The effect of C/N ratio on ammonia oxidising bacteria community structure in a laboratory nitrification-denitrification reactor

Ballinger, S. J.; Head, Ian M; Curtis, Thomas P.; Godley, A.

doi:10.2166/wst.2002.0532

Cited by 38 publications

(22 citation statements)

References 7 publications

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“…Many physical and geochemical factors influence nitrification, including oxygen concentrations (e.g. Christensen et al ., 1990), sulfide concentrations (Joye and Hollibaugh, 1995), Fe(III) content (Dollhopf et al ., 2005), salinity (Rysgaard et al ., 1999; Caffrey et al ., 2003; Bernhard et al ., 2007), C:N ratios (Ballinger et al ., 2002), availability of ammonium (e.g. Jones and Hood, 1980), light (Horrigan and Springer, 1990) and temperature (Berounsky and Nixon, 1993).…”

Section: Resultsmentioning

confidence: 99%

Relative abundance and diversity of ammonia‐oxidizing archaea and bacteria in the San Francisco Bay estuary

Mosier¹,

Francis²

2008

Environmental Microbiology

351

356

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Ammonia oxidation in marine and estuarine sediments plays a pivotal role in the cycling and removal of nitrogen. Recent reports have shown that the newly discovered ammonia-oxidizing archaea can be both abundant and diverse in aquatic and terrestrial ecosystems. In this study, we examined the abundance and diversity of ammonia-oxidizing archaea (AOA) and betaproteobacteria (beta-AOB) across physicochemical gradients in San Francisco Bay--the largest estuary on the west coast of the USA. In contrast to reports that AOA are far more abundant than beta-AOB in both terrestrial and marine systems, our quantitative PCR estimates indicated that beta-AOB amoA (encoding ammonia monooxygenase subunit A) copy numbers were greater than AOA amoA in most of the estuary. Ammonia-oxidizing archaea were only more pervasive than beta-AOB in the low-salinity region of the estuary. Both AOA and beta-AOB communities exhibited distinct spatial structure within the estuary. AOA amoA sequences from the north part of the estuary formed a large and distinct low-salinity phylogenetic group. The majority of the beta-AOB sequences were closely related to other marine/estuarine Nitrosomonas-like and Nitrosospira-like sequences. Both ammonia-oxidizer community composition and abundance were strongly correlated with salinity. Ammonia-oxidizing enrichment cultures contained AOA and beta-AOB amoA sequences with high similarity to environmental sequences. Overall, this study significantly enhances our understanding of estuarine ammonia-oxidizing microbial communities and highlights the environmental conditions and niches under which different AOA and beta-AOB phylotypes may thrive.

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Section: Resultsmentioning

confidence: 99%

Relative abundance and diversity of ammonia‐oxidizing archaea and bacteria in the San Francisco Bay estuary

Mosier¹,

Francis²

2008

Environmental Microbiology

351

356

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“…It has been reported that Nitrosomonas were dominant AOB in both wastewater treatment plants Juretschko et al 1998;Dionisi et al 2002;Hallin et al 2005) and bench-scale systems (Okabe et al 1999;Bouchez et al 2000;Ballinger et al 2002;Nogueira et al 2002;Tsuneda et al 2003), while Nitrosospira were dominant mainly in bench-scale systems (Schramm et al 1998(Schramm et al , 1999You et al 2003). It has been reported that Nitrosomonas were dominant AOB in both wastewater treatment plants Juretschko et al 1998;Dionisi et al 2002;Hallin et al 2005) and bench-scale systems (Okabe et al 1999;Bouchez et al 2000;Ballinger et al 2002;Nogueira et al 2002;Tsuneda et al 2003), while Nitrosospira were dominant mainly in bench-scale systems (Schramm et al 1998(Schramm et al , 1999You et al 2003).…”

Section: The Shift Of Dominant Aob and Nob Groupsmentioning

confidence: 98%

The variation of nitrifying bacterial population sizes in a sequencing batch reactor (SBR) treating low, mid, high concentrated synthetic wastewater

