The Effect of Bovine IFN-&amp;alpha; on the Immune Response in Guinea Pigs Vaccinated with DNA Vaccine of Foot-and-Mouth Disease Virus

Guo, Huichen; Liu, Zaixin; Sun, Shiqi; Qing-wen, Leng; Li, Dong; Liu, Xiangtao; Xie, Qing-Ge

doi:10.1093/abbs/36.10.701

Cited by 12 publications

(8 citation statements)

References 23 publications

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“…The antibody titers in immunized guinea pigs were determined by indirect-ELISA as described elsewhere [36,37]. Briefly, 96-well plates were coated with inactivated FMDV Asia1/Jiangsu/China/2005 (100 mL/well) in 0.05 M bicarbonate buffer (pH 9.6) at 4°C overnight.…”

Section: Methodsmentioning

confidence: 99%

Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle

Guo

Sun

Jin

et al. 2013

Vet Res

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Foot-and-mouth disease virus (FMDV) causes a highly contagious infection in cloven-hoofed animals. The format of FMD virus-like particles (VLP) as a non-replicating particulate vaccine candidate is a promising alternative to conventional inactivated FMDV vaccines. In this study, we explored a prokaryotic system to express and assemble the FMD VLP and validated the potential of VLP as an FMDV vaccine candidate. VLP composed entirely of FMDV (Asia1/Jiangsu/China/2005) capsid proteins (VP0, VP1 and VP3) were simultaneously produced as SUMO fusion proteins by an improved SUMO fusion protein system in E. coli. Proteolytic removal of the SUMO moiety from the fusion proteins resulted in the assembly of VLP with size and shape resembling the authentic FMDV. Immunization of guinea pigs, swine and cattle with FMD VLP by intramuscular inoculation stimulated the FMDV-specific antibody response, neutralizing antibody response, T-cell proliferation response and secretion of cytokine IFN-γ. In addition, immunization with one dose of the VLP resulted in complete protection of these animals from homologous FMDV challenge. The 50% protection dose (PD50) of FMD VLP in cattle is up to 6.34. These results suggest that FMD VLP expressed in E. coli are an effective vaccine in guinea pigs, swine and cattle and support further development of these VLP as a vaccine candidate for protection against FMDV.

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Section: Methodsmentioning

confidence: 99%

Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle

Guo

Sun

Jin

et al. 2013

Vet Res

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“…Guo used bovine IFN-a and DNA Vaccine of Foot-andMouth Disease Virus to vaccinate Guinea pigs. The result indicated that IFN-a could enhance the effect of T-lymphocytes proliferation (Guo et al, 2004). In our study, we used pcDNA-SDIFN-a to inoculate 28-day-old ducks at different doses by gene gun.…”

Section: Effect Of T-lymphocytes Proliferationmentioning

confidence: 90%

Effect of cell mediated immunity regulation of duck enhanced by duck IFN-α eukaryon expression plasmid and inoculated with DPV attenuated vaccine by gene-gun

