The effect of bone marrow mesenchymal stem cell and nano‑hydroxyapatite/collagen I/poly‑L‑lactic acid scaffold implantation on the treatment of avascular necrosis of the femoral head in rabbits

Wang, Le; Xu, Leixin; Peng, Changliang; Teng, Guangju; Wang, Yu; Xie, Xiaoxiao; Wu, Dongjin

doi:10.3892/etm.2019.7800

Cited by 8 publications

(10 citation statements)

References 24 publications

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“…In general, the obtained results on bone tissue regeneration are in a good agreement with the results published by other researchers for the application of scaffolds based on aliphatic polyesters. For instance, 23% and 34% of bone tissue volume 2 months after impanation of the PLA and PLA/BMP‐2 scaffolds was detected by Bhattarai et al 60 Wang et al reported 20% and 30% of bone coverage after 4 weeks of implantation of the PLA‐based scaffolds containing nano‐hydroxyapatite/collagen I with and without pre‐adhered MSCs, respectively 61 . The latter are comparable with our results for composite scaffolds which demonstrated 28 and 33% of bone volume a month after implantation.…”

Section: Discussionmentioning

confidence: 98%

3D‐Printed composite scaffolds based on poly(ε‐caprolactone) filled with poly(glutamic acid)‐modified cellulose nanocrystals for improved bone tissue regeneration

et al. 2022

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The manufacturing of modern scaffolds with customized geometry and personalization has become possible due to the three‐dimensional (3D) printing technique. A novel type of 3D‐printed scaffolds for bone tissue regeneration based on poly(ε‐caprolactone) (PCL) filled with nanocrystalline cellulose modified by poly(glutamic acid) (PGlu‐NCC) has been proposed in this study. The 3D printing set‐ups were optimized in order to obtain homogeneous porous scaffolds. Both polymer composites and manufactured 3D scaffolds have demonstrated mechanical properties suitable for a human trabecular bone. Compression moduli were in the range of 334–396 MPa for non‐porous PCL and PCL‐based composites, and 101–122 MPa for porous scaffolds made of the same materials. In vitro mineralization study with the use of human mesenchymal stem cells (hMSCs) revealed the larger Ca deposits on the surface of PCL/PGlu‐NCC composite scaffolds. Implantation of the developed 3D scaffolds into femur of the rabbits was carried out to observe close and delayed effects. The histological analysis showed the lowest content of immune cells and thin fibrous capsule, revealing low toxicity of the PCL/PGlu‐NCC scaffolds seeded with rabbit MSCs (rMSCs) to the surrounding tissues. The most pronounced result on the generation of new bone tissue after implantation of PCL/PGlu‐NCC + rMSCs scaffolds was detected by both microcomputed tomography and histological analysis. Around 33% and 55% of bone coverage were detected for composite 3D scaffolds with adhered rMSCs after 1 and 3 months of implantation, respectively. This achievement can be a result of synergistic effect of PGlu, which attracts calcium ions, and stem cells with osteogenic potential.

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Section: Discussionmentioning

confidence: 98%

3D‐Printed composite scaffolds based on poly(ε‐caprolactone) filled with poly(glutamic acid)‐modified cellulose nanocrystals for improved bone tissue regeneration

et al. 2022

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“…When studied in vivo, angiogenesis was observed near the necrotic bone of the femoral head, and new osteoid tissue was formed. Those results provided support of the use of a polymeric scaffold as an effective treatment for AVN of femoral head [130]. Another study carried out by Luo et al showed BMSCs seeded onto lithium calcium polyphosphate (LiCPP) scaffold containing many other growth factors to be effective in treating AVN because of its osteogenic and angiogenic activity [79].…”

