Microbial remediation
of fracturing fluid damage is for the first
time to be presented in this paper. One guar gum degrading bacterial
strain, Bacillus sonorensis, numbered by XSJ, was
isolated from oilfield produced water and identified through 16S rDNA.
The characterizations show that strain XSJ is mesophilic, alkalophilic,
facultative, and halophilic, which is suitable for the reservoir environment.
In addition, the guar gum degrading performance of this strain was
investigated through apparent viscosity of the guar gum solution,
the average molecular weight of guar gum, and gas chromatography.
Besides, the degrading performance of insoluble residue of guar gum
was analyzed through the measurement of particle size distribution
using a laser particle size analyzer and total weight of insoluble
residue using a weighing method. Finally, a kind of sand-pack column
was designed to determine the recovery of permeability. The results
indicate the following: (1) Bacillus sonorensis can
efficiently degrade guar-based fracturing fluids to less than 5.0
mPa·s of apparent viscosity and 50 000 average molecular
weight. (2) The bacterial strain can decrease the average particle
size of insoluble residue from 105.56 to 72.40 μm and total
weight of insoluble residue with the microbial degradation rate of
25.30%. (3) This strain can improve effectively the permeability of
sand-pack column (max 90.32%) in laboratory simulation experiments.
In conclusion, it is believed that the microbial remediation seems
to be a new approach for remediation of guar-based fracturing fluid
damage and has great potential in oilfield application.