The Effect of Bovine Herpesvirus Type 1 Glycoproteins gI and gIII on Herpesvirus Infections

Chase, Christopher; Carter-Allen, Kelly; Letchworth, Geoffrey J.

doi:10.1099/0022-1317-70-6-1561

Cited by 13 publications

(9 citation statements)

References 25 publications

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“…It is particularly noteworthy that the fusogenic activity associated with gI expression, observed previously in transfected LMTK-cells (Fitzpatrick et al, 1988), was reproducible in transfected MDBK cells. This result suggests that gI-associated cell fusion is not species-specific, as recently suggested (Chase et al, 1989) and that it is not dependent on the abnormal intracellular distribution of an atypical recombinant molecule. The lack of cell fusion in gI-expressing, transfected bovine fibroblasts noted by Chase et al (1989) may be due to a number of factors, including cell-specific differences between MDBK cells and bovine fibroblasts, use of an inducible rather than a constitutive expression system, different cell culture conditions during selection and cloning procedures, and/or quantitative differences in gI expression levels.…”

supporting

confidence: 59%

“…Conclusions regarding the biological relevance of this phenomenon were circumspect due to the unusual features of expression of gI in transfected LMTK-cells, which were pronounced perinuclear accumulation of recombinant gI, altered N-linked glycosylation of the amino-terminal cleavage fragment of recombinant gI, and complete proteolytic cleavage of recombinant gI (Fitzpatrick et al, 1988). Recently, others have failed to detect fusion of transfected bovine fibroblasts expressing gI and have suggested that gI-associated cell fusion may be species-specific (Chase et al, 1989).…”

mentioning

confidence: 99%

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Expression of Bovine Herpesvirus Type 1 Glycoprotein gI in Transfected Bovine Cells Induces Spontaneous Cell Fusion

FitzPatrick

Zamb²,

Babiuk

1990

Journal of General Virology

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Bovine MDBK cells were transfected with Rous sarcoma virus-based vectors for constitutive expression of the bovine herpesvirus type 1 (BHV-1) glycoprotein, gI. Cell lines stably expressing recombinant gI were cloned and characterized. Recombinant gI was localized intracellularly, predominantly in a perinuclear region, and on the cell surface. Cells expressing gI exhibited spontaneous polykaryon formation, thus confirming the fusogenic activity described previously in gI-expressing transfected murine LMTK-cells. The recombinant form of gI synthesized in transfected MDBK cells was similar in Mr to the form expressed in BHV-l-infected MDBK cells, unlike the recombinant form ofgI expressed by LMTK-cells which is deficient in N-linked glycosylation. It was concluded that cell fusion associated with the expression of BHV-1 gI in transfected mammalian cells is a reproducible phenomenon in a number of cell types and is not due to species-specific factors or expression of abnormally glycosylated gI. Cell fusion is a useful in vitro marker for gI function and may contribute to the spread of BHV-1 infections in vivo.

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supporting

confidence: 59%

mentioning

confidence: 99%

Expression of Bovine Herpesvirus Type 1 Glycoprotein gI in Transfected Bovine Cells Induces Spontaneous Cell Fusion

FitzPatrick

Zamb²,

Babiuk

1990

Journal of General Virology

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“…It has three major envelope glycoproteins; gB, gC, and gD, which are involved in attachment (gB, gC and gD) and penetration (gB and gD) of BHV-1 into host cells (37, 41). Although all these glycoproteins are effective immunogens and can induce significant protection from virulent field challenge (4, 12, 20, 56, 61), gD is considered a major target for neutralizing antibodies and cytotoxic T lymphocytes (4, 15, 44, 56, 57). Several studies have been conducted to use gD in DNA or subunit vaccines to induce protective immune responses against BHV-1 on mucosal surfaces.…”

Section: Introductionmentioning

confidence: 99%

Immunization of cattle with recombinant Newcastle disease virus expressing bovine herpesvirus-1 (BHV-1) glycoprotein D induces mucosal and serum antibody responses and provides partial protection against BHV-1

Khattar

Collins

Samal

2010

Vaccine

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Bovine herpesvirus-1 (BHV-1) is a major cause of respiratory tract diseases in cattle. Vaccination of cattle against BHV-1 is a high priority. A major concern of currently modified live BHV-1 vaccines is their ability to cause latent infection and subsequent reactivation resulting in many outbreaks. Thus, there is a need for alternative strategies. We generated two recombinant Newcastle disease viruses (NDVs) expressing the glycoprotein D (gD) of BHV-1 from an added gene. One recombinant, rLaSota/gDFL, expressed gD without any modification. The other recombinant, rLaSota/gDF, expressed a chimeric gD in which the ectodomain of gD was fused with the transmembrane domain and cytoplasmic tail of the NDV fusion F glycoprotein. Remarkably, the native gD expressed by rLaSota/gDFL virus was incorporated into the NDV virion 2.5-fold more efficiently than the native NDV proteins, whereas the chimeric gD was not detectably incorporated even though it was abundantly expressed on the infected cell surface. The expression of gD did not increase the virulence of the rNDV vectors in chickens. A single intranasal and intratracheal inoculation of calves with either recombinant NDV elicited mucosal and systemic antibodies specific to BHV-1, with the responses to rLaSota/gDFL being higher than those to rLaSota/gDF. Following challenge with BHV-1, calves immunized with the recombinant NDVs had lower titers and earlier clearance of challenge virus compared to the empty vector control, and reduced disease was observed with rLaSota/gDFL. Following challenge, the titers of serum antibodies specific to BHV-1 were higher in the animals immunized with the rNDV vaccines compared to the rNDV parent virus, indicating that the vaccines primed for secondary responses. Our data suggest that NDV can be used as a vaccine vector in bovines and that BHV-1 gD may be useful in mucosal vaccine against BHV-1 infection, but might require augmentation by a second dose or the inclusion of additional BHV-1 antigens.

