The effect of bioresorbable additives and micro-bioobjects on gel formation, stabilization and thermophysical properties

Abstract

The same properties of agarose gels containing neutral bioresorbable additives and living microorganisms which are important for use in additive technologies of bioreactors creation were considered. Data on the kinetics of gel formation from the solution during cooling were obtained by spectroscopic measurement by measuring the shift of the maximum spectrum of light passing through the gel, depending on the temperature. The dynamics of aging was investigated for gels of different concentrations of agarose, bioresorbable additives and living cells. The time dependences of the decrease in the optical transparency of such gels during the aging process, characterizing the changes in their structure, were obtained. Special attention was paid to the effect of liquid evaporation from gels in the process of gel formation and during long-term storage on relaxation processes leading to their spontaneous increase in density. Experiments were performed to determine the dynamics of the temperature fields simultaneously with heat flux measurements during the formation of studied gels with different concentrations. On the basis of the obtained experimental data and previously developed method, the thermophysical coefficients of agarose gels containing an admixture of starch and living yeast cells were calculated.

Pharmaceutical Applications of Biomass Polymers: Review of Current Research and Perspectives

“…Being non-toxic and biocompatible, agarose is commonly employed as a gelling agent in various applications, including chromatography techniques, nucleic acid electrophoresis, cell culture media, tissue culture overlays, and gel plates. Its exceptional properties, including mechanical resilience and reduced gelling temperature, make it suitable for applications like bio-ink, where gelation forms a 3D network of agarose fibers, disintegrating above 85 • C [63][64][65][66].…”

“…Bei non-toxic and biocompatible, agarose is commonly employed as a gelling agent in vario applications, including chromatography techniques, nucleic acid electrophoresis, cell c ture media, tissue culture overlays, and gel plates. Its exceptional properties, includi mechanical resilience and reduced gelling temperature, make it suitable for applicatio like bio-ink, where gelation forms a 3D network of agarose fibers, disintegrating above °C [63][64][65][66]. Beyond their applications in pharmaceutical, cosmetic, and food industries for c loid stabilization, thickening, protein binding, and gelling, carrageenans also influen plant growth stimulation and serve as pathogen resistance generators, offering crop p tection.…”

Thermokinetics and rheology of agarose gel applied to bioprinting technology

“…In order to study the hysteresis effect of agarose gel, the experimental stand used earlier to determine the thermophysical properties [9] was modernized and based on the optical spectroscopy method, with the implementation of measuring the maximum intensity of the light passing through the sample was used. The scheme of the experimental set-up is shown in fig.1.…”