The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide

Kang, Ping; Liu, Yulan; Zhu, Huiling; Li, Shuang; Shi, Haifeng; Chen, Feng; Leng, Weibo; Pi, Dinan; Hou, Yongqing

doi:10.1007/s00394-014-0739-3

Cited by 24 publications

(29 citation statements)

References 38 publications

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“…Indeed, LPS inhibition of AMPK expression in vivo is not limited to adult tissue as a very recent paper described the same phenomenon in weaned piglets (Kang et al . ) and we ourselves find a rapid decrease in AMPKα1 and AMPKα2 mRNA expression in response to LPS in neonatal rats (Hagberg H, unpublished data).…”

Section: Discussionmentioning

confidence: 76%

A dual role for AMP‐activated protein kinase (AMPK) during neonatal hypoxic–ischaemic brain injury in mice

Rousset

Leiper

Kichev

et al. 2015

Journal of Neurochemistry

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Perinatal hypoxic-ischaemic encephalopathy (HIE) occurs in 1-2 in every 1000 term infants and the devastating consequences range from cerebral palsy, epilepsy and neurological deficit to death. Cellular damage post insult occurs after a delay and is mediated by a secondary neural energy failure. AMP-activated protein kinase (AMPK) is a sensor of cellular stress resulting from ATP depletion and/or calcium dysregulation, hallmarks of the neuronal cell death observed after HIE. AMPK activation has been implicated in the models of adult ischaemic injury but, as yet, there have been no studies defining its role in neonatal asphyxia. Here, we find that in an in vivo model of neonatal hypoxia-ischaemic and in oxygen/glucose deprivation in neurons, there is pathological activation of the calcium/calmodulin-dependent protein kinase kinase b (CaMKKb)-AMPKa1 signalling pathway. Pharmacological inhibition of AMPK during the insult promotes neuronal survival but, conversely, inhibiting AMPK activity prior to the insult sensitizes neurons, exacerbating cell death. Our data have pathological relevance for neonatal HIE as prior sensitization such as exposure to bacterial infection (reported to reduce AMPK activity) produces a significant increase in injury.

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Section: Discussionmentioning

confidence: 76%

A dual role for AMP‐activated protein kinase (AMPK) during neonatal hypoxic–ischaemic brain injury in mice

Rousset

Leiper

Kichev

et al. 2015

Journal of Neurochemistry

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“…Additionally, Wu et al (2014b) have demonstrated that dietary Glu can protect piglets from deoxynivalenol exposure by increasing the host's anti-oxidative capability and protein synthesis through activating the mammalian target of rapamycin (mTOR) signaling pathway. There is also evidence that Asp alleviates intestinal barrier damage and dysfunctional liver energy metabolism in piglets challenged with LPS (Kang et al 2015;Pi et al 2014). Thus, the beneficial contribution of Glu and Asp on alleviating oxidative stress may be ascribed to their regulatory function in anti-oxidative reactions, protein synthesis, and metabolism.…”

Section: Discussionmentioning

confidence: 96%

“…The content of Glu and Asp and other amino acids in diets were measured (Table 2), and l-alanine (Ala) (purity >99 %, Beijing Chemclin Biotech, Beijing, China) was used as the isonitrogenous control, as previously described (Hu et al 2015;Kim and Wu 2004;Sun et al 2015). The dosage of H 2 O 2 used in this study was based on our previous study (Yin et al 2015a, b) and the dosages of Glu and Asp supplementation were based on previous works (Kang et al 2015;Yin et al 2015a, b). This study was approved by Laboratory Animal welfare Commission of the Institute of Subtropical Agriculture, Chinese Academy of Sciences.…”

Section: Experimental Designmentioning

confidence: 99%

“…Furthermore, Glu improves animal growth performance, regulate the metabolism of lipid and nitrogen to favor reduction in the whole-body white fat and growth of lean tissue (Boutry et al 2012;Chen et al 2014), beneficially modulates the gastrointestinal absorptive system (Zhang et al 2013), and enhances host anti-oxidative ability , and protects animals from damage caused by toxins exposure (Duan et al 2014;Wu et al 2014b). Asp has been reported to restore intestinal barrier and improve liver metabolism in piglets challenged with lipopolysaccharide (LPS) (Kang et al 2015;Pi et al 2014) possibly because of its contributions for the tricarboxylic acid cycle and the production of ATP (Russell and Taegtmeyer 1991). Considering their important versatile functions in physiology and nutrition, Glu and Asp are able to protect animals and human from oxidative stress.…”

