The effect of APN, hs-CRP and APN/hs-CRP in periodontitis with DAA

Abstract

Background: Common chronic infections induced low-grade inflammation has been correlated with atherosclerosis as supported by strong evidence. The balance between pro-and anti-inflammatory factors was exploited to elucidate the effects of chronic periodontitis on diabetes-associated atherosclerosis. Methods: Study subjects encompassed 30 SPF male rats randomly divided into four groups: A group,normal control(NC), B group, type 2 diabetes mellitus (T2DM), C group, chronic periodontitis (CP), D group (DM+CP). After developing the model, blood samples were collected from the angular vein analyze serum adiponectin (APN), high sense- c reactive protein (hs-CRP), and blood lipid. the carotid artery was isolated for HE staining. Result: Compared with group A, the serum APN in group B, C and D decreased gradually with the progression of the disease. Serum hS-CRP in group B, C and D was significantly increased. At T3, T4 and T5 in group B, C and D, APN/hs-CRP significantly decreased .TC, LDL and TG significantly increased in group B, D; HDL significantly decreased in group C. Carotid artery HE staining showed: compared with group A, different degrees of endothelial defect, destruction of elastic fibers in the middle membrane, disorder of smooth muscle arrangement, and partial dissolution 、 fragmentation and Calcium salt deposition necrosis occurred in group B, C and D. Conclusion: Enhanced systemic inflammation, decreased adiponectin level, and disorganized lipid metabolism with or without type 2 diabetes attributed to local inflammation of periodontitis can result in an imbalance of pro-inflammatory and anti-inflammatory effects. Therefore, it’s more meaningful to predict the progression of DAA with anti-inflammatory/pro-inflammatory variation.