The effect of bioartificial constructs that mimic myocardial structure and biomechanical properties on stem cell commitment towards cardiac lineage

Cristallini, Caterina; Rocchietti, Elisa Cibrario; Accomasso, Lisa; Folino, Anna; Gallina, Clara; Muratori, Luísa; Pagliaro, Pasquale; Rastaldo, Raffaella; Raimondo, Stefania; Saviozzi, Silvia; Sprio, Andrea Elio; Gagliardi, Mariacristina; Barbani, Niccoletta; Giachino, Claudia

doi:10.1016/j.biomaterials.2013.09.058

Cited by 27 publications

(32 citation statements)

References 52 publications

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“…Investigators have engineered PBHV/gelatin scaffolds resembling myocardial structural properties that, following Overall ANOVA P <.05 A B C stem cell culture, can guide initial MSC and nonmyocyte cell lineages towards cardiomyogenesis as shown by expression of sarcomeric proteins and cardiac transcription factors GATA-4, Nkx2.5, and TBX-5 (67). In an interesting study by Haneef et al (136), electrostimulation of MSCseeded collagen matrices significantly increased MSC proliferation and induced expression of cardiac markers troponin I, connexin 43, sarcomeric ␣-actinin, slow myosin, fast myosin and desmin, compared with nonstimulated control matrices.…”

Section: B Implantation Of Biomimetic Scaffolds and Matricesmentioning

confidence: 99%

Rebuilding the Damaged Heart: Mesenchymal Stem Cells, Cell-Based Therapy, and Engineered Heart Tissue

Golpanian

Wolf

Hatzistergos

et al. 2016

Physiological Reviews

274

211

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Mesenchymal stem cells (MSCs) are broadly distributed cells that retain postnatal capacity for self-renewal and multilineage differentiation. MSCs evade immune detection, secrete an array of anti-inflammatory and anti-fibrotic mediators, and very importantly activate resident precursors. These properties form the basis for the strategy of clinical application of cell-based therapeutics for inflammatory and fibrotic conditions. In cardiovascular medicine, administration of autologous or allogeneic MSCs in patients with ischemic and nonischemic cardiomyopathy holds significant promise. Numerous preclinical studies of ischemic and nonischemic cardiomyopathy employing MSC-based therapy have demonstrated that the properties of reducing fibrosis, stimulating angiogenesis, and cardiomyogenesis have led to improvements in the structure and function of remodeled ventricles. Further attempts have been made to augment MSCs' effects through genetic modification and cell preconditioning. Progression of MSC therapy to early clinical trials has supported their role in improving cardiac structure and function, functional capacity, and patient quality of life. Emerging data have supported larger clinical trials that have been either completed or are currently underway. Mechanistically, MSC therapy is thought to benefit the heart by stimulating innate anti-fibrotic and regenerative responses. The mechanisms of action involve paracrine signaling, cell-cell interactions, and fusion with resident cells. Trans-differentiation of MSCs to bona fide cardiomyocytes and coronary vessels is also thought to occur, although at a nonphysiological level. Recently, MSC-based tissue engineering for cardiovascular disease has been examined with quite encouraging results. This review discusses MSCs from their basic biological characteristics to their role as a promising therapeutic strategy for clinical cardiovascular disease.

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Section: B Implantation Of Biomimetic Scaffolds and Matricesmentioning

confidence: 99%

Rebuilding the Damaged Heart: Mesenchymal Stem Cells, Cell-Based Therapy, and Engineered Heart Tissue

Golpanian

Wolf

Hatzistergos

et al. 2016

Physiological Reviews

274

211

View full text Add to dashboard Cite

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“…In a previous paper we demonstrated the capability of scaffolds based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate)/gelatin (PHBHV/gelatin) to induce stem cell commitment to a cardiac phenotype thanks to an optimal synergy between physico-chemical, mechanical, and structural characteristics [33]. …”

Section: Introductionmentioning

confidence: 99%

Micro‐ and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro

Cristallini

Rocchietti

Gagliardi

et al. 2016

Stem Cells International

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The biomaterial scaffold plays a key role in most tissue engineering strategies. Its surface properties, micropatterning, degradation, and mechanical features affect not only the generation of the tissue construct in vitro, but also its in vivo functionality. The area of myocardial tissue engineering still faces significant difficulties and challenges in the design of bioactive scaffolds, which allow composition variation to accommodate divergence in the evolving myocardial structure. Here we aimed at verifying if a microstructured bioartificial scaffold alone can provoke an effect on stem cell behavior. To this purpose, we fabricated microstructured bioartificial polymeric constructs made of PLGA/gelatin mimicking anisotropic structure and mechanical properties of the myocardium. We found that PLGA/gelatin scaffolds promoted adhesion, elongation, ordered disposition, and early myocardial commitment of human mesenchymal stem cells suggesting that these constructs are able to crosstalk with stem cells in a precise and controlled manner. At the same time, the biomaterial degradation kinetics renders the PLGA/gelatin constructs very attractive for myocardial regeneration approaches.

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“…Most of the investigations showcased the expression of proteins typical of the cardiac tissue, and a spatial distribution or alignment of the cells inside the scaffolds. Some research groups were able to show a striation typical of myofibrils (Engelmayr et al, 2008), others the massive expression of cardiac troponins, formation of intercalated disks, and functional gap junctions (Di Felice et al, 2013; Cristallini et al, 2014). Unfortunately, the extent of differentiation shown is very far from the actual maturation required to rebuild the cardiac tissue complexity.…”

Section: Discussionmentioning

confidence: 99%

“…The combination of PHBHV with gelatin resulted in a 3D scaffold resembling the physico-chemical properties, micro-topography, and mechanics of the myocardium (Cristallini et al, 2014). …”

Section: Cardiac Proto-tissues: Trying To Reproduce the Complexity Ofmentioning

confidence: 99%

Cardiac tissue engineering: a reflection after a decade of hurry

et al. 2014

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The effect of bioartificial constructs that mimic myocardial structure and biomechanical properties on stem cell commitment towards cardiac lineage

Cited by 27 publications

References 52 publications

Rebuilding the Damaged Heart: Mesenchymal Stem Cells, Cell-Based Therapy, and Engineered Heart Tissue

Rebuilding the Damaged Heart: Mesenchymal Stem Cells, Cell-Based Therapy, and Engineered Heart Tissue

Micro‐ and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro

Cardiac tissue engineering: a reflection after a decade of hurry

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