The effect of arabinofuranosyl-cytosine upon the synthesis of herpesvirus hominis Electron microscopic observations in relation to viral DNA-synthesis

Falke, D.; Heicke, B.; Bässler, R

doi:10.1007/bf01241528

Cited by 11 publications

(3 citation statements)

References 22 publications

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“…In ts BTN-1 and ts BN-2 cells, the presence of a mutated host enzyme, normally required for viral DNA synthesis irrespective of the level ofherpes-specified enzyme activities, could completely inhibit viral DNA replication at all MOIs, whereas in ts AF-8 and ts-13 cells different mutated host factors normally used, but not absolutely essential, for viral DNA synthesis might reduce such synthesis to very low levels only in the absence of extensive amounts of herpescoded enzymes. The reported in vitro insensitivity of the HSV-1 DNA polymerase to cytosine arabinoside (triphosphate) inhibition (11), coupled with the well-known in vivo inhibitory effect of this drug on HSV-1 DNA synthesis (3,7), is an observation consistent with such a hypothesis; also of interest is the reported resistance of DNA synthesis in HSV-1-transformed hamster cells to cytosine arabinoside inhibition (16). It should be noted that infection of ts AF-8 cells at the nonpermissive temperature and high MOI (50 PFU/cell) in the presence of cytosine arabinoside (40 ,ug/ml) resulted in a complete inhibition of viral DNA synthesis (Yanagi, unpublished data), suggesting that the viral enzyme was still not acting independently.…”

Section: Discussion Conditional Defect In This Cell Mutant As Notedmentioning

confidence: 65%

Inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants

Yanagi¹,

Talavera²,

Nishimoto³

et al. 1978

J Virol

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Herpes simplex virus type 1 DNA synthesis and infectious progeny production were studied in five different conditional hamster (BHK-21) cell cycle mutants. At the nonpermissive temperature (39.50C), both events were strongly inhibited in four ofthese cell lines. The degree ofinhibition was a reproducible characteristic of each cell mutant and in two cases was dependent upon the multiplicity of infection. Experiments involving shifts to the nonpermissive temperature at least 3 h postinfection at 33.50C suggested that the defects in viral replication were not due to faulty adsorption, penetration, or uncoating, whereas experiments involving shifts of infected cells from the nonpermissive temperature to 33.50C revealed the reversible nature of the inhibition.

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Section: Discussion Conditional Defect In This Cell Mutant As Notedmentioning

confidence: 65%

Inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants

Yanagi¹,

Talavera²,

Nishimoto³

et al. 1978

J Virol

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“…Absorption of serum with cells infected with the heterologous type for MA also affected the neutralization test but a p parently not to the same degree (Table I). These results might be a consequence of the sensitivity of the 2 methods or may indicate that the envelope antigens of HSV virions are not completely equivalent to surface antigens which appear on the plasma membrane of infected cells as suggested by Falke et al (13).…”

