The Effect of Autologous Platelet-Rich Plasma on Bronchial Stump Tissue Granulation after Pneumonectomy: Experimental Study

Abstract

Objectives. Recent advances in perioperative management, antibiotics, and surgical materials, including mechanical staplers, have decreased the operative risk of pulmonary resection. However, bronchopleural fistula can still occur in some instances, the occurrence often being lethal. This study investigated whether platelet-rich plasma (PRP) promotes granulation of the bronchial stump after pneumonectomy. Methods. Ten pigs were randomized into two groups: (A) control or non-PRP group (pneumonectomy) and (B) PRP group (pneumonectomy and PRP application). PRP was obtained by spinning down the animal's own blood and collecting the buffy coat containing platelets and white blood cells. Results. Increased platelet concentration triggered the healing process. The percentage of granulation tissue formed at the stumps was significantly higher in the PRP group of animals. This observation was confirmed when statistical analysis using Mann-Whitney U test was performed (P = 0.0268). Conclusions. PRP is easily produced with minimal basic equipment and is useful in accelerating granulation of the bronchial stump, although the timing and optimum number of applications in humans require further study. Autologous PRP is a safe, feasible, and reliable new healing promoter with potential therapeutic effects.

Efficiency of platelet rich plasma in tracheotomy

“…Spartalis ve ark. [19] domuzlar ile yaptıkları bir hayvan deneyinde, pnömonektomi sonrası bronş güdüğünün iyileşmesinde TZP'nin etkinliğini araştırmışlar ve yaptıkları sol pnömonektomi sonrası bir grubun bronş güdüğü üzerine TZP uygulamışlar, TZP uygulanan grubun bronş güdüğünde granülasyonun deney sonrası hızlandığını tespit etmişlerdir. Bizim çalışmamızda da TZP uygulanan trakealarda mikroskopik olarak granülasyon dokusunun iyi geliştiğini tespit ettik.…”

The “Yin and Yang” of Platelet-rich Plasma in Breast Reconstruction After Mastectomy or Lumpectomy for Breast Cancer

“…The principal debate around PRP use in cancer surgery pertains to whether it is oncologically safe to apply PRP to sites where cancer has been earlier developed and excised (44)(45)(46)(47)(48)(49)(50)(51). To date, data on the use of PRP as an adjunct to breast reconstruction after cancer treatment remain limited as opposed to adipose-derived stem cells (ASCs) or autologous fat grafting (38).…”

Use of Platelet Rich Plasma (PRP) in the Treatment of Acute Post Intubation Tracheal Rupture: Report of Two Cases

“…We have found only two references of experimental work with PRP on airway lesions. Gomez-Caro et al in an experimental pig model of tracheal resection and end to end anastomosis with local PRP application show that this substance accelerates the onset of healing by promoting an earlier release of platelet derived growth factors that stimulate transanastomotic angiogenesis thus reducing complications of surgery [27] and Spartalis et al obtain accelerated granulation of the bronchial stump after pneumonectomy in pigs by locally applying a clot of PRP covering the bronchial suture line [28].…”