The effect of ascorbic acid on bone cancer cells <i>in vitro</i>

Fernandes, Gabriela; Barone, Andrew W.; Dziak, Rosemary

doi:10.1080/23312025.2017.1288335

Cited by 17 publications

(12 citation statements)

References 30 publications

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“…Considering the single antioxidants tested, hydroxycinnamic acids seem not to have antiproliferative activity on this cell line ( Figure 1), independently from the concentration (1-500 µM) or the length time of the treatment (24 h or 48 h), in agreement also with Martini et al [21]. The same considerations can be made for ascorbic acid, which does not induce any change in HT29 cells viability in the tested concentrations range (Figure 1), according to what observed by Fernandes et al in 2017 on bone cancer cells cell line [22].…”

Section: Discussionsupporting

confidence: 89%

Biological Effect of Different Spinach Extracts in Comparison with the Individual Components of the Phytocomplex

Arru

Mussi

Forti

et al. 2021

Foods

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The Mediterranean-style diet is rich in fruit and vegetables and has a great impact on the prevention of major chronic diseases, such as cardiovascular diseases and cancer. In this work we investigated the ability of spinach extracts obtained by different extraction methods and of the single main components of the phytocomplex, alone or mixed, to modulate proliferation, antioxidant defense, and genotoxicity of HT29 human colorectal cells. Spinach extracts show dose-dependent activity, increasing the level of intracellular endogenous reactive oxygen species (ROS) when tested at higher doses. In the presence of oxidative stress, the activity is related to the oxidizing agent involved (H2O2 or menadione) and by the extraction method. The single components of the phytocomplex, alone or mixed, do not alter the intracellular endogenous level of ROS but again, in the presence of an oxidative insult, the modulation of antioxidant defense depends on the oxidizing agent used. The application of the phytocomplex extracts seem to be more effective than the application of the single phytocomplex components.

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Section: Discussionsupporting

confidence: 89%

Biological Effect of Different Spinach Extracts in Comparison with the Individual Components of the Phytocomplex

Arru

Mussi

Forti

et al. 2021

Foods

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“…Similarly, the clonogenic survival of both cell lines after treatment with 75 pmole/cell (7.5 mM) of P-AscH − was significantly decreased compared to normal canine fibroblasts (Figure 5B). This is similar to human OSA in vitro studies, which showed a dose-dependent decrease in cell growth following exposure to P-AscH − (range of doses up to 1 mM) and a sparing of normal control cells; the most significant growth effects were found at the 1 mM concentration (50, 51). Similar findings have been confirmed for a variety of human tumors (7).…”

Section: Discussionsupporting

confidence: 85%

In vitro Cytotoxicity and Pharmacokinetic Evaluation of Pharmacological Ascorbate in Dogs

et al. 2019

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Background: High-dose, pharmacological ascorbate (P-AscH−) is preferentially cytotoxic to human cancer cells in vitro. Investigations on the efficacy of P-AscH− as an adjuvant treatment for multiple human cancers are on-going, but has yet to be formally investigated in dogs. The primary objectives of this study were to determine the pharmacokinetic (PK) profile of P-AscH− in healthy Beagle dogs and the effects of P-AscH− on canine osteosarcoma cells in vitro.Methods: Eight purpose-bred, healthy, spayed female Beagle dogs, between 20 and 21 months old, and 8–10 kg were administered two doses of P-AscH− (550 or 2,200 mg/kg) via intravenous infusion over 6 h, on separate days. Plasma ascorbate concentrations were measured at 12 time points during and after infusion for PK analysis using nonlinear mixed-effects (NLME) and non-compartmental analysis (NCA). Clonogenic assays were performed on 2 canine osteosarcoma cell lines (D-17 and OSCA-8) and canine primary dermal fibroblasts after exposure to high concentrations of ascorbate (75 pmoles/cell).Results: Plasma ascorbate levels in the dogs peaked at approximately 10 mM following 2,200 mg/kg and returned to baseline 6–8 h after dosing. Minor adverse effects were seen in two dogs. Ascorbate (75 pmoles/cell) significantly decreased survival in both the osteosarcoma cell lines (D-17 63% SD 0.010, P = 0.005; OSCA-8 50% SD 0.086, P = 0.026), compared to normal fibroblasts (27% SD 0.056).Conclusions: Pharmacological ascorbate is preferentially cytotoxic to canine-derived cancer cells. High levels of ascorbate can be safely administered to dogs. Further studies are needed to determine the effects of P-AscH− on canine patients.

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“…Some methods to reduce the high-failure rate would be to target known signaling pathways [ 22 ], especially pathways that are proven to generate results. By formulating newer drugs to act on these known pathways or targets, it significantly reduces the risk of failure [ 22 , 23 , 24 ]. This transfers the risk to start-up companies that are financed by either bigger pharmaceutical companies or IPOs and is a well-known strategy in other industries that can be applied to drug innovations.…”

Section: Role Of Systems Biology In Drug Discovery and Developmentmentioning

confidence: 99%

The Role of Systems Biologic Approach in Cell Signaling and Drug Development Responses—A Mini Review

2018

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The immune system is an integral aspect of the human defense system and is primarily responsible for and involved in the communication between the immune cells. It also plays an important role in the protection of the organism from foreign invaders. Recent studies in the literature have described its role in the process of hematopoiesis, lymphocyte recruitment, T cell subset differentiation and inflammation. However, the specific molecular mechanisms underlying these observations remain elusive, impeding the elaborate manipulation of cytokine sequential delivery in tissue repair. Previously, the discovery of new drugs and systems biology went hand in hand; although Systems biology as a term has only originated in the last century. Various new chemicals were tested on the human body, and studied through observation. Animal models replaced humans for initial trials, but the interactions, response, dose and effect between animals and humans could not be directly correlated. Therefore, there is a need to form disease models outside of human subjects to check the effectiveness and response of the newer natural or synthetic chemicals. These emulate human disease conditions wherein the behavior of the chemicals would be similar in the disease model and humans.

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The effect of ascorbic acid on bone cancer cells in vitro

Cited by 17 publications

References 30 publications

Biological Effect of Different Spinach Extracts in Comparison with the Individual Components of the Phytocomplex

Biological Effect of Different Spinach Extracts in Comparison with the Individual Components of the Phytocomplex

In vitro Cytotoxicity and Pharmacokinetic Evaluation of Pharmacological Ascorbate in Dogs

The Role of Systems Biologic Approach in Cell Signaling and Drug Development Responses—A Mini Review

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