Section: Introductionmentioning

confidence: 98%

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Dietary supplementation with l-glutamate and l-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide

et al. 2015

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This study was to evaluate the protective roles of L-glutamate (Glu) and L-aspartate (Asp) in weaned piglets challenged with H2O2. Forty weaned piglets were assigned randomly into one of five groups (8 piglets/group): (1) control group (NC) in which pigs were fed a corn- and soybean meal-based diet and received intraperitoneal administration of saline; (2) H2O2 group (PC) in which pigs were fed the basal diet and received intraperitoneal administration of 10 % H2O2 (1 ml/kg body weight once on days 8 and repeated on day 11); (3) PC + Glu group (PG) in which pigs were fed the basal diet supplemented with 2.0 % Glu before intraperitoneal administration of 10 % H2O2; (4) PC + Asp group (PA) in which pigs were fed the basal diet supplemented with 1.0 % Asp before intraperitoneal administration of 10 % H2O2; (5) PC + Glu + Asp group (PGA) in which pigs were fed the basal diet supplemented with 2.0 % Glu plus 1.0 % Asp before intraperitoneal administration of 10 % H2O2. Measured parameters included daily feed intake (DFI), average daily gain (ADG), feed conversion rate (FCR), and serum anti-oxidative enzyme activities (catalase, superoxide dismutase, glutathione peroxidase-1), serum malondialdehyde and H2O2 concentrations, serum amino acid (AA) profiles, and intestinal expression of AA transporters. Dietary supplementation with Glu, Asp or their combination attenuated the decreases in DFI, ADG and feed efficiency, the increase in oxidative stress, the alterations of serum AA concentrations, and the changed expression of intestinal AA transporters in H2O2-challenged piglets. Thus, dietary supplementation with Glu or Asp alleviates growth suppression and oxidative stress, while restoring serum the amino acid pool in H2O2-challenged piglets.

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“…It is not only an important flavor amino acid but also an important osmotic regulator, playing an important role in regulating the osmotic pressure and ionic composition in fish (Bender, 2012). The content of Asp was the second highest amino acids, which is also a flavor amino acid, and plays an important role in mitochondrial energy metabolism, nitrogen metabolism, the production of neurotransmitter in central nervous system and urea cycle (Kang et al, 2015). Tau was detected in all these 3 groups, which is involved in the maintenance and regulation of almost all normal physiological activities in organisms (Lambert et al, 2015).…”

Section: Fatty Acid Profilementioning

confidence: 99%

Comparative study of nutritional composition of adults and juveniles of Coilia mystus collected from the Yangtze Estuary

Song

Zhao

Feng

et al. 2018

IJAR

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Nutritional composition of the adults (female and male) and juveniles of Coilia mystus were investigated using the national standard methods. The results showed that: (1) Adult females has the highest content of fat, while the juveniles has the highest content of protein and moisture. (2) The juveniles has the highest content of Lys, Glu, Asp, TAA, SEAA, NEAA, FAA, while the adult females have the lowest content. (3) The juveniles have the highest content of DHA, EPA, DPA, ARA, PUFA, n3PUFA, n6PUFA, while the adult males have the lowest content. The above analysis showed that the juveniles took diet more balanced in essential amino acid and abundant in essential fatty acid than that taken by adults. The juveniles showed better edible value and health function than the adults, which is more suitable for development and utilization as a natural food by the whole.

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The effect of aspartate on the energy metabolism in the liver of weanling pigs challenged with lipopolysaccharide

Abstract: These results indicated that dietary supplementation of Asp could improve energy status in LPS-injured liver, which might result from motivating the metabolism pathway of TCA cycle and glycolysis and stimulating the AMPK signaling pathway.

Cited by 24 publications

References 38 publications

A dual role for AMP‐activated protein kinase (AMPK) during neonatal hypoxic–ischaemic brain injury in mice

A dual role for AMP‐activated protein kinase (AMPK) during neonatal hypoxic–ischaemic brain injury in mice

Dietary supplementation with l-glutamate and l-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide

Comparative study of nutritional composition of adults and juveniles of Coilia mystus collected from the Yangtze Estuary

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