The Effect of Antioxidant Added to Preservation Solution on the Protection of Kidneys before Transplantation

Ostróżka-Cieślik, Aneta

doi:10.3390/ijms23063141

Cited by 9 publications

(6 citation statements)

References 141 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Particularly, oxygen carriers have been applied experimentally in kidney preservation. At the same time, the addition of free radical scavengers, such as superoxide dismutase (SOD), to the preservation solution has been found to be beneficial in preventing the generation of oxygen free radicals in this highly oxygenated environment [ 15 , 67 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Oxidative Stress and Metabolic Profile in a Preclinical Kidney Transplantation Model According to Different Preservation Modalities

Mrakic‐Sposta

Vezzoli

Cova

et al. 2023

IJMS

View full text Add to dashboard Cite

This study addresses a joint nuclear magnetic resonance (NMR) and electron paramagnetic resonance (EPR) spectroscopy approach to provide a platform for dynamic assessment of kidney viability and metabolism. On porcine kidney models, ROS production, oxidative damage kinetics, and metabolic changes occurring both during the period between organ retrieval and implantation and after kidney graft were examined. The 1H-NMR metabolic profile—valine, alanine, acetate, trimetylamine-N-oxide, glutathione, lactate, and the EPR oxidative stress—resulting from ischemia/reperfusion injury after preservation (8 h) by static cold storage (SCS) and ex vivo machine perfusion (HMP) methods were monitored. The functional recovery after transplantation (14 days) was evaluated by serum creatinine (SCr), oxidative stress (ROS), and damage (thiobarbituric-acid-reactive substances and protein carbonyl enzymatic) assessments. At 8 h of preservation storage, a significantly (p < 0.0001) higher ROS production was measured in the SCS vs. HMP group. Significantly higher concentration data (p < 0.05–0.0001) in HMP vs. SCS for all the monitored metabolites were found as well. The HMP group showed a better function recovery. The comparison of the areas under the SCr curves (AUC) returned a significantly smaller (−12.5 %) AUC in the HMP vs. SCS. EPR-ROS concentration (μmol·g−1) from bioptic kidney tissue samples were significantly lower in HMP vs. SCS. The same result was found for the NMR monitored metabolites: lactate: −59.76%, alanine: −43.17%; valine: −58.56%; and TMAO: −77.96%. No changes were observed in either group under light microscopy. In conclusion, a better and more rapid normalization of oxidative stress and functional recovery after transplantation were observed by HMP utilization.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of Oxidative Stress and Metabolic Profile in a Preclinical Kidney Transplantation Model According to Different Preservation Modalities

Mrakic‐Sposta

Vezzoli

Cova

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Thus, the addition of dextran to the perfusate may contribute to healthy vasculature and stable organ functionality ( 55 ). Antioxidants and anti-inflammatory molecules have also been under extensive investigation as supplements since they scavenge reactive oxygen species (ROS) arising from IRI and dampen the immunological response ( 78 ). As such, various agents including vitamin C, quercetin, and resveratrol have shown beneficial effects ( 79 , 80 ).…”

Section: Ex Situ Machine Perfusionmentioning

confidence: 99%

Pushing the boundaries of innovation: the potential of ex vivo organ perfusion from an interdisciplinary point of view

Iske,

Schroeter,

Knoedler

et al. 2023

Front. Cardiovasc. Med.

