The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles

Allouni, Zouhir Ekeland; Gjerdet, Nils Roar; Cimpan, Mihaela Roxana; Høl, Paul Johan

doi:10.2147/ijn.s72726

Cited by 23 publications

(9 citation statements)

References 59 publications

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“…In vivo , it was shown that proteins adsorb on TiO 2 NPs. In some cases, these NPs induced conformational changes in proteins and affected their functions [ 12 , 59 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

Titanium Dioxide Nanoparticles Increase Superoxide Anion Production by Acting on NADPH Oxidase

et al. 2015

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Titanium dioxide (TiO2) anatase nanoparticles (NPs) are metal oxide NPs commercialized for several uses of everyday life. However their toxicity has been poorly investigated. Cellular internalization of NPs has been shown to activate macrophages and neutrophils that contribute to superoxide anion production by the NADPH oxidase complex. Transmission electron micrososcopy images showed that the membrane fractions were close to the NPs while fluorescence indicated an interaction between NPs and cytosolic proteins. Using a cell-free system, we have investigated the influence of TiO2 NPs on the behavior of the NADPH oxidase. In the absence of the classical activator molecules of the enzyme (arachidonic acid) but in the presence of TiO2 NPs, no production of superoxide ions could be detected indicating that TiO2 NPs were unable to activate by themselves the complex. However once the NADPH oxidase was activated (i.e., by arachidonic acid), the rate of superoxide anion production went up to 140% of its value without NPs, this effect being dependent on their concentration. In the presence of TiO2 nanoparticles, the NADPH oxidase produces more superoxide ions, hence induces higher oxidative stress. This hyper-activation and the subsequent increase in ROS production by TiO2 NPs could participate to the oxidative stress development.

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“…In vivo , it was shown that proteins adsorb on TiO 2 NPs. In some cases, these NPs induced conformational changes in proteins and affected their functions [ 12 , 59 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

Titanium Dioxide Nanoparticles Increase Superoxide Anion Production by Acting on NADPH Oxidase

et al. 2015

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“…37 A recent experimental study showed that TiO 2 NPs adsorbed with blood proteins are less likely to be uptaken by cells. 39 Similarly, previous investigation on blood proteins adsorbed on CNTs also showed a reduced cytotoxicity of CNTs. Therefore, blood proteins can help on the clearance of entered NPs and provide an extra barrier for the cellular uptaken.…”

Section: The Human Serum Albumin Protein On the Nano-particlementioning

confidence: 70%

“…For example, the curvature of a NP can affect the protein adsorption, 39 which shows the size effect of NPs in a toxic process. With new force fields for TiO 2 NPs, many intriguing phenomena in experiment could be interpreted from MD simulations.…”

Section: Resultsmentioning

confidence: 99%

Simplified TiO2 force fields for studies of its interaction with biomolecules

Luan

Huynh

Zhou

2015

The Journal of Chemical Physics

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Engineered TiO2 nanoparticles have been routinely applied in nanotechnology, as well as in cosmetics and food industries. Despite active experimental studies intended to clarify TiO2's biological effects, including potential toxicity, the relation between experimentally inferred nanotoxicity and industry standards for safely applying nanoparticles remains somewhat ambiguous with justified concerns. Supplemental to experiments, molecular dynamics simulations have proven to be efficacious in investigating the molecular mechanism of a biological process occurring at nanoscale. In this article, to facilitate the nanotoxicity and nanomedicine research related to this important metal oxide, we provide a simplified force field, based on the original Matsui-Akaogi force field but compatible to the Lennard-Jones potentials normally used in modeling biomolecules, for simulating TiO2 nanoparticles interacting with biomolecules. The force field parameters were tested in simulating the bulk structure of TiO2, TiO2 nanoparticle-water interaction, as well as the adsorption of proteins on the TiO2 nanoparticle. We demonstrate that these simulation results are consistent with experimental data/observations. We expect that simulations will help to better understand the interaction between TiO2 and molecules.

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“…A disadvantage of these techniques is that there is no possibility to distinguish between NMs that are internalized into the cells, extracellularly associated and/or just located between cells or within the extracellular fluid 19. It has been shown that there is a significant difference between uptake measured by means of ICP‐MS and uptake based on the analysis of individual cells 17…”

Section: Label‐free Dosimetry and Imaging Techniques—toward High Thromentioning

confidence: 99%

High throughput toxicity screening and intracellular detection of nanomaterials

Collins

Annangi

Rubio

et al. 2016

WIREs Nanomed Nanobiotechnol

Self Cite

116

106

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With the growing numbers of nanomaterials (NMs), there is a great demand for rapid and reliable ways of testing NM safety—preferably using in vitro approaches, to avoid the ethical dilemmas associated with animal research. Data are needed for developing intelligent testing strategies for risk assessment of NMs, based on grouping and read‐across approaches. The adoption of high throughput screening (HTS) and high content analysis (HCA) for NM toxicity testing allows the testing of numerous materials at different concentrations and on different types of cells, reduces the effect of inter‐experimental variation, and makes substantial savings in time and cost. HTS/HCA approaches facilitate the classification of key biological indicators of NM‐cell interactions. Validation of in vitro HTS tests is required, taking account of relevance to in vivo results. HTS/HCA approaches are needed to assess dose‐ and time‐dependent toxicity, allowing prediction of in vivo adverse effects. Several HTS/HCA methods are being validated and applied for NM testing in the FP7 project NANoREG, including Label‐free cellular screening of NM uptake, HCA, High throughput flow cytometry, Impedance‐based monitoring, Multiplex analysis of secreted products, and genotoxicity methods—namely High throughput comet assay, High throughput in vitro micronucleus assay, and γH2AX assay. There are several technical challenges with HTS/HCA for NM testing, as toxicity screening needs to be coupled with characterization of NMs in exposure medium prior to the test; possible interference of NMs with HTS/HCA techniques is another concern. Advantages and challenges of HTS/HCA approaches in NM safety are discussed. WIREs Nanomed Nanobiotechnol 2017, 9:e1413. doi: 10.1002/wnan.1413For further resources related to this article, please visit the WIREs website.

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The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles

Cited by 23 publications

References 59 publications

Titanium Dioxide Nanoparticles Increase Superoxide Anion Production by Acting on NADPH Oxidase

Titanium Dioxide Nanoparticles Increase Superoxide Anion Production by Acting on NADPH Oxidase

Simplified TiO2 force fields for studies of its interaction with biomolecules

High throughput toxicity screening and intracellular detection of nanomaterials

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