The effect of antibiotics on the in vivo synthesis of messenger ribonucleic acid from the lactose operon of Escherichia coli

Dütting, D.; Hübner, L.

doi:10.1007/bf00269771

Cited by 17 publications

(12 citation statements)

References 36 publications

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“…The abrupt decrease in the rate of DNA replication observed after inhibition of translation by CAM or by removal of required amino acids can also be explained by a slowing of fork movement resulting from transient immobilization of transcription complexes on the DNA template. Addition of CAM prevents movement of ribosomes along nascent mRNA but allows continued synthesis of mRNA (10,21). In the absence of ribosomes on the nascent mRNA, DNA-RNA complexes (e.g., see reference 3) may persist for a time sufficient to impede the progression of DNA replication forks.…”

Section: Resultsmentioning

confidence: 99%

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Alterations of the rate of movement of deoxyribonucleic acid replication forks

Pato¹

1975

J Bacteriol

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Antibiotics that inhibit ribonucleic acid (RNA) or protein synthesis are often used in studies of deoxyribonucleic acid (DNA) synthesis. The experiments presented here demonstrate that the rate of movement of DNA replication forks can be influenced by such antibotics. Addition of either chloramphenicol, which inhibits movement of ribosomes along messenger RNA, or streptolydigin, which inhibits movement of RNA polymerase, leads to a decrease in the rate of fork movement. Rifampin, which inhibits initiation of RNA synthesis, reverses the effects of chloramphenicol or streptolydigin. The slowed movement of DNA replication forks is discussed in terms of obstruction of fork movement by transcription complexes temporarily immobilized on the DNA template.

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Section: Resultsmentioning

confidence: 99%

“…[3H]thymidine (10 MCi/ml) was added 5 min before antibiotics. CAM (A, 200 Mg/mi) or RIF (0, 200 Mg/mO) was added at zero time; the control culture received no antibiotics (0).…”

Section: Resultsmentioning

confidence: 99%

Alterations of the rate of movement of deoxyribonucleic acid replication forks

Pato¹

1975

J Bacteriol

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“…Early models (27) postulated that ribosome movement is necessary for transcription to occur, but it is now clear that transcription can take place in the absence of translation. This point has been demonstrated by in vivo experiments in which antibiotics or amino acid starvation were used to inhibit translation (6,11,17,28) without abolishing transcription. The fact that messenger ribonucleic acids (mRNA) can be synthesized in vitro also demonstrates the lack of any obligatory coupling between transcription and translation (5,7).…”

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confidence: 96%

Messenger Ribonucleic Acid Synthesis and Degradation in Escherichia coli During Inhibition of Translation

1973

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Various aspects of the coupling between the movement of ribosomes along messenger ribonucleic acids (mRNA) and the synthesis and degradation of mRNA have been investigated. Decreasing the rate of movement of ribosomes along an mRNA does not affect the rate of movement of some, and possibly most, of the RNA polymerases transcribing the gene coding for that mRNA. Inhibiting translation with antibiotics such as chloramphenicol, tetracycline, or fusidic acid protects extant mRNA from degradation, presumably by immobilizing ribosomes, whereas puromycin exposes mRNA to more rapid degradation than normal. The promoter distal (3′) portion of mRNA, synthesized after ribosomes have been immobilized by chloramphenicol on the promoter proximal (5′) portion of the mRNA, is subsequently degraded.

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“…This would indicate that the postulated RNAdependent mechanism that functions during CAP treatment is nonfunctional during amino acid starvation. This could be due to a drastic reduction in the rate of synthesis of ribosomal or transfer RNA during amino acid starvation (4, 11) (as opposed to the acceleration in rate of synthesis during CAP treatment [see reference 9]) or to failure of messenger RNA to accumulate during AA starvation (7,8) (as it does during CAP treatment [see references 6,15]).…”

Section: Discussionmentioning

confidence: 99%

“…Since the net increase in deoxyribonucleic acid (DNA) was shown to be identical under these two conditions of inhibited protein synthesis, it is evident that some factor other than chromosomal replication is implicated in the production of increased UVresistance in the Hcr-strain. Ribonucleic acid (RNA) metabolism differs significantly in CAPtreated (6,9,15) and in amino acid-starved (4,7,8,11) E. coli. This difference could be the reason for the difference in UV resistance attained during the two preirradiation treatments.…”

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confidence: 99%

Effect of Preirradiation Ribonucleic Acid Synthesis Inhibition on Resistance to Ultraviolet Light with Resistant and Sensitive Strains of Escherichia coli B/r

Rudé

Doudney

1974

J Bacteriol

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The ultraviolet resistance of a streptolydigin-susceptible strain of Escherichia coli B/r hcr-increased during preirradiation treatment with streptolydigin (an inhibitor of deoxyribonucleic acid-dependent ribonucleic acid polymerase) for 20 min and then remained constant. During preirradiation treatment with chloramphenicol (an inhibitor of protein synthesis), resistance to ultraviolet light increased for 1 to 2 h, and reached a maximal level significantly above that attained in streptolydigin-containing medium. These results suggest that there are two mechanisms that function in Hcr-cells during chloramphenicol treatment which contribute to the concomitant ultraviolet resistance enhancement. One is ribonucleic acid dependent and is inhibited by streptolydigin. This ribonucleic acid-dependent mechanism appears to be absent in wild-type and RecA E. coli B/r strains.Experimental design and UV irradiation procedure. The logarithmic phase culture was either prepared immediately for UV irradiation or was subjected to onq of the following preirradiation treat-123 on August 1, 2020 by guest

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The effect of antibiotics on the in vivo synthesis of messenger ribonucleic acid from the lactose operon of Escherichia coli

Cited by 17 publications

References 36 publications

Alterations of the rate of movement of deoxyribonucleic acid replication forks

Alterations of the rate of movement of deoxyribonucleic acid replication forks

Messenger Ribonucleic Acid Synthesis and Degradation in Escherichia coli During Inhibition of Translation

Effect of Preirradiation Ribonucleic Acid Synthesis Inhibition on Resistance to Ultraviolet Light with Resistant and Sensitive Strains of Escherichia coli B/r

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