View full text Add to dashboard Cite

Ex vivo machine perfusion (EVMP) is an emerging technique for preserving explanted solid organs with primary application in allogeneic organ transplantation. EVMP has been established as an alternative to the standard of care static-cold preservation, allowing for prolonged preservation and real-time monitoring of organ quality while reducing/preventing ischemia–reperfusion injury. Moreover, it has paved the way to involve expanded criteria donors, e.g., after circulatory death, thus expanding the donor organ pool. Ongoing improvements in EVMP protocols, especially expanding the duration of preservation, paved the way for its broader application, in particular for reconditioning and modification of diseased organs and tumor and infection therapies and regenerative approaches. Moreover, implementing EVMP for in vivo-like preclinical studies improving disease modeling raises significant interest, while providing an ideal interface for bioengineering and genetic manipulation. These approaches can be applied not only in an allogeneic and xenogeneic transplant setting but also in an autologous setting, where patients can be on temporary organ support while the diseased organs are treated ex vivo, followed by reimplantation of the cured organ. This review provides a comprehensive overview of the differences and similarities in abdominal (kidney and liver) and thoracic (lung and heart) EVMP, focusing on the organ-specific components and preservation techniques, specifically on the composition of perfusion solutions and their supplements and perfusion temperatures and flow conditions. Novel treatment opportunities beyond organ transplantation and limitations of abdominal and thoracic EVMP are delineated to identify complementary interdisciplinary approaches for the application and development of this technique.

show abstract

“…In addition, well-known hydrosoluble antioxidants (including vitamin C, N-acetyl cysteine (NAC), alpha-tocopherol analog, and others) were used as additives to preservation solutions to reinforce their respective antioxidant features [ 54 , 55 , 56 , 57 ]. Further investigations are needed to explore new antioxidants for graft preservation purposes.…”

Section: New Additives For Improving Cold Static Preservation: Oxygen...mentioning

confidence: 99%

Liver Graft Hypothermic Static and Oxygenated Perfusion (HOPE) Strategies: A Mitochondrial Crossroads

Bardallo

Silva

Carbonell

et al. 2022

IJMS

View full text Add to dashboard Cite

Marginal liver grafts, such as steatotic livers and those from cardiac death donors, are highly vulnerable to ischemia–reperfusion injury that occurs in the complex route of the graft from “harvest to revascularization”. Recently, several preservation methods have been developed to preserve liver grafts based on hypothermic static preservation and hypothermic oxygenated perfusion (HOPE) strategies, either combined or alone. However, their effects on mitochondrial functions and their relevance have not yet been fully investigated, especially if different preservation solutions/effluents are used. Ischemic liver graft damage is caused by oxygen deprivation conditions during cold storage that provoke alterations in mitochondrial integrity and function and energy metabolism breakdown. This review deals with the relevance of mitochondrial machinery in cold static preservation and how the mitochondrial respiration function through the accumulation of succinate at the end of cold ischemia is modulated by different preservation solutions such as IGL-2, HTK, and UW (gold-standard reference). IGL-2 increases mitochondrial integrity and function (ALDH2) when compared to UW and HTK. This mitochondrial protection by IGL-2 also extends to protective HOPE strategies when used as an effluent instead of Belzer MP. The transient oxygenation in HOPE sustains the mitochondrial machinery at basal levels and prevents, in part, the accumulation of energy metabolites such as succinate in contrast to those that occur in cold static preservation conditions. Additionally, several additives for combating oxygen deprivation and graft energy metabolism breakdown during hypothermic static preservation such as oxygen carriers, ozone, AMPK inducers, and mitochondrial UCP2 inhibitors, and whether they are or not to be combined with HOPE, are presented and discussed. Finally, we affirm that IGL-2 solution is suitable for protecting graft mitochondrial machinery and simplifying the complex logistics in clinical transplantation where traditional (static preservation) and innovative (HOPE) strategies may be combined. New mitochondrial markers are presented and discussed. The final goal is to take advantage of marginal livers to increase the pool of suitable organs and thereby shorten patient waiting lists at transplantation clinics.

show abstract

The Effect of Antioxidant Added to Preservation Solution on the Protection of Kidneys before Transplantation

Cited by 9 publications

References 141 publications

Evaluation of Oxidative Stress and Metabolic Profile in a Preclinical Kidney Transplantation Model According to Different Preservation Modalities

Evaluation of Oxidative Stress and Metabolic Profile in a Preclinical Kidney Transplantation Model According to Different Preservation Modalities

Pushing the boundaries of innovation: the potential of ex vivo organ perfusion from an interdisciplinary point of view

Liver Graft Hypothermic Static and Oxygenated Perfusion (HOPE) Strategies: A Mitochondrial Crossroads

Contact Info

Product

Resources

About