The effect of aspartame metabolites on human erythrocyte membrane acetylcholinesterase activity

Levels of two oxidative stress indicators of human sickle erythrocytes incubated in aqueous extracts of Anacardium occidentale, Psidium guajava and Terminalia catappa.

“…Serial dilutions of the aqueous extracts in the order of 200, 400, 600 and 800 mg% (w/v) were used for analyses. The erythrocytes were washed by centrifugation methods as described by Tsakiris et al [50] Within 2 h of collection of blood samples, 2.0 ml aliquots of the samples were introduced into centrifuge test tubes containing 4.0 ml of buffer solution pH = 7.4: 250 mM tris (hydroxyl methyl) amino ethane-HCl (Tris-HCl) 140 mM NaCl 1.0 mM MgCl 2 10 mM glucose. The erythrocytes were separated from plasma by centrifugation at 1200 x g for 10 min and washed three times by the same centrifugation method with the buffer solution.…”

Activities of Three Erythrocyte Enzymes of Hyperglycemic Rats (Rattus norvegicus) Treated with Allium sativa Extract.

“…The harvested erythrocytes were washed by previously described methods. [25,26] Within 2 h of collection of the blood specimen, 1.0 mL of harvested erythrocyte was introduced into centrifuge test tubes containing 3.0 mL of buffer solution pH = 7.4: 250 mM tris (hydroxyl methyl) amino ethaneHCl (Tris-HCl)/140 mM NaCl/1.0 mM MgCl 2 /10 mM glucose). The erythrocytes suspension was further centrifuged at 1200 g for 10 min and repeated 3 times to remove platelets and leucocytes.…”

Assessment of air quality and erythrocyte oxidative stress indicators of storekeepers of laboratory/industrial chemicals in owerri, Imo state, Nigeria

“…The erythrocytes were washed by methods described by Tsakiris et al, [3] . Platelets and leucocytes were eliminated from the pelleted erythrocytes by the methods of Kalra et al, [4] .…”