling of challenged bone specimens

Li, Richard; Klempner, Stacey

doi:10.1016/j.legalmed.2012.12.002

Cited by 4 publications

(6 citation statements)

References 18 publications

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“…This approach may help to better identify the root cause(s) of particular DNA typing limitations, eg, the extraction method. The next CE could also address the cleaning and grinding phase and the removal of humic acid inhibitors and modern DNA contaminants (18,33-41). Another improvement of the CE concept would be the inclusion of massive parallel sequencing (42,43) during the verification of the sample by the organizing laboratory.…”

Section: Discussionmentioning

confidence: 99%

Results of a collaborative study on DNA identification of aged bone samples

et al. 2017

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AimA collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples.MethodsEighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory.ResultsAlthough the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality.ConclusionThe laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.

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Section: Discussionmentioning

confidence: 99%

Results of a collaborative study on DNA identification of aged bone samples

et al. 2017

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“…If the strength of the fire has been increased using fire accelerants or perhaps by the combustion of some highly combustible material in the vicinity and the bodies of the deceased have been carbonized, the soft tissue may have been destroyed and the bones severely burnt and moreor-less disintegrated. In this case, genetic analysis may be possible using the lesser burned areas and/or better preserved bones or teeth, although it is important to take into consideration during the sample-gathering process for nuclear or mitochondrial DNA analysis [39][40][41], the avoidance of areas of potential contamination which could preclude successful genetic examination, e.g., areas burnt with an accelerant, as well as to specifically locate gathering areas which may potentially contain usable information that is difficult or near impossible to visually detect.…”

Section: Burnt Human Remainsmentioning

confidence: 99%

Most Common Medico-Legal Autopsy-Related Human and Nonhuman Biological Samples for DNA Analysis

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The identification and individualization of biological evidences is crucial to actual criminal investigations. In spite of the differences at the national level, all the legal processes attribute particular importance to forensic DNA analysis. However, none of the qualified results from any professional laboratory can produce substantive, valuable evidence with insufficient quality of samples and/or problems with provision of a pristine and controlled environment. The methodology and efficiency of sampling are distinct in case of living persons and in medico-legal autopsy and crime scenes. This chapter is a short overview from the basic introductory information up to ongoing research, and in accordance with constraints on the chapter size, it briefly discusses the important topics of sample collection at medico-legal autopsy for DNA analysis. The content sorts the major types of samples, reviews the common methods of sampling and the potential risk of poor sampling or contamination transfer. The corpses can be more or less degraded, which in special cases (e.g., paraffin embedded tissues, drowned, burning and/or buried cadaver) allow only for analysis of highly degraded samples. The samples can be associated with tissues of a corpse (e.g., blood, soft tissues, bone, tooth, hair) and/or additional extraneous tissues and remains, which are often mixed (e.g., blood, saliva, semen, vaginal fluid, debris of fingernails) on the corpse.

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“…An enzymatic method, using a proteolytic trypsin enzyme to degrade various types of proteins [6,7], has been utilized in the maceration of bone samples in skeletal preparation [8,9]. In our previous study, the trypsin maceration technique was adapted to prepare samples prior to DNA isolation from human fresh bone samples [10,11] and human burial bone samples [12]. Additionally, the effects of this technique on the yield of DNA isolated were compared to that of a mechanical method [12].…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, the trypsin maceration technique was adapted to prepare samples prior to DNA isolation from human fresh bone samples [10,11] and human burial bone samples [12]. Additionally, the effects of this technique on the yield of DNA isolated were compared to that of a mechanical method [12]. Comparable values of DNA yields between the two methods were observed [12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human Hair Analysis among Four Different Castes Having Potential Application in Forensic Investigation

Gaud²,

Nair³

2014

J Forensic Res

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Forensic analysis of DNA from non-human bones can be important in investigating a variety of forensic cases. However, decomposed bone is difficult to process for isolating DNA. In this study, a previously established enzymatic method was utilized to process bone samples that simulate decomposed specimens. Our results demonstrated that this enzymatic processing method is effective for removing decomposed soft tissues and outer surface materials such as mineralized bone connective tissue of bone fragment samples. Our data suggested that this method can be used in the initial sample preparation for cleaning the outer surface of decomposed non-human skeletal fragments. This study introduced an alternative method for processing decomposed non-human bone evidence prior to DNA isolation. Such a method can potentially be used to process various samples of different sizes and conditions for the investigation of a wide variety of criminal cases involving animals.

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The effect of an enzymatic bone processing method on short tandem repeat profiling of challenged bone specimens

Cited by 4 publications

References 18 publications

Results of a collaborative study on DNA identification of aged bone samples

Results of a collaborative study on DNA identification of aged bone samples

Most Common Medico-Legal Autopsy-Related Human and Nonhuman Biological Samples for DNA Analysis

Human Hair Analysis among Four Different Castes Having Potential Application in Forensic Investigation

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