The Effect of Biscuits Made From Pumpkin Seeds Flour on Serum Zinc Levels and Weight in Malnutrition Wistar Rats

Syam, Aminuddin; Burhan, Fitrah Khaerani; Hadju, Veni; Citrakesumasari, C.; Akhmar, Andi Muhammad

doi:10.3889/oamjms.2020.4402

Cited by 4 publications

(4 citation statements)

References 25 publications

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“…However, the two groups have significant differences with p = 0.007. In line with the study on Wistar mice, giving pumpkin seed flour to malnourished Wistar mice can increase their serum zinc levels [36].…”

Section: Serum Zinc Levels For Pregnant Womenmentioning

confidence: 61%

The Effect of Pumpkin Seed Biscuits on Nutritional and Zinc Status: A Randomized Controlled Trial in Pregnant Women

Syakur

Syam²,

Hadju³

et al. 2022

Open Access Maced J Med Sci

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BACKGROUND: In several studies in Indonesia, low birth weight (LBW) is shown as the most dominant risk factor for stunting. Some of the causes of LBW are chronic energy deficiency during pregnancy, anemia during pregnancy, lack of nutrient supply during pregnancy, pregnancy complications, maternal parity, and birth spacing. In this case, women commonly suffer from micronutrient deficiencies including iodine, iron, Vitamin A, zinc, and folate. Related to this, about 80% of pregnant women in the world fulfill normative pregnancy needs due to inadequate zinc intake. One source of micronutrients rich in zinc is pumpkin seeds. The nutritional potential of pumpkin seeds is realized in the form of food products for pregnant women, including amino acids. One example of pumpkin seed fortification has been assessed in biscuit products by trained panelists and consumers, obtaining results that 20% additional pumpkin seed flour to biscuits becomes the best treatment. Meanwhile, in the case of this study, we expect that the pumpkin seed biscuits provided can increase the energy intake in pregnant women because when pumpkin seeds are added, the nutrition contained in the biscuits is also increased, in this case, the nutrition includes the zinc and Fe. AIM: The purpose this research is to figure out how pumpkin seed biscuits affect pregnant women concerning their nutrition status and serum zinc levels. METHODS: The method employed was a double-blind and randomized experimental research involving pre- and post-test control groups. The inclusion criteria of the participants involved were pregnant women aged 20–35 years old who were at their first and second trimesters of pregnancy and parity one to three and singleton pregnancy. Meanwhile, the exclusion criteria of the participants were pregnant women suffering from severe complications. There were 89 pregnant women further gathered in this study, but only 66 of them met the criteria and idled into two groups. In this case, one group received the pumpkin seed biscuit intervention, while the other received placebo biscuits. The intervention was conducted for 90 days where the pregnant women were provided with four biscuits for daily consumption. RESULTS: The nutritional status parameter of pregnant women involved in the current research was assessed, including body weight, mid-upper arm circumference (MUAC), and serum zinc levels. In this case, the average serum zinc level obtained was 48.75 g/dL from 60 pregnant women. Furthermore, a significant increase occurred in nutritional status for each group, marked by body weight gain, and increased MUAC. However, no significant difference emerged between the pumpkin seed biscuit group and the placebo biscuit group with p = 0.914 and p = 0.916, respectively. A significant increase in zinc levels was observed in both groups. In this case, changes in zinc levels between the two groups were significantly different (13.72 ± 1.84 vs. 9.81 ± 11.81, p = 0.007). CONCLUSION: Giving pumpkin seed biscuits contribute to weight gain and increase the MUAC the pregnant women which, in turn, improves their nutritional status and serum zinc.

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Section: Serum Zinc Levels For Pregnant Womenmentioning

confidence: 61%

The Effect of Pumpkin Seed Biscuits on Nutritional and Zinc Status: A Randomized Controlled Trial in Pregnant Women

Syakur

Syam²,

Hadju³

et al. 2022

Open Access Maced J Med Sci

Self Cite

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“…In an experiment that examined the effect of pumpkin seed flour biscuits on serum zinc concentration and body weight in malnourished Wistar rats, the researchers concluded that preparing biscuits from pumpkin seed flour increased weight gain in malnourished Wistar rats but did not affect zinc serum levels (34). Compared to the control group and saline training, pumpkin seed oil significantly influenced the expression of the ZnT5 transfer gene.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, zinc transporters act as zinc sensors and maintain intracellular zinc homeostasis by responding to zinc availability. Extracellular and intracellular zinc concentrations appear to regulate the expression and distribution of ZIPS and ZnTs (34). Therefore, factors such as exercise and zinc supplements largely determine zinc homeostasis and zinc transporters in the human body.…”

Section: Discussionmentioning

confidence: 99%

Zinc Transporters in the Livers of Healthy Male Wistar Rats: An Investigation of the Effects of Aerobic Exercise and Supplementation with Pumpkin Seed and White Pea