Zeng

Cheng

Wang

et al. 2008

Front. Agric. China

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In order to study the effect of cell mediated immunity regulation of duck IFN-a eukaryon expression plasmid (pcDNA-SDIFN-a) on duck plague virus (DPV) attenuated vaccine in ducks, pcDNA-SDIFN-a was administered to 28-day-old ducks at doses of 1, 3 and 6 mg per duck, respectively, by gene-gun. PBS and empty vector pcDNA were used as control. Fifteen days later, all ducks were injected with DPV attenuated vaccine and blood samples were collected at 3, 7, 14, 21, 28, 35, 49, 63 and 84 days after injection. T-lymphocyte proliferation tests (MTT) were used to detect the T-lymphocyte proliferation in the peripheral blood (PBL) of ducks. Blood samples collected at 7, 14, 21, 28, 35 and 49 days after injection were detected by fluorescence-activated cell sorter (FACS) for recording the number of CD 3 + T-lymphocytes of ducks. Results were as follows: (1) Reaction of T-lymphocytes in PBL to ConA (OD value) of ducks treated with pcDNA-SDIFN-a was higher than that of PBS and pcDNA control groups in 3-84 days. There were highly significant differences between the 1 mg per duck group and the two control groups in 3-84 days (P ( 0.01), between the 3 mg per duck group and the two control groups in 3-84 days (P ( 0.01, P ( 0.05), and between the 6 mg per duck group and the two control groups in 7-49 days (P ( 0.01, P ( 0.05). The significant difference was also present between the groups of 1, 3 and 6 mg per duck in 3-35 days (P ( 0.05). However, there was no significant difference between the 3 and 6 mg per duck groups (P > 0.05). The pcDNA control group was higher than PBS control group, but no difference was detected (P > 0.05).(2) Change of the number of CD 3 + T-lymphocytes in ducks administered with different doses of pcDNA-SDIFN-a was higher than that of PBS and pcDNA control groups in 7-49 days. The change in the 1 mg per duck group was significantly higher than that in PBS and pcDNA control groups in 14-49 days (P ( 0.01). There were significant differences between the 3 mg per duck group and the two control groups in 21-49 days (P ( 0.01, P ( 0.05) and between the 6 mg per duck group and the two control groups in 7-49 days (P ( 0.01, P ( 0.05). However, no significant differences among the groups of 1, 3, and 6 mg per duck groups (P > 0.05) and between the two control groups (P > 0.05) were found. The results indicated that pcDNA-SDIFN-a administered 15 days before injection of DPV-attenuated vaccine could significantly enhance cellular immunity induced by DPV-attenuated vaccine. pcDNA-SDIFN-a is an excellent DPV-attenuated vaccine molecular adjuvant and the best result can be obtained with the dose of 1 mg per duck of pcDNA-SDIFN-a inoculated by gene-gun.

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“…After cloning the HN region in the eukaryotic expression vector pcDNA3.1, which is commonly used for high-level stable expression in mammalian cells [27-30], its expression was analyzed in Vero-transfected cells. We found that the pcDNAHN176-construct over expressed the corresponding RNA and polypeptide.…”

Section: Discussionmentioning

confidence: 99%

A 176 amino acid polypeptide derived from the mumps virus HN ectodomain shows immunological and biological properties similar to the HN protein

Herrera¹,

Bárcenas²,

Hernández³

et al. 2010

Virol J

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BackgroundThe hemagglutinin-neuraminidase (HN) protein is the major antigenic determinant of the Mumps virus (MuV) and plays an important role in the viral infectious cycle through its hemagglutination/hemadsorption (HA/HD) and neuraminidase (NA) activities. Objective: analyze the biological and immunological properties of a polypeptide derived from a highly conserved region of the HN ectodomain. Methods: a highly conserved region of the HN gene among several MuV genotypes was chosen to be cloned in a eukaryotic expression vector. The pcDNAHN176-construct was transfected into Vero cells and RNA expression was detected by RT-PCR, while the corresponding polypeptide was detected by immunofluorescence and immunochemistry techniques. The HD and NA activities were also measured. The immunogenic properties of the construct were evaluated using two systems: rabbit immunization to obtain sera for detection of the HN protein and neutralization of MuV infection, and hamster immunization to evaluate protection against MuV infection.ResultsA 567 nucleotide region from the HN gene was amplified and cloned into the plasmid pcDNA3.1. Vero cells transfected with the construct expressed a polypeptide that was recognized by a MuV-hyperimmune serum. The construct-transfected cells showed HD and NA activities. Sera from immunized rabbits in vitro neutralized two different MuV genotypes and also detected both the HN protein and the HN176 polypeptide by western blot. Hamsters immunized with the pcDNAHN176-construct and challenged with MuV showed a mild viral infection in comparison to non-immunized animals, and Th1 and Th2 cytokines were detected in them.ConclusionsThe pcDNAHN176-construct was capable of expressing a polypeptide in Vero cells that was identified by a hyperimmune serum anti Mumps virus, and these cells showed the HD and NA activities of the complete MuV HN protein. The construct also elicited a specific immune response against MuV infection in hamsters.

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The Effect of Bovine IFN-α on the Immune Response in Guinea Pigs Vaccinated with DNA Vaccine of Foot-and-Mouth Disease Virus

Cited by 12 publications

References 23 publications

Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle

Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle

Effect of cell mediated immunity regulation of duck enhanced by duck IFN-α eukaryon expression plasmid and inoculated with DPV attenuated vaccine by gene-gun

A 176 amino acid polypeptide derived from the mumps virus HN ectodomain shows immunological and biological properties similar to the HN protein

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