The Effect of Bordetella Pertussis Lymphocytosis-Promoting Factor (Lpf) on Antibody Response in Mice: Its Enhancing and Suppressive Effects

Abstract

SUMMARY: The effect of lymphocytosis-promoting factor (LPF) on antibody response in mice was estimated under different sets of experimental conditions. Four-and 6-week-old mice were intravenously inoculated with LPF. Three days later these mice were inoculated either intraperitoneally or intravenously with sheep red blood cell (SRBC) or human serum albumin (HSA) as an antigen.The adjuvant effect of LPF was demonstrated on antibody response in 6-week old mice to intraperitoneally inoculated SRBC but not to intravenously-inoculated one. When 4-week-old mice were immunized, hemagglutinin production in response to intraperitoneally inoculated SRBC was not enhanced by LPF. In addition, a rather suppressive effect of LPF at a comparatively high dose was demonstrated on hemagglutinin production in response to intravenously inoculated SRBC.Anti-HSA production was enhanced by inoculation of LPF in any combination of the mouse age and the route of antigen administration.These findings indicate that the adjuvant effect of LPF on antibody response in mice depends upon experimental conditions: the age of mice, the quality of antigen and the route of antigen administration used for immunization.Several reports have shown that LPF of Bordetella pertussis acts as an immunomodulator in humoral and cellular immunity. In these reports, however, conflicting results were given in respect to the effect of LPF on antibody response in mice. Asakawa (1969) demonstrated that antibody response of mice to SRBC and tetanus toxoid was suppressed by inoculation of LPF prior to the antigen, whereas some other workers observed an increased antibody response when LPF was mixed with the antigen or given before the administration of antigen. It is possible that the above contradictions may have been due to some different experimental conditions used by them. To test this possibility, we compared the erect of LPF on antibody response in mice under several different sets of well-defined experimental conditions. The LPF preparation used had been partially purified by the method de-

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

“…This is probably due to the presence of transplacentally acquired antibody to PT. PT is a strong adjuvant in several immunologic systems in several animals and humans (14,15,190,235,246,452,453,456,553,561,573,575,578,600,610,649,699,745,746,801,804). This adjuvancy in the experimental-animal model is associated with enhancement of serum antibody responses to other antigens, increased cellular immune responses to various protein antigens, contribution to hyperacute experimental autoallergic encephalomyelitis, and increased anaphylactic sensitivity.…”

In vitro inhibition of murine macrophage migration by Bordetella pertussis lymphocytosis-promoting factor

“…It has been proposed that LPF is responsible for the systemic manifestations of B. pertussis infection (37,49), and parallels have been observed between the effects of purified LPF in experimental animals and those signs observed in infected humans or animals. For example, administration of LPF sensitizes mice to the lethal effects of histamine (30,31,52), induces leukocytosis and lymphocytosis (30,31), enhances the release of insulin (52), inhibits epinephrine-induced hyperglycemia (30,52), and is an immunological adjuvant (2,31). Mice with respiratory infections show leukocytosis, histamine sensitization, and enhanced insulin release (15,38,40).…”