The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification

Kim, Hye Jin; Lee, Ki Hwan; Park, Sung Baek; Choi, Young Bae; Yang, Jihyun

doi:10.5653/cerm.2015.42.3.94

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…The latter may increase the chances for ice formation during freezing process, which impairs embryo survival rate (Vanderzwalmen et al, 2002). Performing artificial shrinkage of the blastocoel on grade 3 embryos may increase transfer rate as well as pregnancy outcome (Cao et al, 2014;Kim et al, 2015;Darwish and Magdi, 2016;Levi-Setti et al, 2016). It is widely accepted that embryo aneuploidy contributes to pregnancy loss (Brown, 2008;Suzumori & Sugiura-Ogasawara, 2010;Hyde & Schust, 2015).…”

Section: Discussionmentioning

confidence: 99%

Is it worth it to cryopreserve embryos with blastulation delay at day 5?

Montjean

Pauly

Boyer

et al. 2019

Zygote

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SummaryThis is a retrospective study over a 5-year period. In total, 3139 embryos were individually cryopreserved (Cryotop®) and warmed using the Kitazato vitrification/warming kit. They were classified into three categories based on their expansion degree. Transfer, implantation and pregnancy rates were assessed for each embryo category and compared using SPSS (Statistical Package for the Social Sciences) software. In total, 1139 couples enrolled in infertility treatment programme benefitted from embryo vitrification at day 5. After warming, embryos belonging to the three categories showed similar success rates. Although there was a trend towards better outcomes when grade 3 embryos were transferred, the differences did not reach statistical significance: implantation rates (n fetal sac/n embryo transferred) grade 1: 21.9%, grade 2: 22.7% and grade 3: 30.3% (=0.19). Pregnancy rate (n clinical pregnancy/n transfer) (21.9%, 22.7%, 30.3%, respectively; P=0.11). Miscarriage rate was not statistically different in the three categories (14.5%, 20.4%, 20%, respectively, P=0.51). Our overall results show that it is worth vitrifying slow kinetics embryos as they provide a non-negligible chance to give rise to a pregnancy.

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Section: Discussionmentioning

confidence: 99%

Is it worth it to cryopreserve embryos with blastulation delay at day 5?

Montjean

Pauly

Boyer

et al. 2019

Zygote

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“…Assisted hatching was effective in promoting the hatching rate after the cryopreservation of blastocysts [ 28 ]. Artificial shrinkage in mouse blastocysts combined with assisted hatching before cryopreservation was associated with a higher hatched blastocyst formation rate than observed for mechanical AH [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of laser-assisted hatching and exposure time to vitrification solution on mouse embryo development

Kim

Park²,

Yang

et al. 2017

Clin Exp Reprod Med

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ObjectiveThis study was conducted to investigate the efficacy of laser-assisted hatching (LAH) and various vitrification times for embryonic development and blastocyst cell numbers.MethodsFirst, 2-cell and 8-cell embryos were collected by flushing out the oviducts. In the control groups, they were vitrified for 8 or 10 minutes without LAH. The LAH groups underwent quarter laser zona thinning-assisted hatching before vitrification (4, 6, and 8 minutes or 4, 7, and 10 minutes, respectively). After incubation, double-immunofluorescence staining was performed.ResultsThe hatched blastocyst rate 72 hours after the 2-cell embryos were thawed was significantly higher in the 2LAH-ES8 group (33.3%) than in the other groups (p<0.05). In the control-8 group (22.1±4.6), the cell number of the inner cell mass was higher than in the LAH groups (p<0.05). The number of trophectoderm cells was higher in the 2LAH-ES6 group (92.8±8.9) than in the others (p<0.05). The hatched blastocyst rate 48 hours after the 8-cell embryos were thawed was higher in the 8LAH-ES4 group (45.5%) than in the other groups, but not significantly. The inner cell mass cell number was highest in the 8LAH-ES7 group (19.5±5.1, p<0.05). The number of trophectoderm cells was higher in the 8LAH-ES10 group (73.2±12.1) than in the other groups, but without statistical significance.ConclusionWhen LAH was performed, 2-cell embryos with large blastomeres had a lower hatched blastocyst rate when the exposure to vitrification solution was shorter. Conversely, 8-cell embryos with small blastomere had a higher hatched blastocyst rate when the exposure to vitrification solution was shorter.

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“…The large size and multicellular structure of blastocysts pose a great challenge for vitrification and subsequent warming due to the relatively slow penetration of cryoprotectants into blastocysts and insufficient dehydration of the blastocoel. Therefore, processes such as artificial shrinkage and hatching of blastocysts before vitrification enhance the outcome of vitrification, in terms of reduced DNA damage in blastomeres, 19 better survival, quality, 20,21 and higher hatching rate 22 of post-warmed embryos.…”

Section: Introductionmentioning

confidence: 99%

The adverse effects of vitrification on mouse embryo development and metabolic phenotype in offspring

Chen,

Zhou,

Wang

et al. 2023

The FASEB Journal

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Embryo vitrification is a standard procedure in assisted reproductive technology. Previous studies have shown that frozen embryo transfer is associated with an elevated risk of adverse maternal and neonatal outcomes. This study aimed to explore the effects of mouse blastocyst vitrification on the phenotype of vitrified‐warmed blastocysts, their intrauterine and postnatal development, and the long‐term metabolic health of the derived offspring. The vitrified‐warmed blastocysts (IVF + VT group) exhibited reduced mitochondrial activity, increased apoptotic levels, and decreased cell numbers when compared to the fresh blastocysts (IVF group). Implantation rates, live pup rates, and crown‐rump length at E18.5 were not different between the two groups. However, there was a significant decrease in fetal weight and fetal/placental weight ratio in the IVF + VT group. Furthermore, the offspring of the IVF + VT group at an age of 36 weeks had reduced whole energy consumption, impaired glucose and lipid metabolism when compared with the IVF group. Notably, RNA‐seq results unveiled disturbed hepatic gene expression in the offspring from vitrified‐warmed blastocysts. This study revealed the short‐term negative impacts of vitrification on embryo and fetal development and the long‐term influence on glucose and lipid metabolism that persist from the prenatal stage into adulthood in mice.

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The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification

Cited by 7 publications

References 25 publications

Is it worth it to cryopreserve embryos with blastulation delay at day 5?

Is it worth it to cryopreserve embryos with blastulation delay at day 5?

Effects of laser-assisted hatching and exposure time to vitrification solution on mouse embryo development

The adverse effects of vitrification on mouse embryo development and metabolic phenotype in offspring

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