The Effect of Anthelmintic Triclabendazole on Survival and Development of Galleria mellonella (Lepidoptera: Pyralidae) L. Reared on Artificial Diet

Kılıç, Ali Yavuz; Büyükgüzel, Kemal; Büyükgüzel, Ender

doi:10.9775/kvfd.2015.13731

Cited by 4 publications

(1 citation statement)

References 18 publications

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“…It is known that the effects of various anthelmintic chemicals such as niclosamide, oxyclozanide from the salicylanilide group, and triclabendazole, mebendazole, oxfendazole from the benzimidazole group on insects were investigated (Büyükgüzel and Kayaoğlu 2014, Kılıç et al 2015, Çalık et al 2016, Sugeçti et al 2016, Sefer and Büyükgüzel 2018, Çelik et al 2019). Apart from these, there have been also many studies showing the effects of various pesticides, including piperazine, on honey bees.…”

Section: Discussionmentioning

confidence: 99%

Oxidative and genotoxic effect of piperazine on Galleria mellonella (Lepidoptera: Pyralidae) hemolymph

Alkan

ÇELİK

Büyükgüzel

et al. 2023

Environmental Entomology

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Recently, there are many studies suggesting antibacterial, antifungal, and anthelmintic agents as alternative chemicals to insecticides. In this study, the oxidative and genotoxic effect of Piperazine, a clinically important hexahydropyrazine anthelmintic, on Galleria mellonella L. hemolymph tissue by adding artificial diet were investigated. Galleria mellonella larvae were reared until 7th larval stage in artificial diet containing 0.001, 0.01, 0.1, and 1 g piperazine per 100 g of diet. Using hemolymph collected from 7th-instar larvae, the amount of lipid peroxidation final product malondialdehyde (MDA), protein oxidation product protein carbonyl (PCO), and detoxification enzymes glutathione S-transferase (GST) and cytochrome P450 monooxygenase (cyt P450) activity, comet assay were measured. According to the results obtained, when the piperazine high concentrations tested with the control group were compared, statistically significant differences were found in MDA, PCO content, cyt P450, GST activity, and comet assay in the hemolymph of the insect. While MDA content was 0.01 ± 0.0021 nmol/mg protein in the control group, this amount increased approximately 2-fold at the highest concentration (0.0231 ± 0.0050 nmol/mg protein). On the other hand, when the control group and the highest piperazine concentration were compared in the GST and cyt P450 activity, it was determined that there was a statistically significant increase. We obtained similar results in comet assay and micronucleus formation data. This study showed that the tested piperazine concentrations caused significant changes in the detoxification capacity, oxidative stress, and genotoxic markers in the insect’s hemolymph tissue.

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Section: Discussionmentioning

confidence: 99%

Oxidative and genotoxic effect of piperazine on Galleria mellonella (Lepidoptera: Pyralidae) hemolymph

Alkan

ÇELİK

Büyükgüzel

et al. 2023

Environmental Entomology

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Effects of zinc oxide nanoparticles (ZnO NPs) on the biology of Galleria mellonella L. (Lepidoptera: Pyralidae)

Eskin

Nurullahoğlu

2022

JoBAZ

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Background Because of its ability to absorb UV radiation and possess catalytic, antibacterial, and semiconducting properties, zinc oxide nanoparticles (ZnO NPs) are increasingly being used in consumer goods. Because nanoparticles are used so often, accurate methods for determining any associated toxicity are crucial. The greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae) is a suitable experimental model organism due to immune defense of the larvae is very strong. Also, larvae are a good model for carrying out toxicity studies. In this study, to determine the effects of zinc oxide NPs (ZnO NPs) on the biology (larval development time, the weight of the last stage larvae, the pupal development time, the pupal weight, the eclosion rate, the maturation period, the adult weights, the adult longevity, and the percentage of adults) of G. mellonella, zinc oxide NPs were added to the larvae diet (honeycomb) at different doses (100, 500, 1000, 3000, and 5000 ppm). Results Results showed that pupal weights significantly decreased at 1000, 3000, and 5000 ppm doses of zinc oxide NPs when compared with control. Adult weight increased at 100 ppm zinc oxide NPs and the adult longevity of G. mellonella in the group exposed to 5000 ppm zinc oxide NP was longer than the control group. Finally, total male longevity extended in 500, 1000, 3000, and 5000 ppm zinc oxide NPs groups when compared to the control group. Conclusions The findings of this study contribute to evidence that the negative effects of ZnO NPs on biological properties on G. mellonella. In addition, the study reveals the adverse effects of zinc oxide NPs on a model experimental organism and provides an idea for researchers working on this subject in terms of new studies that can be done in future.

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Penicillin Impact on Survivorship, Development, and Adult Longevity of Drosophila melanogaster (Diptera: Drosophilidae)

Üstündağ

Büyükgüzel

2020

Journal of Entomological Science

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Survivorship, developmental period, and adult longevity of the fruit fly, Drosophila melanogaster Meigen (Diptera: Drosophilidae), exposed to penicillin G, a beta-lactam antibiotic, was determined in laboratory testing. In the study, neonate larvae were placed and reared on dietary antibiotics at concentrations of 100, 200, 400, or 800 mg/L. All penicillin concentrations significantly decreased survivorship in an inverse relationship with third instars, pupae, and adults. Larvae fed on control diets of 0 mg/L of penicillin had a survival level of 91.00 ± 3.27% to third instar and pupation, and 89.00 ± 4.09% to adult emergence. Feeding on a diet containing the highest concentration of penicillin (800 mg/L) significantly decreased survivorship to third instar to 11.00 ± 2.59% (χ2 = 128.051; df = 1; P = 0.0001) and to pupation (χ2 = 131.233; df = 1; P = 0.0001) and adult emergence to approximately 10.00 ± 1.00% (χ2 = 124.832; df = 1; P = 0.0001). The highest concentration of dietary penicillin (800 mg/L) also significantly prolonged developmental time from neonate to third instars by 1.5 d (F = 17.229; df = 4; P = 0.0001) and from neonate to adult emergence by 3 d (F = 2.004; df = 4; P = 0.032). Compared to the control group, adult longevity was significantly reduced by the antibiotic in a dose-related manner. The use of this antibiotic in insect artificial rearing enables an extensive search of possible insecticidal action of penicillin with high dietary levels for agricultural purposes.

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The Effect of Anthelmintic Triclabendazole on Survival and Development of Galleria mellonella (Lepidoptera: Pyralidae) L. Reared on Artificial Diet

Cited by 4 publications

References 18 publications

Oxidative and genotoxic effect of piperazine on Galleria mellonella (Lepidoptera: Pyralidae) hemolymph

Oxidative and genotoxic effect of piperazine on Galleria mellonella (Lepidoptera: Pyralidae) hemolymph

Effects of zinc oxide nanoparticles (ZnO NPs) on the biology of Galleria mellonella L. (Lepidoptera: Pyralidae)

Penicillin Impact on Survivorship, Development, and Adult Longevity of Drosophila melanogaster (Diptera: Drosophilidae)

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