The effect of aqueous extract of zest of citrus sinensis (AEZCs) on cadmium chloride induced liver toxicity in wistar rats

Abstract

The potency of the peels of Citrus sinensis against cadmium induced liver damage has not been explored in our environment. 48 wistar rats were used for this study. The animals were randomly divided into eight (8) groups of five (5) rats each. Group A was the positive control and received 5 mg/kg body weight (bw) of cadmium chloride (CdCl 2 ) intraperitoneally as a single dose. Groups B and C received the aqueous extract of zest of citrus sinensis (AEZCS) at a low doses of 10 and 40 mg/kg bw respectively. Groups D and E received cadmium chloride, followed by low and high doses of AEZCS respectively. Groups F and G received low and high doses of AEZCS followed by CdCl2 while group H served as the normal control. Liver enzymes (AST, ALT and ALP) and serum total proteins were analyzed. The results showed significant (P<0.05) differences in the mean values of LV/BW, ALT, AST, total proteins, serum dismutase (SOD) and malondialdehyde (MDA) when the positive control group was compared with the normal control group (P<0.05). Histological sections of the negative control groups were significantly different from the positive control group but not from the groups treated with AEZCS at the high doses. Thus, AEZCS had ameliorative and protective health benefits at the high dose of 40mg/kg body weight.

Ameliorative Effects of Oyster Protein Hydrolysates on Cadmium-Induced Hepatic Injury in Mice

“…Control group: Intraperitoneal injection of 0.9% NaCl (saline) once daily. Cd-exposed group: the mice were injected intraperitoneally with CdCl2 5 mg/kg daily [87].…”

“…Control group: Intraperitoneal injection of 0.9% NaCl (saline) once daily. Cd-exposed group: the mice were injected intraperitoneally with CdCl 2 5 mg/kg daily [87]. EDTA-treated group (positive control): the mice were injected with CdCl 2 (5 mg/kg) intraperitoneally after 1 h of oral administration with EDTA (100 mg/kg) daily.…”

Alleviative effect of threonine on cadmium-induced acute liver injury in mice

“…These SPF mice were kept in plastic cages containing sawdust at 22-25 °C under a standard 12:12 h light/dark cycle with ad libitum access to standard feed and water. As previously described [10][11][12], after adapting for one week, mice were randomly divided into 4 groups: (1) control group, intraperitoneal injection of 0.9% NaCl once a day; (2) Cd-exposed group, intraperitoneal injection of 5 mg/kg/day CdCl 2 ; (3) Cd+L-Thr group, of intraperitoneal injection of 5 mg/kg/day CdCl 2 and oral administration of 0.04 mmol/g/day of Thr;(4) Cd+H-Thr group, intraperitoneal injection of 5 mg/kg/day CdCl 2 and oral administration of 0.08 mmol /kg/day. Mice were continuously treated for 7 days, and then sacri ced.…”