The Effect of Butyrate on the Healing of Colonic Anastomoses in Rats

Mathew, Amrith; Wann, Veracious Cornerstone; Abraham, Devaprabu; Jacob, Petra; Selvan, B S; Ramakrishna, B. S.; Nair, Amit

doi:10.3109/08941930903469367

Cited by 24 publications

(20 citation statements)

References 19 publications

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“…Since then, butyrate enemas have popularly been used as medicaments stemming from their potential to impart beneficial attributes to the colon. This potential involves an increase in mechanical strength of injured colonic mucosa to hasten the healing process (Bloemen et al, 2010;Mathew et al, 2010), suppression of IL-8 production by intestinal epithelial cells to protect against the inflammatory process (Malago et al, 2005), and clinical remission of UC by protecting against inflammatory and oxidative stress parameters of the disease (Hamer et al, 2010b). Much as butyrate tends to impart a protective effect, several authors have indicated failures or limited success of butyrate to relieve IBD patients (Harig et al, 1989;Sanderson, 1997;Hamer et al, 2010b).…”

Section: Introductionmentioning

confidence: 99%

Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats

Malago

Sangu²

2015

J. Zhejiang Univ. Sci. B

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Intrarectal infusion of butyrate improves colorectal disorders including ulcerative colitis (UC). However, it is not established whether systemically administered butyrate benefits such patients. The current study aimed at exploring and comparing the potential of intraperitoneally, intrarectally, and orally administered butyrate against acetic acid (AA)-induced UC in rats. Intrarectal administration of 2 ml of 50% AA was done after or without prior treatment of rats for 7 consecutive days with 100 mg/kg sodium butyrate (SB) intraperitoneally, intrarectally, or orally. Rats were sacrificed after 48 h of AA-treatment. Subsequently, colon sections were processed routinely for histopathological examination. We clinically observed diarrhea, loose stools, and hemoccult-positive stools, and histologically, epithelial loss and ulceration, crypt damage, goblet cell depletion, hemorrhage, and mucosal infiltration of inflammatory cells. The changes were significantly reduced by intraperitoneal, intrarectal, or oral butyrate, with intraperitoneal butyrate exhibiting the highest potency. It is concluded that intraperitoneal administration of butyrate abrogates the lesions of AA-induced UC and its potency surpasses that of intrarectal or oral butyrate.

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Section: Introductionmentioning

confidence: 99%

Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats

Malago

Sangu²

2015

J. Zhejiang Univ. Sci. B

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“…24 This was corroborated by another group which similarly found strengthened bursting wall tension in those subjects receiving butyrate enemas postoperatively after right and left colon resection. 25 To look at this pathophysiology further, Levison demonstrated that SCFAs inhibited in vitro growth of P. aeruginosa 26 exactly the pathogen that was later identified as being able to transform into tissue destroying phenotype with high collagenase activity, as previously discussed. 18 Taken together, it tends to reason that the gut microbiome may additionally influence anastomotic healing by production of SCFAs, namely butyrate, which have beneficial effects on anastomotic integrity, and possibly limiting the growth of deleterious bacterial pathogens.…”

Section: Butyratementioning

confidence: 93%

Gut Microbiota and Colorectal Surgery: Impact on Postoperative Complications

Russ

Casillas

2016

Clinics in Colon and Rectal Surgery

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“…Previous animal studies have shown that rectal administration of butyrate—one of the predominant short chain fatty acids (SCFAs)—results in a higher bursting pressure in colonic anastomoses, thereby claiming an enhanced strength which may prevent anastomotic leakage [ 6 – 8 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of three different application routes of butyrate to improve colonic anastomotic strength in rats

Bosmans

Jongen

Boonen

et al. 2016

Int J Colorectal Dis

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IntroductionDespite extensive research, anastomotic leakage (AL) remains one of the most dreaded complications after colorectal surgery. Since butyrate enemas are known to enhance anastomotic healing, several administration routes have been explored in this study.MethodsThree intraluminal approaches involving butyrate were investigated: (1) butyrin-elucidating patch, (2) a single injection of hyaluronan-butyrate (HA-But) prior to construction of the proximal anastomosis and (3) rectal hyaluronan-butyrate (HA-But) enemas designed for distal anastomoses. The main outcome was AL and secondary outcomes were bursting pressure, histological analysis of the anastomosis, zymography to detect MMP activity and qPCR for gene expression of MMP2, MMP9, MUC2 and TFF3.ResultsNeither the patches nor the injections led to a reduction of AL in experiments 1 and 2. In experiment 3, a significant reduction of AL was accomplished with the (HA-But) enema compared to the control group together with a higher bursting pressure. Histological analysis detected only an increased inflammation in experiment 2 in the hyaluronan injection group compared to the control group. No other differences were found regarding wound healing. Zymography identified a decreased proenzyme of MMP9 when HA-But was administered as a rectal enema. qPCR did not show any significant differences between groups in any experiment.ConclusionButyrate enemas are effective in the enhancement of colonic anastomosis. Enhanced butyrate-based approaches designed to reduce AL in animal models for both proximal and distal anastomoses were not more effective than were butyrate enemas alone. Further research should focus on how exogenous butyrate can improve anastomotic healing after gastrointestinal surgery.Electronic supplementary materialThe online version of this article (doi:10.1007/s00384-016-2718-z) contains supplementary material, which is available to authorized users.

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The Effect of Butyrate on the Healing of Colonic Anastomoses in Rats

Abstract: This experiment suggests that butyrate has a significant role in increasing the mechanical strength of colonic anastomoses in rats.

Cited by 24 publications

References 19 publications

Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats

Intraperitoneal administration of butyrate prevents the severity of acetic acid colitis in rats

Gut Microbiota and Colorectal Surgery: Impact on Postoperative Complications

Comparison of three different application routes of butyrate to improve colonic anastomotic strength in rats

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