The effect of antimicrobial photodynamic therapy using yellow-green LED and rose bengal on Porphyromonas gingivalis

Kitanaka, Yutaro; Takeuchi, Yasuo; Hiratsuka, Koichi; Aung, Nay; Sakamaki, Yuriko; Nemoto, Takashi; Meinzer, Walter; Izumi, Yuichi; Iwata, Takanori; Aoki, Akira

doi:10.1016/j.pdpdt.2020.102033

Cited by 11 publications

(4 citation statements)

References 33 publications

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“…The maximum absorption wavelength of RB is approximately 550 nm. Therefore, Kitanaka et al [80] investigated the antibacterial effects of a-PDT using a new combination of yellow-green LED light (565 nm) and RB against P. gingivalis in vitro. The results showed that a-PDT with yellow-green LED (8.56 J/cm 2 ) and RB showed higher antibacterial activity against P. gingivalis than a-PDT with blue LED light (470 nm, 8.56 J/cm 2 ) and RB.…”

Section: Green/yellow Lightmentioning

confidence: 99%

Application of Different Wavelengths of LED Lights in Antimicrobial Photodynamic Therapy for the Treatment of Periodontal Disease

Takeuchi,

Aoki,

Hiratsuka

et al. 2023

Antibiotics

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Therapeutic light has been increasingly used in clinical dentistry for surgical ablation, disinfection, bio-stimulation, reduction in inflammation, and promotion of wound healing. Photodynamic therapy (PDT), a type of phototherapy, has been used to selectively destroy tumor cells. Antimicrobial PDT (a-PDT) is used to inactivate causative bacteria in infectious oral diseases, such as periodontitis. Several studies have reported that this minimally invasive technique has favorable therapeutic outcomes with a low probability of adverse effects. PDT is based on the photochemical reaction between light, a photosensitizer, and oxygen, which affects its efficacy. Low-power lasers have been predominantly used in phototherapy for periodontal treatments, while light-emitting diodes (LEDs) have received considerable attention as a novel light source in recent years. LEDs can emit broad wavelengths of light, from infrared to ultraviolet, and the lower directivity of LED light appears to be suitable for plaque control over large and complex surfaces. In addition, LED devices are small, lightweight, and less expensive than lasers. Although limited evidence exists on LED-based a-PDT for periodontitis, a-PDT using red or blue LED light could be effective in attenuating bacteria associated with periodontal diseases. LEDs have the potential to provide a new direction for light therapy in periodontics.

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Section: Green/yellow Lightmentioning

confidence: 99%

Application of Different Wavelengths of LED Lights in Antimicrobial Photodynamic Therapy for the Treatment of Periodontal Disease

Takeuchi,

Aoki,

Hiratsuka

et al. 2023

Antibiotics

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“…Antimicrobial photodynamic therapy (aPDT) is known to eliminate bacterial infectious agents using reactive oxygen species (ROS) generated by irradiating a photosensitizer (PS) with a light source, particularly laser, of the same excitation wavelength. In the field of dentistry, aPDT is currently being studied for use to treat periodontal diseases [ 1 , 2 , 3 ], endodontics [ 4 , 5 ], and peri-implantitis [ 3 , 6 , 7 ], as well as dental caries [ 8 , 9 , 10 ]. The mechanism of action of aPDT is that, when PS is excited by laser irradiation at a specific wavelength, it forms an excited triplet state, which transfers energy to oxygen molecules to form highly cytotoxic ROS such as hydroxyl radicals and singlet oxygen [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Laser Irradiation Modes and Photosensitizer Types on Antimicrobial Photodynamic Therapy (aPDT) for Streptococcus sobrinus in the Crown Dentin of Bovine Teeth: An Experimental In Vitro Study

