The effect of bioactive glasses on bone marrow stromal cells differentiation

Bosetti, Michela; Cannas, M.

doi:10.1016/j.biomaterials.2004.09.059

Cited by 205 publications

(152 citation statements)

References 27 publications

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“…These findings are in agreement with previous studies demonstrated that BG has been beneficial to repair periodontal osseous lesions (Nevins et al, 2000) and intra-bony defects (Satyanarayana et al, 2012). These findings could be attributed to BG stimulates bone marrow mesenchymal stem cells to differentiate into osteoblast-like cells with a higher mineralized tissue formation (Bosetti and Cannas, 2005). Furthermore, herein, there was no significant statistical difference between silicate BG and PRP/BG in bone healing after 60 days.…”

Section: Discussionsupporting

confidence: 93%

Novel Practical Insight into Application of Platelet-Rich Plasma in Combination with Bioresorbable Silicate 45S5 Bioactive Glass for Bone Defect Repair: an Experimental Study in a Rabbit Model

Samy¹

2014

Adv. Anim. Vet. Sci.

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| This study was aimed to verify the individual or combining effect of platelet-rich plasma (PRP) and unique biodegradable silicate 45S5 bioactive glass (BG) scaffold on regeneration of metaphyseal tibia defect in a rabbit model. Thirty-six animals were allocated into three groups (n=12 each). In each group, during surgery, right-sided defects were filled with BG, PRP or BG/PRP (1:1), while left-sided defects were served as a control. To visualize and trace defects healing, macroscopic, radiologic and histological examinations were performed at different time points. Bone density/radiopacity and macroscopic (defect size) values were collected and analyzed statistically. Histomorphometric quantitative analysis of H&E stained sections to quantify the percent bone formation at 6 and 8 weeks after implantation was also performed. The gained results showed a significant increase of bone regeneration by 60 days in both BG and BG/PRP-groups (P<0.05 and P<0.001, respectively). Whereas, the healing pattern created by PRP/BG was better comparing to that of BG alone. We noticed unexpectedly incomplete defect closure in PRP-group by 60 days, even though and interestingly, PRP alone accelerated bone radiopacity and new osseous formation significantly only at early stages of healing period (P<0.01). Histomorphometric analyzed data indicated an increasing bone formation over time in all treated defects except PRP during 8 weeks. In conclusion, PRP alone does not provide an appreciated healing effect; however, in future, use of PRP combined with 45S5 BG might be clinically beneficial for proper bone regeneration as safe and appropriate biomaterial.

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Section: Discussionsupporting

confidence: 93%

Novel Practical Insight into Application of Platelet-Rich Plasma in Combination with Bioresorbable Silicate 45S5 Bioactive Glass for Bone Defect Repair: an Experimental Study in a Rabbit Model

Samy¹

2014

Adv. Anim. Vet. Sci.

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“…BG 13-93 is biodegradable, and when placed in an aqueous environment at 37°C, it converts to a calcium phosphate material analogous to hydroxyapatite [20,26]. As BG degrades, it also releases its ion content, which has the ability to stimulate osteoblast activity [3,4,40,42]. Previous studies have shown, in an osteoblast cell line, increasing the pH can improve collagen Type I production by increasing cellular metabolism [10,42].…”

Section: Discussionmentioning

confidence: 99%

“…Our work with an alternative silicate-based glass composition, Bioactive Glass 13-93 (BG 13-93), shows this composition can be readily shaped into porous constructs of complex shapes, while retaining the desirable in vitro bioactive characteristics of 45S5 glass and higher mechanical strength [16,32]. It is known, as BG degrades, it also releases its ion content, which has the ability to stimulate osteoblast activity [3,4,40,42]. However, there is currently a lack of studies investigating the effect of BG 13-93 on chondrocyte behavior and its use in cartilage tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Glass 13-93 as a Subchondral Substrate for Tissue-engineered Osteochondral Constructs: A Pilot Study