Section: Resuzts As Shown Inmentioning

confidence: 85%

Typing of Isolates of Herpes Simplex Virus by Mixed Agglutination

Ito

Barron

1974

Experimental Biology and Medicine

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The characteristics of herpes simplex virus (HSV) type 1 surface antigen(s) appearing on infected cells have been studied in our laboratory by the method of mixed agglutination (MA) (1). Since it has been demonstrated by membrane immunofluorescence (2, 3) that type specificity may be shown for surface antigens, our studies were extended to characterization of HSV type 2 surface antigen and the use of MA for typing HSV isolates. The results of this investigation are the subject of this communication.Materials and Methods. Cell cultures. BIRK, a continuous line derived from rabbit kidney cells, and HEp-2, were used for detection of HSV surface antigen (1). Cells were grown in Eagle's basal medium (BME) in a base of Hank's balanced salt solution supplemented with 10% fetal calf serum, penicillin (200 units/ml) and streptomycin (200 pg/ml) . After virus inoculation, cultures were maintained in BME in a base of Earle's saline (sodium bicarbonate 1.8 mg/ml), 3% fetal calf serum, and antibiotics. BGM, a continuous line derived from African green monkey kidney cells, was used for assay of infectivity (4). Cells were grown in Eagle's minimal essential medium (MEM) in Earle's saline and maintained in MEM with 3% fetal calf serum following virus inoculation.Viruses. Herpes simplex virus (HSV) type 1 strains were: MacIntyre VR# 3 ( 1 ), F and MP (refer Results) supplied by Dr. €3. Roizman, University of Chicago, Chicago, Ill., and H F propagated in this laboratory. Type 2 strains were: MS ( l ) , and 69-375 from the Erie County Virology Laboratory.Isolates of HSV were supplied by the Erie 41 County Virology Laboratory. Laboratory strains were plaque-purified twice in BGM and passaged once in BIRK. Most HSV isolates were employed after 1-3 passages in BIRK or HEp-2.Sera. Rabbit antisera to HSV type 1 (VR#3), #284, and type 2 (MS), #274 have been previously described ( 1).Absorption of sera. Sera were absorbed with BGM cells infected with either type 1 or type 2. Cells were infected with approximately 0.1-0.3 PFU/cell and incubated at 36" for 24-48 hr. Infected cells were collected by scraping, and after washing with phosphate buffered saline (PBS) pH 7.2, a cell pack was obtained by centrifugation at 2,000 rpm for 15 min. One ml of a 1 : 20 dilution of antiserum was mixed with approximately 5 X lo7 packed infected cells in a volume of approximately 0.1 ml and incubated at 36" for 2 hr followed by further incubation overnight at 5". The serum was removed following centrifugation and absorption was repeated a second time. The absorbed serum was heated at 56" for 20 min to inactivate any infectious virus. Mixed agglutination ( M A ) .The method used has been reported in detail elsewhere (1, 5 ) . The attachment of rabbit anti-HSV antibodies to infected cell cultures was revealed by the indicator system. In the present experiments the indicator cell suspension was prepared with sheep erythrocytes (SRBC) + rabbit anti-SRBC serum + sheep anti-rabbit globulin serum, Grand Island Biological Company, Grand Island, N. Y. The sheep anti...

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“…At this concentration, DNA-synthesis is blocked (3). The drug was added at final concentrations of 6 ~g/ ml.…”

Section: Influence O/ C-ara and Cycloheximide On Tk-inductionmentioning

confidence: 99%

Influence of double infections on the induction of thymidine kinase by UV-irradiated herpes simplex virus types 1 and 2 and pseudorabies virus

Dundaroff¹,

Just²,

Falke³

1975

Archives of Virology

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Simultaneous infection of primary rabbit kidney cells with HSV type 1 TK+ and a TK- strain results in a mutual influence of both viruses on the induction of thymidine kinase (TK). TK+ virus has an enhancing and TK- virus a depressing effect on TK induction by a superinfecting TK+ virus. The enzyme induction depends on the ratio of multiplicities of both viruses. The mutual influence on TK induction depends further on the time of addition of the superinfecting virus: the effect of the second virus can still be observed when given 6 hours after primary infection. Identical phenomena can be observed using combinations with HSV type 2 or Pseudorabies viruses. The ability of HSV to induce TK is progressively inactivated with increasing the time of UV-irradiation. The depressing effect of a TK- strain and the stimulating effect of a TK+ strain on superinfecting TK+ strains is UV-sensitive: after 6 minutes of UV-irradiation neither inhibition nor stimulation of TK induction by a superinfecting TK+ strain can be observed. Infection by long-term (20 minutes) UV-irradiated TK+ strains results in a depression of TK induction by a superinfecting TK+ virus. Long-term irradiation of the TK- virus does not show this effect. Cytosine-arabinoside has no effect on the mutual influence of TK induction by TK+ and TK-strains; the phenomenon of mutual depression therefore has to be considered an early process.

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The effect of arabinofuranosyl-cytosine upon the synthesis of herpesvirus hominis Electron microscopic observations in relation to viral DNA-synthesis

Cited by 11 publications

References 22 publications

Inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants

Inhibition of herpes simplex virus type 1 replication in temperature-sensitive cell cycle mutants

Typing of Isolates of Herpes Simplex Virus by Mixed Agglutination

Influence of double infections on the induction of thymidine kinase by UV-irradiated herpes simplex virus types 1 and 2 and pseudorabies virus

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