The Effect of Anti-coagulants on the Osmotic Fragility of Erythrocytes in the Yak(Poephagus grunniens)

Sarkar, Mihir; Barari, S. K.; Mandal, D.; Nandankar, U. A.; Basu, Avik; Mohanty, T.K.; Ray, Swati

doi:10.1053/tvjl.1998.0257

Cited by 14 publications

(10 citation statements)

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“…Esse fato possivelmente ocorre devido à quelação dos íons Ca 2+ que determina distúrbios na permeabilidade e estabilidade da membrana dos eritrócitos (WALENCIK & WITESKA, 2007). SARKAR et al (1999) citaram que a fragilidade osmótica dos eritrócitos é um teste que determina a resistência destes frente ao estresse osmótico. O início da hemólise no presente estudo foi verificado a partir da concentração de 0,45% de NaCl-PO 4 no grupo controle e de heparina, não havendo diferença entre estes.…”

Section: Resultsunclassified

Heparina e Na2EDTA como anticoagulantes para surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans): eficácia e alterações hematológicas

et al. 2010

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Section: Resultsunclassified

Heparina e Na2EDTA como anticoagulantes para surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans): eficácia e alterações hematológicas

et al. 2010

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“…Erythrocyte osmotic fragility (EOF) was measured following established protocols [ 66 ]. A series of saline solutions were prepared to provide NaCl concentrations of 0.00, 0.10, 0.20, 0.40, 0.60, 0.80, and 0.85% w/v in distilled water.…”

Section: Methodsmentioning

confidence: 99%

Uncoupling Thermotolerance and Growth Performance in Chinook Salmon: Blood Biochemistry and Immune Capacity

et al. 2021

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Ocean warming and extreme sea surface temperature anomalies are threatening wild and domesticated fish stocks in various regions. Understanding mechanisms for thermotolerance and processes associated with divergent growth performance is key to the future success of aquaculture and fisheries management. Herein, we exposed Chinook salmon (Oncorhynchus tshawytscha) to environmentally relevant water temperatures (19–20 °C) approaching their upper physiological limit for three months and sought to identify blood biomarkers associated with thermal stress and resilience. In parallel, blood biochemical associations with growth performance were also investigated. Temperature stress-activated leukocyte apoptosis induced a minor immune response, and influenced blood ion profiles indicative of osmoregulatory perturbation, regardless of how well fish grew. Conversely, fish displaying poor growth performance irrespective of temperature exhibited numerous biomarker shifts including haematology indices, cellular-based enzyme activities, and blood clinical chemistries associated with malnutrition and disturbances in energy metabolism, endocrine functioning, immunocompetence, redox status, and osmoregulation. Findings provide insight into mechanisms of stress tolerance and compromised growth potential. Biochemical phenotypes associated with growth performance and health can potentially be used to improve selective breeding strategies.

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“…The evaluation of the osmotic fragility of erythrocytes shows the resistance of these cells in front of osmotic stress simulated by different NaCl concentration solutions, and the degree of hemolysis is measured by the amount hemoglobin liberated by the rupture of the cells (Sarkar et al 1999). Blood samples of pacus stored with Na 2 EDTA 10 % presented increase in the cell lysis between 0.35 to 0.65% NaCl-PO 4 solution.…”

Section: Discussionmentioning

confidence: 99%

Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus

Farias¹,

Pereira²,

Pádua³

et al. 2016

Pesq. Vet. Bras.

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RESUMO.-[Anticoagulante Na 2 EDTA danifica amostras de sangue do teleósteo Piaractus mesopotamicus.] O presente estudo teve por objetivo avaliar os efeitos da heparina sódica e Na 2 EDTA no sangue de Piaractus mesopotamicus (360,7±42,4g, 26,4±1,0cm). Foram amostrados vinte peixes em dois ensaios experimentais, sendo dez peixes utilizados para análise da fragilidade dos eritrócitos e dez peixes para análise dos parâmetros hematológicos e estudo bioquímico do plasma. O sangue coletado por punção veno-caudal foi aliquotado e armazenado em diferentes soluções anticoagulantes: Na 2 EDTA 10%, Na 2 EDTA 3%, heparina sódica 5.000 UI e heparina sódica 100 UI. Níveis plasmáticos de cálcio apresentados nas amostras armazenados em Na 2 EDTA diminuíram cerca de 80% em relação aos dois grupos armazenados com heparina. Amostras de sangue de pacus armazenados com Na 2 EDTA demonstraram aumento do hematócrito e VCM, e diminuição na CHCM. O efeito dose-resposta foi observado neste estudo. Estes resultados são reforçados pelos níveis mais elevados de proteína plasmática e hemólise apresentado no sangue armazenado com Na 2 EDTA 10%, o que confirma o efeito deletério desse tratamento anticoagulante na qualidade de amostras de sangue. Na 2 EDTA não é indicada para armazenar amostras de sangue de P. mesopotamicus, e heparina sódica a 100 UI é o anticoagulante mais recomendado, uma vez que este tratamento apresentou a menor taxa de alterações no sangue armazenado. The present study aimed to evaluate the effects of Na heparin and Na 2 EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na 2 EDTA 10%, Na 2 EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na 2 EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na 2 EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na 2 EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na 2 EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood. INDEX TERMS: Fish hematology, blood clotting, EDTA, Heparin, Piaractus mesopotamicus.

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The Effect of Anti-coagulants on the Osmotic Fragility of Erythrocytes in the Yak(Poephagus grunniens)

Cited by 14 publications

References 0 publications

Heparina e Na2EDTA como anticoagulantes para surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans): eficácia e alterações hematológicas

Heparina e Na2EDTA como anticoagulantes para surubim híbrido (Pseudoplatystoma reticulatum x P. corruscans): eficácia e alterações hematológicas

Uncoupling Thermotolerance and Growth Performance in Chinook Salmon: Blood Biochemistry and Immune Capacity

Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus

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