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“…However, the specific glycoprotein-based ELISA has not been commercially available for rapid detection of seroconversion by vectored vaccines against ILTV. Therefore, in the present study, we developed rapid diagnostic ELISAs by using ILTV gB, gC, and gD (B-, C-, and D-ELISAs, respectively) as antigens, since these glycoproteins can induce humoral and cellmediated immune responses against ILTV and other herpesviruses (7,(11)(12)(13)(14). Each glycoprotein was eukaryotically expressed in insect cells.…”

mentioning

confidence: 99%

Glycoprotein-Based Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Infectious Laryngotracheitis

et al. 2015

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Infectious laryngotracheitis virus (ILTV), an alphaherpesvirus, causes an acute respiratory disease in chickens known as infectious laryngotracheitis (ILT) that leads to significant economic losses to the poultry industry worldwide (1, 2). Currently, live attenuated vaccines are used to control ILTV infections. However, these vaccines are not satisfactory since the vaccine virus can revert to virulence after bird-to-bird passage (3) and can induce latent infections (4). Due to these limitations, live virus-vectored vaccines expressing the surface glycoproteins of ILTV have been developed as a safer alternative to attenuated live vaccines. A recombinant herpesvirus of turkey (HVT-LT) expressing ILTV glycoproteins D (gD) and I (gI) and Fowl pox virus (FPV-LT) expressing ILTV glycoprotein B (gB) and UL-34 genes are commercially used in the United States (5, 6). Recently, we have evaluated the role of ILTV gB, gC, and gD in immunogenicity and protection against a virulent ILTV challenge using recombinant Newcastle disease virus (rNDV) as a vaccine vector. Our results indicated that rNDV expressing ILTV gD provided complete protection against a virulent ILTV challenge in chickens (7). A vectored vaccine against ILTV infection will be safe without reversion of vaccine virus to be virulent or establishment of latency and also allow differentiation of vaccinated birds from the infected birds.The detection of the humoral immune response is critical for the rapid identification of ILTV-infected birds (5). The enzymelinked immunosorbent assay (ELISA) has been used for the detection of the humoral immune response. Despite its simplicity and rapidity, the commercially available ELISA using whole virus as an antigen is inefficient for detecting seroconversion with virus-vectored vaccines (6). Recently, individual ILTV surface glycoproteins have been used for ELISAs to detect ILTV antibodies in sera from vaccinated birds with attenuated and vectored vaccines against ILT (8-10). However, the specific glycoprotein-based ELISA has not been commercially available for rapid detection of seroconversion by vectored vaccines against ILTV. Therefore, in the present study, we developed rapid diagnostic ELISAs by using ILTV gB, gC, and gD (B-, C-, and D-ELISAs, respectively) as antigens, since these glycoproteins can induce humoral and cellmediated immune responses against ILTV and other herpesviruses (7,(11)(12)(13)(14). Each glycoprotein was eukaryotically expressed in insect cells. The diagnostic potential of these ELISAs was assayed with sera collected from chickens vaccinated with various virus-vectored vaccines. Furthermore, the efficacy of our ELISAs was validated by testing field serum samples and compared to that of a commercially available ELISA as a reference (fowl laryngotracheitis virus antibody test kit; Zoetis, San Diego, CA) (15-17).The ILTV gB, gC, and gD genes were cloned into the pCR 4 TOPO vector (Invitrogen) as described previously (7), and these genes were amplified from the TOPO vectors with concurrent introduction...

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The Effect of Bovine Herpesvirus Type 1 Glycoproteins gI and gIII on Herpesvirus Infections

Cited by 13 publications

References 25 publications

Expression of Bovine Herpesvirus Type 1 Glycoprotein gI in Transfected Bovine Cells Induces Spontaneous Cell Fusion

Expression of Bovine Herpesvirus Type 1 Glycoprotein gI in Transfected Bovine Cells Induces Spontaneous Cell Fusion

Immunization of cattle with recombinant Newcastle disease virus expressing bovine herpesvirus-1 (BHV-1) glycoprotein D induces mucosal and serum antibody responses and provides partial protection against BHV-1

Glycoprotein-Based Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Infectious Laryngotracheitis

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