Jayabalan

Tan

Rahaman

et al. 2011

Clinical Orthopaedics &Amp; Related Research

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Background Replacement of diseased areas of the joint with tissue-engineered osteochondral grafts has shown potential in the treatment of osteoarthritis. Bioactive glasses are candidates for the osseous analog of these grafts. Questions/purposes (1) Does Bioactive Glass 13-93 (BG 13-93) as a subchondral substrate improve collagen and glycosaminoglycan production in a tissue-engineered cartilage layer? (2) Does BG 13-93 as a culture medium supplement increase the collagen and glycosaminoglycan production and improve the mechanical properties in a tissue-engineered cartilage layer?Methods In Study 1, bioactive glass samples (n = 4) were attached to a chondrocyte-seeded agarose layer to form an osteochondral construct, cultured for 6 weeks, and compared to controls. In Study 2, bioactive glass samples (n = 5) were cocultured with cell-seeded agarose for 6 weeks. The cell-seeded agarose layer was exposed to BG 13-93 either continuously or for the first or last 2 weeks in culture or had no exposure. Results Osteochondral constructs with a BG 13-93 base had improved glycosaminoglycan deposition but less collagen II content. Agarose scaffolds that had a temporal exposure to BG 13-93 within the culture medium had improved mechanical and biochemical properties compared to continuous or no exposure. Conclusions When used as a subchondral substrate, BG 13-93 did not improve biochemical properties compared to controls. However, as a culture medium supplement, BG 13-93 improved the biochemical and mechanical properties of a tissue-engineered cartilage layer. Clinical Relevance BG 13-93 may not be suitable in osteochondral constructs but could have potential as a medium supplement for neocartilage formation.

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“…30 To represent the osteogenic progression, immunohistochemistry was performed to detect the expression of BMP-2, which is secreted at the beginning of osteoid matrix deposition. 31 The immunohistochemical staining results showed that the BMP-2 staining intensity for the MWC scaffolds increased with time and exhibited obviously high intensity as compared with WP scaffolds at 12 weeks. Furthermore, the positive expression ratio of BMP-2 for the MWC scaffolds was significantly higher than WP scaffolds at 8 and 12 weeks.…”

mentioning

confidence: 91%

Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration

Xia¹,

Zhou²,

Wang³

et al. 2016

IJN

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In this study, bioactive scaffold of nano magnesium phosphate (nMP)/wheat protein (WP) composite (MWC) was fabricated. The results revealed that the MWC scaffolds had interconnected not only macropores (sized 400–600 μm) but also micropores (sized 10–20 μm) on the walls of macropores. The MWC scaffolds containing 40 w% nMP had an appropriate degradability in phosphate-buffered saline and produced a weak alkaline microenvironment. In cell culture experiments, the results revealed that the MWC scaffolds significantly promoted the MC3T3-E1 cell proliferation, differentiation, and growth into the scaffolds. The results of synchrotron radiation microcomputed tomography and analysis of the histological sections of the in vivo implantation revealed that the MWC scaffolds evidently improved the new bone formation and bone defects repair as compared with WP scaffolds. Moreover, it was found that newly formed bone tissue continued to increase with the gradual reduction of materials residual in the MWC scaffolds. Furthermore, the immunohistochemical analysis further offered the evidence of the stimulatory effects of MWC scaffolds on osteogenic-related cell differentiation and new bone regeneration. The results indicated that MWC scaffolds with good biocompability and degradability could promote osteogenesis in vivo, which would have potential for bone tissue repair.

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The effect of bioactive glasses on bone marrow stromal cells differentiation

Cited by 205 publications

References 27 publications

Novel Practical Insight into Application of Platelet-Rich Plasma in Combination with Bioresorbable Silicate 45S5 Bioactive Glass for Bone Defect Repair: an Experimental Study in a Rabbit Model

Novel Practical Insight into Application of Platelet-Rich Plasma in Combination with Bioresorbable Silicate 45S5 Bioactive Glass for Bone Defect Repair: an Experimental Study in a Rabbit Model

Bioactive Glass 13-93 as a Subchondral Substrate for Tissue-engineered Osteochondral Constructs: A Pilot Study

Degradability, biocompatibility, and osteogenesis of biocomposite scaffolds containing nano magnesium phosphate and wheat protein both in vitro and in vivo for bone regeneration

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