Dashti Khavidaki,

Ghanbari-Niaki,

Nasiri

et al. 2023

Zahedan J Res Med Sci

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Objectives: The current study aims to investigate the impacts of aerobic exercise with pumpkin seed oil and white pea extract supplementation on the gene expression of the liver zinc transporters of healthy male Wistar rats. Methods: In this experimental study, 60 male Wistar rats (130 - 150 g, aged 4-6 weeks old) were divided into 6 groups (n = 10/groups) by randomization. These groups include (1) saline control, (2) saline training, (3) pumpkin seed oil control, (4) pumpkin seed oil training, (5) control-pea, and (6) training-pea. The aerobic exercise protocol consisted of running at a velocity of 25 m/min, 5 days a week and for 6 weeks. Supplement groups received 4 ml/kg/day of pumpkin seed oil and 2 g/kg/day of white pea extract through oral gavage. Gene expression levels of ZnT5, ZnT9, and ZIP14 of liver tissue were determined through real-time PCR, and serum levels of Zn were measured using the ICP-EOS technique approximately 48 hours after the final session of the training program. Data were analyzed by one-way ANOVA and Tukey post-hoc tests (P < 0.05). Results: Regarding the results of one-way ANOVA, there were no significant changes in serum Zn levels (P = 0.418) as well as gene expression levels of ZnT5 and ZIP14 among intervention groups (P = 0.586 and 0.677, respectively), but ZnT9 gene expression level was significantly changed among the study groups (P = 0.029). Also, the results of the Tukey test showed that six weeks of aerobic exercise along with pumpkin seed oil supplementation had the greatest effect on ZnT5 gene expression. Conclusions: Findings showed that aerobic training along with pumpkin seed oil supplementation had the greatest impact on the up-regulation of ZnT5 as a Zn transporter to retain the blood Zn levels in healthy trained rats, suggesting that pumpkin seed oil can be an effective ergogenic aid for improving circulatory Zn levels.

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“…Zn supplementation can increase serum Zn levels, weight growth, and linear increase in premature infants, especially early in life [25]. The providing of Zn-enriched food has been shown to increase linear growth, thereby reducing the number of children under five who have growth disorders [26], [27].…”

Section: Effects Of Providing Local Snacks Accompanied By Virtual Nutrition Counseling On Growth Of Stunting Children Under Fivementioning

confidence: 99%

Local Snacks and Virtual Nutrition Counseling Services Increasing Growth of Stunting Children

Nadimin

Dewi

Salam

et al. 2021

Open Access Maced J Med Sci

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BACKGROUND: The conducive factor of stunting is the deficiency in nutrient intake due to the lack of quantity and quality of food consumed by the children. Children’s food consumption is strongly influenced by the mother’s nutritional knowledge and children’s feeding practices. AIM: The aims of this study were to determine the effect of providing local snacks in South Sulawesi with a substitute of snakehead fish flour (Tibus) and virtual nutrition counseling on the growth of stunting children. METHODS: The design of this study used a randomized pretest-posttest control design. The sample was divided in two groups using systematic random sampling. The first group was given local Tibus snacks and nutritional counseling virtually and the second group was only given virtual nutrition counseling. The intervention was carried out every day until 1 month. The sample of this research was mothers who have stunting children under five of age. RESULTS: Nutrition knowledge of mothers in group one increased significantly before and after intervention (63.78 ± 1.68 vs. 73.70 ±1.35 points), but this was not the case in group two. The increase in maternal nutritional knowledge was higher in group one than in group two, although it was not significant (p = 0.91). The comparison of the weight gain of children in group one versus group two was 0.37 ± 0.49 kg versus 0.07 ± 0.39 kg (p = 0.021). The increase in the height of the children in group one before and after was 84.15 ± 7.79 cm versus 85.97 ± 7.95 cm (p = 0.000). The ratio of the increase in the height of the children in group one and group two was 1.82 ± 0.94 cm versus 1.53 ± 0.68 cm (p = 0.402). CONCLUSIONS: Nutrition counseling virtual can increase nutrition knowledge. The providing of local snacks (Tibus) accompanied by virtual nutrition counseling can increase growth in body length and weight of stunting children. The efforts to accelerate prevention stunting in children under five can be done with utilization of local snacks.

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The Effect of Biscuits Made From Pumpkin Seeds Flour on Serum Zinc Levels and Weight in Malnutrition Wistar Rats

Cited by 4 publications

References 25 publications

The Effect of Pumpkin Seed Biscuits on Nutritional and Zinc Status: A Randomized Controlled Trial in Pregnant Women

The Effect of Pumpkin Seed Biscuits on Nutritional and Zinc Status: A Randomized Controlled Trial in Pregnant Women

Zinc Transporters in the Livers of Healthy Male Wistar Rats: An Investigation of the Effects of Aerobic Exercise and Supplementation with Pumpkin Seed and White Pea

Local Snacks and Virtual Nutrition Counseling Services Increasing Growth of Stunting Children

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