The effect of bacterial glutathione S‐transferase on morpholine degradation

Emtiazi, Giti; Saleh, Tayebe; Hassanshahian, Mehdi

doi:10.1002/biot.200800238

Cited by 32 publications

(14 citation statements)

References 13 publications

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“…No dichloromethane dehalogenase activity was seen for both isoforms, implying that they were not of the theta class GST. Inference had been made on the ability of GSTs to degrade morpholine [33]. In this study, the ability of both isoforms to conjugate pesticides was also investigated.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Affinity-Purified Isoforms ofAcinetobacter calcoaceticusY1 Glutathione Transferases

Chee

Tan

Alias

2014

The Scientific World Journal

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Glutathione transferases (GST) were purified from locally isolated bacteria, Acinetobacter calcoaceticus Y1, by glutathione-affinity chromatography and anion exchange, and their substrate specificities were investigated. SDS-polyacrylamide gel electrophoresis revealed that the purified GST resolved into a single band with a molecular weight (MW) of 23 kDa. 2-dimensional (2-D) gel electrophoresis showed the presence of two isoforms, GST1 (pI 4.5) and GST2 (pI 6.2) with identical MW. GST1 was reactive towards ethacrynic acid, hydrogen peroxide, 1-chloro-2,4-dinitrobenzene, and trans,trans-hepta-2,4-dienal while GST2 was active towards all substrates except hydrogen peroxide. This demonstrated that GST1 possessed peroxidase activity which was absent in GST2. This study also showed that only GST2 was able to conjugate GSH to isoproturon, a herbicide. GST1 and GST2 were suggested to be similar to F0KLY9 (putative glutathione S-transferase) and F0KKB0 (glutathione S-transferase III) of Acinetobacter calcoaceticus strain PHEA-2, respectively.

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Section: Resultsmentioning

confidence: 99%

Characterization of Affinity-Purified Isoforms ofAcinetobacter calcoaceticusY1 Glutathione Transferases

Chee

Tan

Alias

2014

The Scientific World Journal

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“…Another interestingly gene cluster was PA2811-PA2813 encoding two ABC transporter and a glutathione S-transferase (GST). GSTs constitute a large family of enzymes that catalyze the addition of glutathione to many toxic exogenous compounds [46]. The 9 genes that were over-expressed included oprD , nosFY (PA3394-95 nitrous oxide reductases), hpcB (PA4124, xenobiotic reductase), hpaA (PA4091, 4-hydroxyphenylacetate 3-monooxygenase large chain), PA3951 (molybdopterin biosynthetic protein B1), narK1 (PA3877, nitrite extrusion protein 1) and PA1875 (probable outer membrane protein).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling reveals links between ParS/ParR, MexEF-OprN, and quorum sensing in the regulation of adaptation and virulence in Pseudomonas aeruginosa

et al. 2013

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BackgroundThe ParS/ParR two component regulatory system plays critical roles for multidrug resistance in Pseudomonas aeruginosa. It was demonstrated that in the presence of antimicrobials, ParR enhances bacterial survival by distinct mechanisms including activation of the mexXY efflux genes, enhancement of lipopolysaccharide modification through the arn operon, and reduction of the expression of oprD porin.ResultsIn this study, we report on transcriptomic analyses of P. aeruginosa PAO1 wild type and parS and parR mutants growing in a defined minimal medium. Our transcriptomic analysis provides the first estimates of transcript abundance for the 5570 coding genes in P. aeruginosa PAO1. Comparative transcriptomics of P. aeruginosa PAO1 and par mutants identified a total of 464 genes regulated by ParS and ParR. Results also showed that mutations in the parS/parR system abolished expression of the mexEF-oprN operon by down-regulating the regulatory gene mexS. In addition to the known effects on drug resistance genes, transcript abundances of the quorum sensing genes (rhlIR and pqsABCDE-phnAB) were higher in both parS and parR mutants. In accordance with these results, a significant portion of the ParS/ParR regulated genes belonged to the MexEF-OprN and quorum sensing regulons. Deletion of the par genes also led to increased phenazine production and swarming motility, consistent with the up-regulation of the phenazine and rhamnolipid biosynthetic genes, respectively.ConclusionOur results link the ParS/ParR two component signal transduction system to MexEF-OprN and quorum sensing systems in P. aeruginosa. These results expand our understanding of the roles of the ParS/ParR system in the regulation of gene expression in P. aeruginosa, especially in the absence of antimicrobials.

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“…Oil pollution may arise either accidentally or operationally whenever oil is produced, transported, stored and processed or used at sea or on land. Oil spills are a major menace to the environment as they severely damage the surrounding ecosystems (Head et al, 2006;Emtiazi et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Aliphatic Hydrocarbons by Bacteria Isolated from Khor Al-Zubair Channel, Southern Iraq

Al-Taee¹,

Alkanany²,

Gmais³

et al. 2017

ijms

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Four bacterial strains capable of using crude oil as the sole carbon source were isolated from Khor Al-Zubair channel, southern Iraq. The isolates were identified as Vibrio vulnificus; Brevundimonas diminuta/vesicularis; Ochrobactrum anthropic and Sphingomonas paucimobilis by the Vitek II system. The bacteria grew aerobically in liquid culture containing four concentrations (0.25%, 0.5%, 1% and 2% v/v) of crude oil at 30ᵒC for a seven day period. The components of crude oil degraded by the isFolates were determined by capillary gas chromatography. Vibrio vulnificus was the most effective bacteria to degrade crude oil, especially in concentration 2% (93.05%). Meanwhile, Ochrobactrum anthropi was the lowest (9.63%) in concentration 0.25%.

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The effect of bacterial glutathione S‐transferase on morpholine degradation

Cited by 32 publications

References 13 publications

Characterization of Affinity-Purified Isoforms ofAcinetobacter calcoaceticusY1 Glutathione Transferases

Characterization of Affinity-Purified Isoforms ofAcinetobacter calcoaceticusY1 Glutathione Transferases

Transcriptome profiling reveals links between ParS/ParR, MexEF-OprN, and quorum sensing in the regulation of adaptation and virulence in Pseudomonas aeruginosa

Biodegradation of Aliphatic Hydrocarbons by Bacteria Isolated from Khor Al-Zubair Channel, Southern Iraq

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