Section: Ceramic/polymeric Scaffoldsmentioning

confidence: 79%

Tissue Engineering Strategies for Treating Avascular Necrosis of the Femoral Head

et al. 2021

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Avascular necrosis (AVN) of the femoral head commonly leads to symptomatic osteoarthritis of the hip. In older patients, hip replacement is a viable option that restores the hip biomechanics and improves pain but in pediatric, adolescent, and young adult patients hip replacements impose significant activity limitations and the need for multiple revision surgeries with increasing risk of complication. Early detection of AVN requires a high level of suspicion as diagnostic techniques such as X-rays are not sensitive in the early stages of the disease. There are multiple etiologies that can lead to this disease. In the pediatric and adolescent population, trauma is a commonly recognized cause of AVN. The understanding of the pathophysiology of the disease is limited, adding to the challenge of devising a clinically effective treatment strategy. Surgical techniques to prevent progression of the disease and avoid total hip replacement include core decompression, vascular grafts, and use of bone-marrow derived stem cells with or without adjuncts, such as bisphosphonates and bone morphogenetic protein (BMP), all of which are partially effective only in the very early stages of the disease. Further, these strategies often only improve pain and range of motion in the short-term in some patients and do not predictably prevent progression of the disease. Tissue engineering strategies with the combined use of biomaterials, stem cells and growth factors offer a potential strategy to avoid metallic implants and surgery. Structural, bioactive biomaterial platforms could help in stabilizing the femoral head while inducing osteogenic differentiation to regenerate bone and provide angiogenic cues to concomitantly recover vasculature in the femoral head. Moreover, injectable systems that can be delivered using a minimal invasive procedure and provide mechanical support the collapsing femoral head could potentially alleviate the need for surgical interventions in the future. The present review describes the limitations of existing surgical methods and the recent advances in tissue engineering that are leading in the direction of a clinically effective, translational solution for AVN in future.

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“…PLA is also one of the most widely used synthetic polymers approved by the FDA for biomedical purposes (Abdalhay et al, 2013;Zhao et al, 2019b;Zhao et al, 2021). Wang et al (2019a) combined PLA, nano-hydroxyapatite, and collagen PLA to establish a nano-hydroxyapatite/collagen I/poly-L-lactic acid composite scaffold (nHAC/PLA). The BMMSC were cultured on the composite scaffold and implanted into the necrotic femoral head after CD in the ANFH rabbit model (Figure 8A).…”

Section: Poly(lactic Acid)mentioning

confidence: 99%

Section: Functionalized Polymer Materials In Osteonecrosis Therapymentioning

confidence: 99%

Polymer Scaffolds-Enhanced Bone Regeneration in Osteonecrosis Therapy

Dong

Zhu

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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Osteonecrosis without effective early treatment eventually leads to the collapse of the articular surface and causes arthritis. For the early stages of osteonecrosis, core decompression combined with bone grafting, is a procedure worthy of attention and clinical trial. And the study of bone graft substitutes has become a hot topic in the area of osteonecrosis research. In recent years, polymers have received more attention than other materials due to their excellent performance. However, because of the harsh microenvironment in osteonecrosis, pure polymers may not meet the stringent requirements of osteonecrosis research. The combined application of polymers and various other substances makes up for the shortcomings of polymers, and to meet a broad range of requirements for application in osteonecrosis therapy. This review focuses on various applying polymers in osteonecrosis therapy, then discusses the development of biofunctionalized composite polymers based on the polymers combined with different bioactive substances. At the end, we discuss their prospects for translation to clinical practice.

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The effect of bone marrow mesenchymal stem cell and nano‑hydroxyapatite/collagen I/poly‑L‑lactic acid scaffold implantation on the treatment of avascular necrosis of the femoral head in rabbits

Cited by 8 publications

References 24 publications

3D‐Printed composite scaffolds based on poly(ε‐caprolactone) filled with poly(glutamic acid)‐modified cellulose nanocrystals for improved bone tissue regeneration

3D‐Printed composite scaffolds based on poly(ε‐caprolactone) filled with poly(glutamic acid)‐modified cellulose nanocrystals for improved bone tissue regeneration

Tissue Engineering Strategies for Treating Avascular Necrosis of the Femoral Head

Polymer Scaffolds-Enhanced Bone Regeneration in Osteonecrosis Therapy

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