Irvin

Baker

2007

Journal of Environmental Engineering and Science

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The purpose of this study was to correlate the population size of ammonia-oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) with nitrification performance under various operational conditions (chemical oxygen demand (COD) concentration, dissolved oxygen (DO), and hydraulic retention time (HRT)) and influent allylthiourea (ATU) shock. The AOB (genera Nitrosomonas and Nitrosospira) and NOB (genera Nitrobacter and Nitrospira) communities were analyzed using fluorescent in situ hybridization (FISH). Ammonia-oxidizing bacteria and NOB accounted for 6.2 ± 0.9% and 2.5 ± 0.3% in total biomass, respectively. The population sizes of AOB and NOB varied with different levels of COD, DO, and HRT. Nitrosomonas and Nitrospira were dominant under conditions favorable for nitrification, while Nitrosospira outcompeted Nitrosomonas under adverse conditions (low [NH4 + ], low DO, short HRT, and ATU shock), and Nitrobacter outcompeted Nitrospira at high substrate concentrations (COD and [NH 4 + ]). Under ATU shock that inhibited the oxidation of NH 4 + to NO 2 -, AOB population was substantially reduced with the stepwise increase of ATU dosage, and led to a corresponding decrease of NOB population. There was a discrepancy between nitrifying bacterial populations and their functions. Although AOB outnumbered NOB in all tests and became more dominant at low DO and short HRT, NH 4 + oxidation, instead of NO 2oxidation, was the rate-limiting reaction for nitrification and susceptible to the adverse conditions. The study demonstrated the importance of elucidating the shifts of nitrifying bacterial population to optimize process design and operation at different influent characteristics, aeration intensity, retention time, and potential influent toxic shock.

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“…Relevant operational parameters of these plants are summarized in Table 1. The laboratory reactor is described in detail elsewhere (4,5).…”

Section: Activated Sludge Plantsmentioning

confidence: 99%

Agreement between Theory and Measurement in Quantification of Ammonia-Oxidizing Bacteria

Coşkuner

Ballinger

Davenport

et al. 2005

Appl Environ Microbiol

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Autotrophic ammonia-oxidizing bacteria (AOB) are of vital importance to wastewater treatment plants (WWTP), as well as being an intriguing group of microorganisms in their own right. To date, corroboration of quantitative measurements of AOB by fluorescence in situ hybridization (FISH) has relied on assessment of the ammonia oxidation rate per cell, relative to published values for cultured AOB. Validation of cell counts on the basis of substrate transformation rates is problematic, however, because published cell-specific ammonia oxidation rates vary by over two orders of magnitude. We present a method that uses FISH in conjunction with confocal scanning laser microscopy to quantify AOB in WWTP, where AOB are typically observed as microcolonies. The method is comparatively simple, requiring neither detailed cell counts or image analysis, and yet it can give estimates of either cell numbers or biomass. Microcolony volume and diameter were found to have a log-normal distribution. We were able to show that virtually all (>96%) of the AOB biomass occurred as microcolonies. Counts of microcolony abundance and measurement of their diameter coupled with a calibration of microcolony dimensions against cell numbers or AOB biomass were used to determine AOB cell numbers and biomass in WWTP. Cell-specific ammonia oxidation rates varied between plants by over three orders of magnitude, suggesting that cell-specific ammonia oxidation is an important process variable. Moreover, when measured AOB biomass was compared with process-based estimates of AOB biomass, the two values were in agreement.The quantification of microbial communities and populations is an invaluable aspect of microbial ecology. In principle, the autotrophic ammonia-oxidizing bacteria (AOB) are ideal candidates for the development of quantitative tools. AOB have a coherent phylogeny and defined nutritional requirements and are of profound practical importance in natural and engineered environments.The number of individuals should be the ideal benchmark for quantitative studies. Individual counts can be converted to biomass, biovolume, or proportion of biomass, and results obtained by more indirect methods are typically compared to the number of cells per unit volume (15). Fluorescence in situ hybridization (FISH) represents the "gold standard" for quantification of specific bacterial cells in the environment, against which other methods should be compared. Classical (27) and immunological (20) methods are subject to methodological biases, while nonmicroscopic 16S rRNA-based methods (8, 34) or PCR-based methods (13,14,18,19) deliver a proportion of total cell counts, copy number, or relative signal intensities rather than an absolute number of cells or biomass.A quantitative method may be evaluated with respect to its precision and its accuracy. Wagner et al. (43) originally evaluated the accuracy of FISH counts of AOB by using cell specific oxidation rates, an approach previously used to show that most-probable-number-based methods underestimate AOB numbers...

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The effect of C/N ratio on ammonia oxidising bacteria community structure in a laboratory nitrification-denitrification reactor

Cited by 38 publications

References 7 publications

Relative abundance and diversity of ammonia‐oxidizing archaea and bacteria in the San Francisco Bay estuary

Relative abundance and diversity of ammonia‐oxidizing archaea and bacteria in the San Francisco Bay estuary

The variation of nitrifying bacterial population sizes in a sequencing batch reactor (SBR) treating low, mid, high concentrated synthetic wastewater

Agreement between Theory and Measurement in Quantification of Ammonia-Oxidizing Bacteria

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