The Effect of Antimicrobial Peptide Temporin-Ra on Cell Viability and Gene Expression of Pro-inflammatory Factors in A549 Cell Line

Asadi, Fateme; Asoodeh, Ahmad; Kashef, Reyhaneh; Housaindokht, Mohammad Reza; Haghparast, Alireza; Chamani, Jamshidkhan

doi:10.1007/s10989-013-9363-y

Cited by 7 publications

(1 citation statement)

References 24 publications

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“…Absorbance at 570 nm was determined using a 96‐well ELISA plate reader (ELX800 ELISA reader; Bio‐Tek Instruments, Winooski, VT, USA). Triplicate readings from three independent assays were averaged, and the cell viability (%) after treatment with neuropeptide was calculated against the control nontreated wells based on the following formula :

Viability (italic%) = (average OD treated cells) / (average OD control cells) \times 100

…”

Section: Methodsmentioning

confidence: 99%

Antioxidant properties of a human neuropeptide and its protective effect on free radical‐induced DNA damage

Mohseni

Emtenani

et al. 2014

Journal of Peptide Science

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Human catestatin CgA352-372 (SL21) is an endogenous neuropeptide with multiple biological functions. The present study aimed to evaluate the antioxidant, antibacterial, cytotoxic, and DNA damage protective effects of SL21 neuropeptide. SL21 neuropeptide generated from the C-terminus of chromogranin A (CgA) was synthesized by solid-phase method. Synthetic peptide was subjected to various in vitro antioxidant assays including the scavenging of 1,1-diphenyl-2-pycryl-hydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS(·+) ), and hydroxyl free radicals, metal ion chelation, inhibition of lipid peroxidation, and reducing power. Moreover, protective effect of SL21 on H2 O2 -induced DNA damage was analyzed using pTZ57/RT plasmid. Methylthiazoltetrazolium assay was also performed to study the cytotoxic effect of SL21 neuropeptide on human peripheral blood mononuclear cells. Furthermore, antibacterial and hemolysis assays were conducted. The results demonstrated high activities of SL21 in scavenging free radicals (DPPH, ABTS(·+) , and hydroxyl), chelating of Cu(2+) /Fe(2+) metal ions, reducing power, and inhibition of lipid peroxidation in a concentration-dependent manner. SL21 neuropeptide revealed a protective effect on DNA damage caused by hydroxyl radicals. Interestingly, the peptide exhibited no significant cytotoxicity towards peripheral blood mononuclear cells. Furthermore, SL21 peptide displayed antimicrobial activity against Staphylococcus aureus and Pseudomonas aeruginosa without any hemolytic activity on human red blood cells. Conclusively, the present study established SL21 (catestatin) as a novel antioxidative peptide that could further be investigated for its potential use as a pharmaceutical agent.

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