The effect of bacterial contamination of semen on sperm chromatin integrity and standard semen parameters in men from infertile couples

Rybar, R.; Prinosilova, P.; Kopecka, Vera; Hlavicova, J.; Vĕzník, Z; Zajicova, A.; Rubeš, Jiřı́

doi:10.1111/j.1439-0272.2011.01198.x

Cited by 54 publications

(43 citation statements)

References 60 publications

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“…However, Rybar et al concluded that the contaminated semen with C. trachomatis, Ureaplasma and Mycoplasma spp. were not associated with sperm DNA fragmentation (85).…”

Section: The Effects Of Bacterial Infection On Sperm Chromatin Condenmentioning

confidence: 96%

“…The effect of the male genital tract infection depends on the pathogen type, acute or chronic condition as well as the site of infection, where the inflammation can occur in the epididymis, prostate gland or seminal vesicles (66). In certain situation the genital tract inflammation become difficult to diagnose because the symptoms may not be apparent (Asymptomatic infection) and the patients only suffering from some local discomfort (85).…”

Section: The Effects Of Bacterial Infection On Sperm Chromatin Condenmentioning

confidence: 99%

See 1 more Smart Citation

The Impact of Bacterial Infections on Human Spermatozoa

Zeyad¹,

Amor²,

Hammadeh³

2017

International Journal of Women’s Health and Reproduction Scienc

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“…However, Rybar et al concluded that the contaminated semen with C. trachomatis, Ureaplasma and Mycoplasma spp. were not associated with sperm DNA fragmentation (85).…”

Section: The Effects Of Bacterial Infection On Sperm Chromatin Condenmentioning

confidence: 96%

Section: The Effects Of Bacterial Infection On Sperm Chromatin Condenmentioning

confidence: 99%

The Impact of Bacterial Infections on Human Spermatozoa

Zeyad¹,

Amor²,

Hammadeh³

2017

International Journal of Women’s Health and Reproduction Scienc

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“…11 Certain types of bacteria, including Mycoplasma, Ureaplasma, Escherichia coli, and Chlamydia trachomatis, may be more harmful to sperm function than others. 11,12 Resistance patterns of bacteria in semen samples have not been frequently reported. In one study by Isaiah et al, semen samples of 140 men in Nigeria were examined, and 59% of the bacteria cultured were resistant to oxacillin.…”

Section: Discussionmentioning

confidence: 99%

Time trends for bacterial species and resistance patterns in semen in patients undergoing evaluation for male infertility

Machen

Bird

Brown

et al. 2018

Baylor University Medical Center Proceedings

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Semen from asymptomatic men who are being evaluated as male partners in interfile couples have been reported to contain a variety of bacteria. Longitudinal studies of the variation of these bacteria over time and their resistance patterns have not been commonly reported. At our institution, residues from semen samples are routinely evaluated for bacteria, including antibiotic sensitivity profiles. We set out to profile the changes in semen bacteria and antibiotic resistance at our institution over time. A total of 72 semen isolates were examined for type of bacteria and sensitivity to a panel of antibiotics. The results were divided into two separate 5-year intervals (the first beginning in 2006, the second in 2011) and compared. The majority of bacteria were skin flora, with Streptococcus and Staphylococcus being the most prevalent. The resistance data for these two pathogens showed minimal statistically significant difference between the two time periods, although the Staphylococcus species did show a trend toward increasing resistance, suggesting that antibiotics currently used in sperm cell preparations may need to be varied.KEYWORDS Bacterial resistance; infertility; male; semen; semen analysis S emen from asymptomatic men being evaluated for infertility has been reported to contain a variety of bacteria. Frequently, these results are from contamination, the most likely sources of which are the male urethra or skin secondary to improper collection or colonization.1,2 Further, incidentally identified semen bacteria in the absence of symptoms are typically considered to have limited, if any, clinical significance.1,3 These species vary by population, but the most common bacteria are typically gram-positive organisms, such as Staphylococcus or Streptococcus species. 4 Reports of semen samples positive for bacteria are extremely variable, with rates of 45% to 100%. 5Longitudinal studies of the variation of these bacteria over time as well as their resistance patterns have not been commonly reported. At our institution, to ensure appropriate processing of semen, residues from semen samples are routinely evaluated for bacteria with antibiotic sensitivity profiles. We set out to profile the changes in semen bacteria and antibiotic resistance at our institution over time. METHODSAfter institutional review board approval was obtained, a retrospective review was undertaken over a 10-year period from 2006 to 2016. Approximately every 2 months, the residues of semen samples collected were sent for microbiology evaluation. The samples were typically leftover portions from freshly collected specimens of men undergoing fertility evaluations. Men were advised to abstain from intercourse 3 to 5 days prior to collection, wash their hands and genitals thoroughly before sample production, and produce the sample into a sterile specimen cup. Isolates were then examined for sensitivity to a panel of antibiotics. The results were divided into two separate 5-year intervals (the first beginning in 2006, the second in 2011) a...

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“…Unfortunately, despite successful antibiotic therapy, semen quality parameters did not improve at least up to 3 months after the therapy. Sperm chromatin integrity assessed by the presence of DNA breaks was not seen with these infections [72]. Also, when looking at the prevalence of these infections, there was no difference comparing fertile to infertile couples [73].…”

Section: Urethritismentioning

confidence: 96%

“…It has been shown that the presence of elementary bodies of C. trachomatis serovar E within the lab setting caused a significant decrease in motile sperm with an increase of dead spermatozoa [60]. The detection of Chlamydia in either semen or urine has been associated with a reduction in sperm numbers [61], reduction of the percentage of progressively motile sperm [62][63][64][65], and abnormal morphology and viability [65].…”

Section: Chlamydia and Fertilitymentioning

confidence: 98%