Yamaguchi,

Yoshii,

Katsuragi

et al. 2024

Dentistry Journal

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This study aimed to assess the impact of different laser irradiation modes and photosensitizer types on the bactericidal efficacy of antimicrobial photodynamic therapy (aPDT). Dentin plates were prepared by sectioning the crown dentin of bovine teeth infected with Streptococcus sobrinus (n = 11). Nine aPDTs involving the combination of three 1% solutions of photosensitizers (brilliant blue, BB; acid red, AR; and methylene blue, MB) and three irradiation modes of semiconductor lasers (50 mW for 120 s, 100 mW for 60 s, and 200 mW for 30 s) were performed for each infected dentin plate, and the control consisted of the specimens not applied with aPDT. The bactericidal effects in 10 groups were evaluated using both assays of the colony count (colony-forming-unit: CFU) and adenosine triphosphate (ATP) (relative-light-unit: RLU). The data obtained were analyzed using the Kruskal–Wallis test (α = 0.05). The most aPDT groups exhibited significantly lower RLU and CFU values compared with the control (p < 0.05). The effect of irradiation modes on RLU and CFU values was significant in the aPDT group using BB (p < 0.05) but not in the aPDT group using AR or MB. The aPDT performed with AR or MB exerted a remarkable bactericidal effect.

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“…In recent years the photodynamic activity of bengal rose activated with blue or green light against selected bacterial strains and fungi has been reported (de Oliveira Silva et al, 2023;Hung et al, 2022;Kitanaka et al, 2020;Wang et al, 2021). However, according to our knowledge, the differences between the sensitivity of Gram-positive and Gram-negative bacterial strains in the presence of this photosensitiser activated with green light have not described.…”

Section: Introductionmentioning

confidence: 99%

The effect of rose bengal activated with green diode laser light on selected Gram-positive and Gram-negative bacterial strains

Greczek-Stachura,

Różanowski,

Kania

2023

AUPCStudiaNaturae

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In recent years the photodynamic activity of rose bengal activated with green light against selected bacterial strains has been reported. However, according to our knowledge, the differences between the sensitivity of Gram-positive and Gram-negative bacterial strains in the presence of this photosensitizer have not been described. The aim of the conducted research was to examine the antibacterial effect of 535 nm wavelength diode laser light in the presence of rose bengal as photosensitizer on selected reference bacterial strains: Pseudomonas aeruginosa, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae and Staphylococcus aureus. Sterile 96-well microtiter plates were used to determine the antibacterial activity of the green light and rose bengal solutions at various concentrations. The labelled bacterial suspensions were placed to each well of the 96-well microtiter plate filled with liquid medium LB and solution of rose bengal. The plates were exposed to green diode laser light. After 24 hours of incubation at 37oC, the turbidance was read in a spectrophotometer. The irradiation in the presence of photosensitizer can act in an antibacterial manner, either bacteriostatically or bactericidally. The tested strains exhibit different sensitivity to irradiation because of the structure of the cell wall, the presence of different bacterial pigments and photoreceptor proteins in some species of bacteria. Gram-positive bacteria, Staphylococcus aureus and Enterococcus faecalis were the most photosensitive strains due to the higher possibility of rose bengal penetration into the bacterial cell, leading to the bacteriostatic effect. Our results show that rose bengal may be applied in the treatment of Gram-positive infections.

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The effect of antimicrobial photodynamic therapy using yellow-green LED and rose bengal on Porphyromonas gingivalis

Cited by 11 publications

References 33 publications

Application of Different Wavelengths of LED Lights in Antimicrobial Photodynamic Therapy for the Treatment of Periodontal Disease

Application of Different Wavelengths of LED Lights in Antimicrobial Photodynamic Therapy for the Treatment of Periodontal Disease

Effect of Laser Irradiation Modes and Photosensitizer Types on Antimicrobial Photodynamic Therapy (aPDT) for Streptococcus sobrinus in the Crown Dentin of Bovine Teeth: An Experimental In Vitro Study

The effect of rose bengal activated with green diode laser light on selected Gram-positive and Gram-negative bacterial strains

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