The Effect of Bifidobacterium Breve and Lactobacillus Salivarius on the Isoepoxydon Dehydrogenase (Idh) Gene Expression Involved in Patulin Biosynthesis Produced by Penicillium Expansum

Abstract

Penicillium expansum produced the toxin patulin in pome fruits. To evaluate the molecular mechanism by which the treatment of probiotic bacteria Bifidobacterium breve and Lactobacillus salivarius could modulate patulin production, isoepoxydon dehydrogenase (idh) gene involved in the biosynthesis of patulin were measured using real time-PCR technique. The result of this study found that the supplementation with B. breve and L. salivarius down-regulate the relative expression of isoepoxydon dehydrogenase (idh) gene .However, these finding suggested that these bacteria decreased patulin production through effect on the genes responsible for its biosynthesis which can be the main mechanism by which patulin reduced in the presence of the these bacteria.